PMID- 35429551
OWN - NLM
STAT- MEDLINE
DCOM- 20220512
LR  - 20220512
IS  - 1873-4863 (Electronic)
IS  - 0168-1656 (Linking)
VI  - 350
DP  - 2022 May 20
TI  - A highly effective strain screened from soil and applied in cementing fine sand 
      based on MICP-bonding technology.
PG  - 55-66
LID - S0168-1656(22)00074-8 [pii]
LID - 10.1016/j.jbiotec.2022.03.016 [doi]
AB  - Soil cementation based on microbially induced calcite precipitation (MICP) 
      technology is a new research hotspot in the field of biogeotechnical engineering. 
      However, due to the presence of only calcium bonds among particles, MICP-cemented 
      soil shows obvious brittleness, which is the bottleneck of the application of 
      MICP technology in geotechnical engineering. MICP-bonding technology can improve 
      the shortcoming of high brittleness of the calcium bond by increasing the plastic 
      bond among matrix particles. Based on MICP-bonding technology, this study 
      isolated a high-efficiency strain named XR1(#), which has the ability to produce 
      both urease and extracellular polysaccharide. Gene sequencing identified the 
      strain as Bacillus oceanicus. The superiority of XR1(#) bacteria is its ability 
      to induce mineralization and polysaccharide production at the same time. Through 
      the combination of calcium carbonate crystal filling and extracellular 
      polysaccharide bonding, the strength and ductility of the sand column were 
      improved, and the brittle characteristics of traditional MICP technology were 
      reduced. By studying the effect of nutrient elements of the culture medium on 
      calcium precipitation and polysaccharide yield, the two metabolites of XR1(#) 
      bacteria could be artificially regulated. In an experiment with 
      microbial-cemented fine sand, compared with the traditional Sporosarcina 
      pasteurii, XR1(#)-cemented sand columns had high production and uniform 
      distribution of calcium carbonate, high strength, and good ductility, and showed 
      obvious advantages. This study provides a new strain with high efficiency and 
      multiple functions for MICP-bonding technology and a new research idea for 
      reducing MICP brittleness.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Wang, Xiaorong
AU  - Wang X
AD  - College of Science, Inner Mongolia University of Technology, Hohhot, China; 
      College of Civil Engineering, Inner Mongolia University of Technology, Hohhot, 
      China; Key Lab. of University of Geological Hazards and Geotechnical Engineering 
      Defense in Sandy, Drought and Cold Regions, Inner Mongolia Autonomous Region, 
      China.
FAU - Li, Chi
AU  - Li C
AD  - College of Civil Engineering, Inner Mongolia University of Technology, Hohhot, 
      China; Key Lab. of University of Geological Hazards and Geotechnical Engineering 
      Defense in Sandy, Drought and Cold Regions, Inner Mongolia Autonomous Region, 
      China. Electronic address: tjdxlch2003@126.com.
FAU - He, Jia
AU  - He J
AD  - Key Laboratory of Ministry of Education for Geomechanics and Embankment 
      Engineering, Hehai University, Nanjing, China.
LA  - eng
PT  - Journal Article
DEP - 20220413
PL  - Netherlands
TA  - J Biotechnol
JT  - Journal of biotechnology
JID - 8411927
RN  - 0 (Sand)
RN  - 0 (Soil)
RN  - H0G9379FGK (Calcium Carbonate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Bacteria/genetics/metabolism
MH  - Calcium
MH  - *Calcium Carbonate/chemistry
MH  - Chemical Precipitation
MH  - *Sand
MH  - Soil/chemistry
MH  - Technology
OTO - NOTNLM
OT  - Bacillus oceanicus
OT  - Biogeotechnical engineering
OT  - Brittleness reduction
OT  - MICP-bonding
OT  - Microbially induced calcite precipitation (MICP)
EDAT- 2022/04/17 06:00
MHDA- 2022/05/14 06:00
CRDT- 2022/04/16 20:09
PHST- 2021/09/14 00:00 [received]
PHST- 2022/03/24 00:00 [revised]
PHST- 2022/03/24 00:00 [accepted]
PHST- 2022/04/17 06:00 [pubmed]
PHST- 2022/05/14 06:00 [medline]
PHST- 2022/04/16 20:09 [entrez]
AID - S0168-1656(22)00074-8 [pii]
AID - 10.1016/j.jbiotec.2022.03.016 [doi]
PST - ppublish
SO  - J Biotechnol. 2022 May 20;350:55-66. doi: 10.1016/j.jbiotec.2022.03.016. Epub 
      2022 Apr 13.