PMID- 35436733
OWN - NLM
STAT- MEDLINE
DCOM- 20220530
LR  - 20220530
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 245
DP  - 2022 Aug 1
TI  - Pump-free microfluidic chip based laryngeal squamous cell carcinoma-related 
      microRNAs detection through the combination of surface-enhanced Raman scattering 
      techniques and catalytic hairpin assembly amplification.
PG  - 123478
LID - S0039-9140(22)00274-0 [pii]
LID - 10.1016/j.talanta.2022.123478 [doi]
AB  - MicroRNA (miRNA), as one of the ideal target biomarker analytes, plays an 
      essential role in biological processes; thus, the development of rapidly 
      sensitive detection methods is imperative. Herein, we proposed a pump-free 
      surface-enhanced Raman scatting (SERS) microfluidic chip for the rapid and 
      ultrasensitive detection of miR-106b and miR-196b, laryngeal squamous cell 
      carcinoma (LSCC)-related miRNAs. Ag-Au core-shell nanorods (Ag-AuNRs) were 
      applied to prepare SERS tags by modifying Raman reporters and hairpin DNAs. The 
      capture probes were synthesized by labeling hairpin DNAs onto the magnetic beads 
      (MBs) surface. In the presence of targets, the catalytic hairpin assembly (CHA) 
      reactions between SERS tags and capture probes could be triggered, causing the 
      aggregation of Ag-AuNRs. The tiny magnets installed under the rectangular chamber 
      could magnetically gather the CHA products, leading to the further aggregation of 
      Ag-AuNRs. Thus, this strategy could achieve the double aggregation of Ag-AuNRs, 
      resulting in the significant amplification of the SERS signal. The proposed 
      strategy achieved simultaneous and sensitive detection of miR-106b and miR-196b, 
      with limits of detection low to aM level. The whole detection process could be 
      completed within 5 min. Moreover, this microfluidic chip exhibited excellent 
      reproducibility, stability, and specificity. The high accuracy of this SERS 
      microfluidic chip was proved by practical analysis in LSCC patients' serum. The 
      results demonstrated that SERS could be a promising alternative clinical 
      diagnosis tool and exhibited potential application for the dynamic monitoring of 
      cancer staging.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Ge, Shengjie
AU  - Ge S
AD  - Institute of Translational Medicine, Medical College, Yangzhou University, 
      Yangzhou, 225001, PR China; Jiangsu Key Laboratory of Integrated Traditional 
      Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, 
      Yangzhou University, Yangzhou, PR China; Jiangsu Key Laboratory of Experimental & 
      Translational Noncoding RNA Research, Medical College, Yangzhou University, 
      Yangzhou, China.
FAU - Li, Guang
AU  - Li G
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, The Affiliated Hospital 
      of Yangzhou University, Yangzhou University, Yangzhou, 225001, PR China.
FAU - Zhou, Xinyu
AU  - Zhou X
AD  - Institute of Translational Medicine, Medical College, Yangzhou University, 
      Yangzhou, 225001, PR China; Jiangsu Key Laboratory of Integrated Traditional 
      Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, 
      Yangzhou University, Yangzhou, PR China; Jiangsu Key Laboratory of Experimental & 
      Translational Noncoding RNA Research, Medical College, Yangzhou University, 
      Yangzhou, China.
FAU - Mao, Yu
AU  - Mao Y
AD  - Institute of Translational Medicine, Medical College, Yangzhou University, 
      Yangzhou, 225001, PR China; Jiangsu Key Laboratory of Integrated Traditional 
      Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, 
      Yangzhou University, Yangzhou, PR China; Jiangsu Key Laboratory of Experimental & 
      Translational Noncoding RNA Research, Medical College, Yangzhou University, 
      Yangzhou, China.
FAU - Gu, Yingyan
AU  - Gu Y
AD  - Institute of Translational Medicine, Medical College, Yangzhou University, 
      Yangzhou, 225001, PR China; Jiangsu Key Laboratory of Integrated Traditional 
      Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, 
      Yangzhou University, Yangzhou, PR China; Jiangsu Key Laboratory of Experimental & 
      Translational Noncoding RNA Research, Medical College, Yangzhou University, 
      Yangzhou, China.
FAU - Li, Zhiyue
AU  - Li Z
AD  - The First Clinical College, Dalian Medical University, Dalian, PR China.
FAU - Gu, Yuexing
AU  - Gu Y
AD  - Institute of Translational Medicine, Medical College, Yangzhou University, 
      Yangzhou, 225001, PR China; Jiangsu Key Laboratory of Integrated Traditional 
      Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, 
      Yangzhou University, Yangzhou, PR China; Jiangsu Key Laboratory of Experimental & 
      Translational Noncoding RNA Research, Medical College, Yangzhou University, 
      Yangzhou, China.
FAU - Cao, Xiaowei
AU  - Cao X
AD  - Institute of Translational Medicine, Medical College, Yangzhou University, 
      Yangzhou, 225001, PR China; Jiangsu Key Laboratory of Integrated Traditional 
      Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, 
      Yangzhou University, Yangzhou, PR China; Jiangsu Key Laboratory of Experimental & 
      Translational Noncoding RNA Research, Medical College, Yangzhou University, 
      Yangzhou, China. Electronic address: cxw19861121@163.com.
LA  - eng
PT  - Journal Article
DEP - 20220412
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (MicroRNAs)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - *Biosensing Techniques/methods
MH  - Gold
MH  - *Head and Neck Neoplasms
MH  - Humans
MH  - Limit of Detection
MH  - *Metal Nanoparticles
MH  - *MicroRNAs/analysis
MH  - Microfluidics
MH  - Reproducibility of Results
MH  - Spectrum Analysis, Raman/methods
MH  - Squamous Cell Carcinoma of Head and Neck
OTO - NOTNLM
OT  - Catalytic hairpin assembly
OT  - Laryngeal squamous cell carcinoma
OT  - Microfluidic chip
OT  - Surface-enhanced Raman scattering
OT  - miRNA
EDAT- 2022/04/19 06:00
MHDA- 2022/05/31 06:00
CRDT- 2022/04/18 20:09
PHST- 2021/12/27 00:00 [received]
PHST- 2022/04/07 00:00 [revised]
PHST- 2022/04/10 00:00 [accepted]
PHST- 2022/04/19 06:00 [pubmed]
PHST- 2022/05/31 06:00 [medline]
PHST- 2022/04/18 20:09 [entrez]
AID - S0039-9140(22)00274-0 [pii]
AID - 10.1016/j.talanta.2022.123478 [doi]
PST - ppublish
SO  - Talanta. 2022 Aug 1;245:123478. doi: 10.1016/j.talanta.2022.123478. Epub 2022 Apr 
      12.