PMID- 35437459
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220503
IS  - 1993-0771 (Print)
IS  - 1993-078X (Electronic)
IS  - 1993-0771 (Linking)
VI  - 16
IP  - 1
DP  - 2022
TI  - Karyotypic analysis and isolation of four DNA markers of the scleractinian coral 
      Favitespentagona (Esper, 1795) (Scleractinia, Anthozoa, Cnidaria).
PG  - 77-92
LID - 10.3897/compcytogen.v16.i1.79953 [doi]
AB  - We performed conventional and molecular cytogenetic studies on the 
      Favitespentagona Esper, 1795, a scleractinian coral mostly found along the west 
      coast of Japan. Karyotype analysis of F.pentagona by G-banding revealed a 
      karyogram containing a homogenously staining region (HSR) on chromosome 10 in 
      more than 50% of the examined metaphase spreads. This HSR consisted of sequences 
      from 18S ribosomal RNA (rRNA) genes, as demonstrated by fluorescence in situ 
      hybridization (FISH) and DNA sequencing. We highlighted the development of four 
      chromosomal FISH markers from repetitive genes such as U2 small nuclear RNA 
      linked to 5S rRNA sequence (U2 snRNA-5S), 18S rRNA, histone H3, and 
      uncharacterized gene FP-9X. The chromosomal locations of the U2 snRNA-5S and 18S 
      RNA were on the terminal end of long arm of chromosomes 2 and 10, respectively, 
      while the histone H3 and the uncharacterized gene were located near the 
      centromeres of chromosomes 1 and 9, respectively. These FISH markers will improve 
      the karyotyping of F.pentagona from mitotic preparations which helps in widening 
      our understanding of coral genetic structure and chromosome organization. In 
      addition, these improvements in karyotyping will provide the basis in 
      constructing of chromosome-level genome assembly for F.pentagona.
CI  - Rei Kawakami, Takahiro Taguchi, Joshua Vacarizas, Masumi Ito, Takuma Mezaki, 
      Akira Tominaga, Satoshi Kubota.
FAU - Kawakami, Rei
AU  - Kawakami R
AD  - Agriculture and Marine Science Program, Graduate School of Integrated Arts and 
      Sciences, Kochi University, Kohasu, Oko-Cho, Nankoku, Kochi 783-8505, Japan.
FAU - Taguchi, Takahiro
AU  - Taguchi T
AUID- ORCID: 0000-0002-9095-2456
AD  - Department of Nutrition, Faculty of Health Science, Kochi Gakuen University, 
      292-26 Asahitenjin-Cho, Kochi 780-0955, Japan.
AD  - Kuroshio Science Unit, Multidisciplinary Science Cluster, Kochi University, 
      Kohasu, Oko-Cho, Nankoku, Kochi 783-8505, Japan.
FAU - Vacarizas, Joshua
AU  - Vacarizas J
AD  - Kuroshio Science Program, Graduate School of Integrated Arts and Sciences, Kochi 
      University, Kohasu, Oko-Cho, Nankoku, Kochi 783-8505, Japan.
FAU - Ito, Masumi
AU  - Ito M
AD  - Faculty of Agriculture and Marine Science, Kochi University, 200 Otsu, Monobe, 
      Kochi 783-8502, Japan.
AD  - Graduate School of Fisheries Sciences, Hokkaido University, 3-1-1 Minato-Cho, 
      Hakodate, Hokkaido, 041-8611, Japan.
FAU - Mezaki, Takuma
AU  - Mezaki T
AD  - Kuroshio Biological Research Foundation, Otsuki, Hata County, Kochi 788-0333, 
      Japan.
FAU - Tominaga, Akira
AU  - Tominaga A
AD  - Kuroshio Science Program, Graduate School of Integrated Arts and Sciences, Kochi 
      University, Kohasu, Oko-Cho, Nankoku, Kochi 783-8505, Japan.
FAU - Kubota, Satoshi
AU  - Kubota S
AD  - Kuroshio Science Unit, Multidisciplinary Science Cluster, Kochi University, 
      Kohasu, Oko-Cho, Nankoku, Kochi 783-8505, Japan.
LA  - eng
PT  - Journal Article
DEP - 20220405
PL  - Bulgaria
TA  - Comp Cytogenet
JT  - Comparative cytogenetics
JID - 101503807
PMC - PMC9005458
OTO - NOTNLM
OT  - FISH
OT  - HSR
OT  - chromosome
OT  - histone
OT  - karyotype
OT  - rRNA
OT  - scleractinian coral
EDAT- 2022/04/20 06:00
MHDA- 2022/04/20 06:01
PMCR- 2022/04/05
CRDT- 2022/04/19 06:00
PHST- 2021/12/30 00:00 [received]
PHST- 2022/03/05 00:00 [accepted]
PHST- 2022/04/19 06:00 [entrez]
PHST- 2022/04/20 06:00 [pubmed]
PHST- 2022/04/20 06:01 [medline]
PHST- 2022/04/05 00:00 [pmc-release]
AID - 79953 [pii]
AID - 10.3897/compcytogen.v16.i1.79953 [doi]
PST - epublish
SO  - Comp Cytogenet. 2022 Apr 5;16(1):77-92. doi: 10.3897/compcytogen.v16.i1.79953. 
      eCollection 2022.