PMID- 35432411
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 1664-462X (Print)
IS  - 1664-462X (Electronic)
IS  - 1664-462X (Linking)
VI  - 13
DP  - 2022
TI  - The Evolution of Cytogenetic Traits in Cuscuta (Convolvulaceae), the Genus With 
      the Most Diverse Chromosomes in Angiosperms.
PG  - 842260
LID - 10.3389/fpls.2022.842260 [doi]
LID - 842260
AB  - Karyotypes are characterized by traits such as chromosome number, which can 
      change through whole-genome duplication and dysploidy. In the parasitic plant 
      genus Cuscuta (Convolvulaceae), chromosome numbers vary more than 18-fold. In 
      addition, species of this group show the highest diversity in terms of genome 
      size among angiosperms, as well as a wide variation in the number and 
      distribution of 5S and 35S ribosomal DNA (rDNA) sites. To understand its 
      karyotypic evolution, ancestral character state reconstructions were performed 
      for chromosome number, genome size, and position of 5S and 35S rDNA sites. 
      Previous cytogenetic data were reviewed and complemented with original chromosome 
      counts, genome size estimates, and rDNA distribution assessed via fluorescence in 
      situ hybridization (FISH), for two, seven, and 10 species, respectively. Starting 
      from an ancestral chromosome number of x = 15, duplications were inferred as the 
      prevalent evolutionary process. However, in holocentric clade (subgenus Cuscuta), 
      dysploidy was identified as the main evolutionary mechanism, typical of 
      holocentric karyotypes. The ancestral genome size of Cuscuta was inferred as 
      approximately 1C = 12 Gbp, with an average genome size of 1C = 2.8 Gbp. This 
      indicates an expansion of the genome size relative to other Convolvulaceae, which 
      may be linked to the parasitic lifestyle of Cuscuta. Finally, the position of 
      rDNA sites varied mostly in species with multiple sites in the same karyotype. 
      This feature may be related to the amplification of rDNA sites in association to 
      other repeats present in the heterochromatin. The data suggest that different 
      mechanisms acted in different subgenera, generating the exceptional diversity of 
      karyotypes in Cuscuta.
CI  - Copyright (c) 2022 Ibiapino, Garcia, Amorim, Baez, Costea, Stefanovic and 
      Pedrosa-Harand.
FAU - Ibiapino, Amalia
AU  - Ibiapino A
AD  - Laboratory of Plant Cytogenetics and Evolution, Department of Botany, Federal 
      University of Pernambuco, Recife, Brazil.
FAU - Garcia, Miguel A
AU  - Garcia MA
AD  - Real Jardin Botanico-CSIC, Madrid, Spain.
FAU - Amorim, Bruno
AU  - Amorim B
AD  - Postgraduate Program of Biotechnology and Natural Resources of the Amazonia 
      (PPGMBT), State University of Amazonas, Manaus, Brazil.
FAU - Baez, Mariana
AU  - Baez M
AD  - Plant Breeding Department, University of Bonn, Bonn, Germany.
FAU - Costea, Mihai
AU  - Costea M
AD  - Department of Biology, University of Wilfrid Laurier, Waterloo, ON, Canada.
FAU - Stefanovic, Sasa
AU  - Stefanovic S
AD  - Department of Biology, University of Toronto Mississauga, Mississauga, ON, 
      Canada.
FAU - Pedrosa-Harand, Andrea
AU  - Pedrosa-Harand A
AD  - Laboratory of Plant Cytogenetics and Evolution, Department of Botany, Federal 
      University of Pernambuco, Recife, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20220401
PL  - Switzerland
TA  - Front Plant Sci
JT  - Frontiers in plant science
JID - 101568200
PMC - PMC9011109
OTO - NOTNLM
OT  - ancestral chromosome number
OT  - character evolution
OT  - genome size
OT  - heterochromatin
OT  - karyotype evolution
OT  - ribosomal DNA
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/04/19 06:00
MHDA- 2022/04/19 06:01
PMCR- 2022/01/01
CRDT- 2022/04/18 06:34
PHST- 2021/12/23 00:00 [received]
PHST- 2022/03/03 00:00 [accepted]
PHST- 2022/04/18 06:34 [entrez]
PHST- 2022/04/19 06:00 [pubmed]
PHST- 2022/04/19 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fpls.2022.842260 [doi]
PST - epublish
SO  - Front Plant Sci. 2022 Apr 1;13:842260. doi: 10.3389/fpls.2022.842260. eCollection 
      2022.