PMID- 35436853
OWN - NLM
STAT- MEDLINE
DCOM- 20220420
LR  - 20220716
IS  - 1471-2164 (Electronic)
IS  - 1471-2164 (Linking)
VI  - 23
IP  - 1
DP  - 2022 Apr 18
TI  - Development and application of specific FISH probes for karyotyping 
      Psathyrostachys huashanica chromosomes.
PG  - 309
LID - 10.1186/s12864-022-08516-6 [doi]
LID - 309
AB  - BACKGROUND: Psathyrostachys huashanica Keng has long been used as a genetic 
      resource for improving wheat cultivar because of its genes mediating the 
      resistance to various diseases (stripe rust, leaf rust, take-all, and powdery 
      mildew) as well as its desirable agronomic traits. However, a high-resolution 
      fluorescence in situ hybridization (FISH) karyotype of P. huashanica remains 
      unavailable. RESULTS: To develop chromosome-specific FISH markers for P. 
      huashanica, repetitive sequences, including pSc119.2, pTa535, pTa713, pAs1, 
      (AAC)(5), (CTT)(12), pSc200, pTa71A-2, and Oligo-44 were used for a FISH 
      analysis. The results indicated that the combination of pSc200, pTa71A-2 and 
      Oligo-44 probes can clearly identify all Ns genomic chromosomes in the two P. 
      huashanica germplasms. The homoeologous relationships between individual P. 
      huashanica chromosomes and common wheat chromosomes were clarified by FISH 
      painting. Marker validation analyses revealed that the combination of pSc200, 
      pTa71A-2, and Oligo-44 for a FISH analysis can distinguish the P. huashanica 
      Ns-genome chromosomes from wheat chromosomes, as well as all chromosomes (except 
      4Ns) from the chromosomes of diploid wheat relatives carrying St, E, V, I, P and 
      R genomes. Additionally, the probes were applicable for discriminating between 
      the P. huashanica Ns-genome chromosomes in all homologous groups and the 
      corresponding chromosomes in Psathyrostachys juncea and most Leymus species 
      containing the Ns genome. Furthermore, six wheat-P. huashanica chromosome 
      addition lines (i.e., 2Ns, 3Ns, 4Ns, 7Ns chromosomes and chromosomal segments) 
      were characterized using the newly developed FISH markers. Thus, these probes can 
      rapidly and precisely detect P. huashanica alien chromosomes in the wheat 
      background. CONCLUSIONS: The FISH karyotype established in this study lays a 
      solid foundation for the efficient identification of P. huashanica chromosomes in 
      wheat genetic improvement programs.
CI  - (c) 2022. The Author(s).
FAU - Zhang, Hao
AU  - Zhang H
AD  - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, 
      Sichuan Agricultural University, 611130, Chengdu, Sichuan, China.
AD  - Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, 
      Sichuan, China.
FAU - Wang, Fei
AU  - Wang F
AD  - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, 
      Sichuan Agricultural University, 611130, Chengdu, Sichuan, China.
AD  - Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, 
      Sichuan, China.
FAU - Zeng, Chunyan
AU  - Zeng C
AD  - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, 
      Sichuan Agricultural University, 611130, Chengdu, Sichuan, China.
AD  - Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, 
      Sichuan, China.
FAU - Zhu, Wei
AU  - Zhu W
AD  - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, 
      Sichuan Agricultural University, 611130, Chengdu, Sichuan, China.
AD  - Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, 
      Sichuan, China.
FAU - Xu, Lili
AU  - Xu L
AD  - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, 
      Sichuan Agricultural University, 611130, Chengdu, Sichuan, China.
FAU - Wang, Yi
AU  - Wang Y
AD  - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, 
      Sichuan Agricultural University, 611130, Chengdu, Sichuan, China.
AD  - Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, 
      Sichuan, China.
FAU - Zeng, Jian
AU  - Zeng J
AD  - College of Resources, Sichuan Agricultural University, 611130, Chengdu, Sichuan, 
      China.
FAU - Fan, Xing
AU  - Fan X
AD  - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, 
      Sichuan Agricultural University, 611130, Chengdu, Sichuan, China.
AD  - Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, 
      Sichuan, China.
FAU - Sha, Lina
AU  - Sha L
AD  - College of Grassland Science and Technology, Sichuan Agricultural University, 
      611130, Chengdu, Sichuan, China.
FAU - Wu, Dandan
AU  - Wu D
AD  - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, 
      Sichuan Agricultural University, 611130, Chengdu, Sichuan, China.
AD  - Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, 
      Sichuan, China.
FAU - Cheng, Yiran
AU  - Cheng Y
AD  - Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, 
      Sichuan, China.
FAU - Zhang, Haiqin
AU  - Zhang H
AD  - College of Grassland Science and Technology, Sichuan Agricultural University, 
      611130, Chengdu, Sichuan, China.
FAU - Chen, Guoyue
AU  - Chen G
AD  - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, 
      Sichuan Agricultural University, 611130, Chengdu, Sichuan, China.
AD  - Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, 
      Sichuan, China.
FAU - Zhou, Yonghong
AU  - Zhou Y
AD  - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, 
      Sichuan Agricultural University, 611130, Chengdu, Sichuan, China.
AD  - Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, 
      Sichuan, China.
FAU - Kang, Houyang
AU  - Kang H
AD  - State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, 
      Sichuan Agricultural University, 611130, Chengdu, Sichuan, China. 
      houyang.kang@sicau.edu.cn.
AD  - Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, 
      Sichuan, China. houyang.kang@sicau.edu.cn.
