PMID- 35436273
OWN - NLM
STAT- MEDLINE
DCOM- 20220420
LR  - 20220420
IS  - 1426-9686 (Print)
IS  - 1426-9686 (Linking)
VI  - 50
IP  - 296
DP  - 2022 Apr 19
TI  - Dental status and oxidative homeostasis state in patients exposed to occupational 
      vibration: superoxide dismutase and catalase content in oral fluid.
PG  - 109-113
AB  - AIM: The aim of the research involved determination of catalase and superoxide 
      dismutase content in oral fluid of patients exposed to occupational vibration 
      depending upon their dental status. MATERIALS AND METHODS: The assessment of 
      dental status (DS) and superoxide dismutase (SOD) and catalase (CAT) content in 
      oral fluid (OF) was performed in three groups of patients: control group (n0=129) 
      included the persons exposed to occupational vibration and whose results of 
      combined medical examination excluded the presence of vibration disease (VD); the 
      second (n1=63 patients with VD stage I) and the third (n1=66 patients with VD 
      stage II) groups consisted of the patients, who underwent treatment at the 
      clinical department of the Research Institute of Occupational Hygiene and 
      Occupational Diseases of Kharkiv National Medical University of the Ministry of 
      Health of Ukraine. DS determination was carried out according to the method of K. 
      M. Kosenko (Patent No. 57512, Ukraine) for in-patients and controls (during 
      medical checkups) using the following indices: PMA, OHI-S, DMFT, with assessment 
      of vacuum-pressory resistance of gingival capillaries (VPRC) (according to V. I. 
      Kulazhenko) and community periodontal index of treatment needs (CPITN). SOD 
      content was determined by the nonenzymatic method; CAT content was revealed 
      spectrophotometrically. Primary data were statistically processed with the 
      determination of accuracy by Student's test. RESULTS: SOD content depending upon 
      PMA intensity in VD patients ranged from 14.1+/-0.2 U/min to 15.7+/-0.5 U/min , was 
      reliably (p<0.05) lower in patients with VD versus the controls (17.8+/-0.2 U/min 
      and 14.2+/-0.2 U/min respectively, when PMA>2.1) and did not differ depending upon 
      VD severity (15.7+/-0.5 U/min in VD stage I and 15.3+/-0.3 U/min in VD stage II, 
      respectively). SOD content in OF in patients depending upon their OHI-S ranged 
      from 13.5+/-0.3 U/min to 16.3+/-0.2 U/min and was reliably (p<0.05) lower in patients 
      with OHI-S>/=1.7 U. A comparative analysis showed that the activity of the 
      enzymatic protection of the periodontal membrane could be also determined by the 
      state of hard tissues, in particular by such DS index as DFTM. The activity of 
      SOD in VD stage II was found to be reliably (p<0.05) reduced in patients with 
      DFTM index exceeding 15 pts. A somewhat different pattern of SOD activity was 
      found in OF in patients with VD stage I: SOD activity in OF was similar in all 
      DFTM indices and it became reduced depending upon an increase of DFTM index. SOD 
      content depending upon VPRC index in patients with VD ranged from 10.7+/-0.5 U/min 
      to 16.8+/-0.3 U/min and was reliably (p<0.05) lower in cases with VPRC index </=40 
      sec. CAT content depending upon PMA intensity in VD patients ranged from 4.6+/-0.4 
      U/min to 11.3+/-0.3 U/min and was reliably (p<0.05) higher in patients with VD 
      stage I versus the controls and differed according to the severity. CAT content 
      in OF in patients depending upon OHI-S ranged from 5.2+/-0.2 U/min to 10.1+/-0.3 
      U/min, was reliably (p<0.05) lower in cases with OHI-S >/=1.7 U, did not differ 
      from the indices observed in the control group and was also found to be reliably 
      lower in patients with VD stage II versus those with VD stage I (7.3+/-0.3 U/min 
      and 8.6+/-0.2 U/min, respectively, when OHI-S ranged within 0.7/1.6 U). CAT content 
      in OF depending upon VPSC index in patients with VD ranged from 5.8+/-0.2 U/min to 
      8.6+/-0.6 U/min and was reliably (p<0.05) lower in cases with VPSC index </=40 sec. 
      Thus, CAT activity in OF in patients was reliably (p<0.05) reduced (in VPRC>40 
      sec it was equal to 7.8+/-0.2 U/min, and in VPRC</=40 sec it was 8.6+/-0.1 U/ min) in 
      VD stage I with decreased VPRC. CONCLUSIONS: A trend (p>0.05) towards an increase 
      in SOD activity in VD stage I versus the controls was revealed, whereas VD stage 
      II demonstrated a reliable (p<0.05) reduction of the above activity. At the same 
      time, an unsatisfactory state of oral hygiene was shown to promote inhibition of 
      the enzymatic protection of their periodontal membrane in patients with VD stage 
      I. A trend (p>0.05) towards an increase of SOD activity in VD stage I versus the 
      controls was revealed, whereas VD stage II demonstrated a reliable (p<0.05) 
      reduction of the above activity. The assessment carried out in cases requiring 
      combined treatment with surgical or non-surgical debridement and also in patients 
      with supraor subgingival dental calculus found out that SOD activity was reliably 
      reduced only in cases with VD stage II. CAT activity assessment in OF in VD 
      patients having different levels of CPITN showed that the above activity in 
      persons requiring combined treatment (including prosthodontic treatment; 
      CPITN>/=3.1points) was markedly and reliably reduced. All the above facts determine 
      peculiarities in oral treatment strategies for this group of patients.
CI  - (c) 2022 MEDPRESS.
FAU - Cherkashyna, Lidiya
AU  - Cherkashyna L
AD  - Kharkiv Medical Academy of Postgraduate Education, Ukraine.
FAU - Haidash, Iryna
AU  - Haidash I
AD  - Lugansk State Medical University, Ukraine.
FAU - Hlazkova, Natalya
AU  - Hlazkova N
AD  - Lugansk State Medical University, Ukraine.
FAU - Demikhova, Nadiia
AU  - Demikhova N
AD  - Sumy State University, Ukraine; 4Kharkiv National Medical University, Ukraine.
FAU - Shklyar, Anton
AU  - Shklyar A
AD  - Kharkiv National Medical University, Ukraine.
FAU - Sukhonosov, Roman
AU  - Sukhonosov R
AD  - Kharkiv National Medical University, Ukraine.
FAU - Barchan, Ganna
AU  - Barchan G
AD  - Kharkiv National Medical University, Ukraine.
FAU - Kuts, Larysa
AU  - Kuts L
AD  - Sumy State University, Ukraine.
FAU - Demikhov, Andrii
AU  - Demikhov A
AD  - Sumy State University, Ukraine.
LA  - eng
PT  - Journal Article
PL  - Poland
TA  - Pol Merkur Lekarski
JT  - Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego
JID - 9705469
RN  - 0 (Antioxidants)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Antioxidants
MH  - Catalase
MH  - Homeostasis
MH  - Humans
MH  - Oxidative Stress
MH  - *Superoxide Dismutase
MH  - *Vibration
OTO - NOTNLM
OT  - dental status
OT  - periodontal membrane
OT  - vibration disease
EDAT- 2022/04/19 06:00
MHDA- 2022/04/21 06:00
CRDT- 2022/04/18 17:10
PHST- 2022/04/18 17:10 [entrez]
PHST- 2022/04/19 06:00 [pubmed]
PHST- 2022/04/21 06:00 [medline]
AID - PML296-109 [pii]
PST - ppublish
SO  - Pol Merkur Lekarski. 2022 Apr 19;50(296):109-113.