PMID- 35435130
OWN - NLM
STAT- MEDLINE
DCOM- 20220419
LR  - 20220716
IS  - 2165-5987 (Electronic)
IS  - 2165-5979 (Print)
IS  - 2165-5979 (Linking)
VI  - 13
IP  - 4
DP  - 2022 Apr
TI  - Long intergenic non-protein coding RNA 847 promotes laryngeal squamous cell 
      carcinoma progression through the microRNA-181a-5p/zinc finger E-box binding 
      homeobox 2 axis.
PG  - 9987-10000
LID - 10.1080/21655979.2022.2062531 [doi]
AB  - The present study is targeted at investigating the effects of long intergenic 
      non-protein coding RNA 847 (LINC00847) on the malignant biological behaviors of 
      laryngeal squamous cell carcinoma (LSCC) cells, and the mechanisms. Quantitative 
      real-time PCR and Western blotting were conducted for detecting the expressions 
      of LINC00847, microRNA-181a-5p (miR-181a-5p) and zinc finger E-box binding 
      homeobox 2 (ZEB2) in LSCC cell lines and tissue samples. BrdU, cell counting 
      kit-8, scratch wound healing, Transwell and flow cytometry assays were utilized 
      for detecting cell proliferation, migration, invasion, and cell cycle 
      progression. Dual-luciferase reporter gene, RNA binding protein 
      immunoprecipitation (RIP), and RNA pull-down assays were utilized to investigate 
      the interaction among LINC00847, miR-181a-5p, and ZEB2. The subcellular location 
      of LINC00847 was determined by RNA fluorescence in situ hybridization (RNA-FISH) 
      assay. Tumor growth was evaluated using a xenograft model of nude mice. It was 
      revealed that LINC00847 expression was increased in LSCC tissues, and its high 
      expression was associated with lymph node metastasis and poor differentiation. 
      LINC00847 was mainly located in the cytoplasm of LSCC cells, and LINC00847 
      overexpression promoted LSCC cell proliferation, migration, invasion, and 
      accelerated the cell cycle progression while knocking down LINC00847 had the 
      opposite effects in vitro and inhibited the tumor growth in vivo. LINC00847 
      directly targeted miR-181a-5p and negatively modulated miR-181a-5p expression. 
      ZEB2 was a target gene of miR-181a-5p, and was positively and indirectly 
      modulated by LINC00847. Our data suggest that LINC00847 promotes LSCC progression 
      by regulating the miR-181a-5p/ZEB2 axis.
FAU - Li, Wei
AU  - Li W
AD  - Department of Otolaryngology, Ezhou Central Hospital, Hubei, Ezhou Province, P.R. 
      China.
FAU - Hu, Xionghui
AU  - Hu X
AD  - Department of Otolaryngology, Tongxiang First People's Hospital, Jiaxing, 
      Zhejiang Province, P.R. China.
FAU - Huang, Xiaolin
AU  - Huang X
AUID- ORCID: 0000-0002-9084-886X
AD  - Department of Otolaryngology, Ezhou Central Hospital, Hubei, Ezhou Province, P.R. 
      China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Bioengineered
JT  - Bioengineered
JID - 101581063
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (Zinc Finger E-box Binding Homeobox 2)
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - *Head and Neck Neoplasms
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Laryngeal Neoplasms/genetics/metabolism/pathology
MH  - Mice
MH  - Mice, Nude
MH  - *MicroRNAs/genetics/metabolism
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - Squamous Cell Carcinoma of Head and Neck/genetics
MH  - Zinc Finger E-box Binding Homeobox 2/genetics
PMC - PMC9161931
OTO - NOTNLM
OT  - LINC00847
OT  - LSCC
OT  - ZEB2
OT  - miR-181a-5p
OT  - migration and invasion
OT  - proliferation
COIS- No potential conflict of interest was reported by the author(s).
EDAT- 2022/04/19 06:00
MHDA- 2022/04/20 06:00
PMCR- 2022/04/17
CRDT- 2022/04/18 08:40
PHST- 2022/04/18 08:40 [entrez]
PHST- 2022/04/19 06:00 [pubmed]
PHST- 2022/04/20 06:00 [medline]
PHST- 2022/04/17 00:00 [pmc-release]
AID - 2062531 [pii]
AID - 10.1080/21655979.2022.2062531 [doi]
PST - ppublish
SO  - Bioengineered. 2022 Apr;13(4):9987-10000. doi: 10.1080/21655979.2022.2062531.