LA  - eng
GR  - No. 31771781, 31971883/National Natural Science Foundation of China/
GR  - No. 31771781, 31971883/National Natural Science Foundation of China/
GR  - No. 31771781, 31971883/National Natural Science Foundation of China/
GR  - No. 31771781, 31971883/National Natural Science Foundation of China/
GR  - No. 31771781, 31971883/National Natural Science Foundation of China/
GR  - No. 31771781, 31971883/National Natural Science Foundation of China/
GR  - No. 31771781, 31971883/National Natural Science Foundation of China/
GR  - No. 31771781, 31971883/National Natural Science Foundation of China/
GR  - No. 31771781, 31971883/National Natural Science Foundation of China/
GR  - No. 31771781, 31971883/National Natural Science Foundation of China/
GR  - No. 31771781, 31971883/National Natural Science Foundation of China/
GR  - No. 31771781, 31971883/National Natural Science Foundation of China/
GR  - No. 31771781, 31971883/National Natural Science Foundation of China/
GR  - No. 31771781, 31971883/National Natural Science Foundation of China/
GR  - No. 31771781, 31971883/National Natural Science Foundation of China/
GR  - 2020YJ0348/Applied Basic Research Programs of the Science and Technology Bureau 
      of Sichuan Province/
GR  - 2020YJ0348/Applied Basic Research Programs of the Science and Technology Bureau 
      of Sichuan Province/
GR  - 2020YJ0348/Applied Basic Research Programs of the Science and Technology Bureau 
      of Sichuan Province/
GR  - 2020YJ0348/Applied Basic Research Programs of the Science and Technology Bureau 
      of Sichuan Province/
GR  - 2020YJ0348/Applied Basic Research Programs of the Science and Technology Bureau 
      of Sichuan Province/
GR  - 2020YJ0348/Applied Basic Research Programs of the Science and Technology Bureau 
      of Sichuan Province/
GR  - 2020YJ0348/Applied Basic Research Programs of the Science and Technology Bureau 
      of Sichuan Province/
GR  - 2020YJ0348/Applied Basic Research Programs of the Science and Technology Bureau 
      of Sichuan Province/
GR  - 2020YJ0348/Applied Basic Research Programs of the Science and Technology Bureau 
      of Sichuan Province/
GR  - 2020YJ0348/Applied Basic Research Programs of the Science and Technology Bureau 
      of Sichuan Province/
GR  - 2020YJ0348/Applied Basic Research Programs of the Science and Technology Bureau 
      of Sichuan Province/
GR  - 2020YJ0348/Applied Basic Research Programs of the Science and Technology Bureau 
      of Sichuan Province/
GR  - 2020YJ0348/Applied Basic Research Programs of the Science and Technology Bureau 
      of Sichuan Province/
GR  - 2020YJ0348/Applied Basic Research Programs of the Science and Technology Bureau 
      of Sichuan Province/
GR  - 2020YJ0348/Applied Basic Research Programs of the Science and Technology Bureau 
      of Sichuan Province/
GR  - 2021-YF05-00681-SN/Science and Technology Bureau of Chengdu City/
GR  - 2021-YF05-00681-SN/Science and Technology Bureau of Chengdu City/
GR  - 2021-YF05-00681-SN/Science and Technology Bureau of Chengdu City/
GR  - 2021-YF05-00681-SN/Science and Technology Bureau of Chengdu City/
GR  - 2021-YF05-00681-SN/Science and Technology Bureau of Chengdu City/
GR  - 2021-YF05-00681-SN/Science and Technology Bureau of Chengdu City/
GR  - 2021-YF05-00681-SN/Science and Technology Bureau of Chengdu City/
GR  - 2021-YF05-00681-SN/Science and Technology Bureau of Chengdu City/
GR  - 2021-YF05-00681-SN/Science and Technology Bureau of Chengdu City/
GR  - 2021-YF05-00681-SN/Science and Technology Bureau of Chengdu City/
GR  - 2021-YF05-00681-SN/Science and Technology Bureau of Chengdu City/
GR  - 2021-YF05-00681-SN/Science and Technology Bureau of Chengdu City/
GR  - 2021-YF05-00681-SN/Science and Technology Bureau of Chengdu City/
GR  - 2021-YF05-00681-SN/Science and Technology Bureau of Chengdu City/
GR  - 2021-YF05-00681-SN/Science and Technology Bureau of Chengdu City/
PT  - Journal Article
DEP - 20220418
PL  - England
TA  - BMC Genomics
JT  - BMC genomics
JID - 100965258
RN  - 0 (Genetic Markers)
SB  - IM
MH  - *Chromosomes, Plant/genetics
MH  - *Disease Resistance/genetics
MH  - Genetic Markers
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Plant Diseases/genetics
MH  - Poaceae/genetics
MH  - Triticum/genetics
PMC - PMC9017042
OTO - NOTNLM
OT  - Addition lines
OT  - Chromosome identification
OT  - FISH painting
OT  - Fluorescence in situ hybridization karyotype
OT  - Psathyrostachys huashanica
COIS- All authors declare that there is no conflict of interest.
EDAT- 2022/04/20 06:00
MHDA- 2022/04/21 06:00
PMCR- 2022/04/18
CRDT- 2022/04/19 05:04
PHST- 2021/12/11 00:00 [received]
PHST- 2022/03/24 00:00 [accepted]
PHST- 2022/04/19 05:04 [entrez]
PHST- 2022/04/20 06:00 [pubmed]
PHST- 2022/04/21 06:00 [medline]
PHST- 2022/04/18 00:00 [pmc-release]
AID - 10.1186/s12864-022-08516-6 [pii]
AID - 8516 [pii]
AID - 10.1186/s12864-022-08516-6 [doi]
PST - epublish
SO  - BMC Genomics. 2022 Apr 18;23(1):309. doi: 10.1186/s12864-022-08516-6.