PMID- 35437949
OWN - NLM
STAT- MEDLINE
DCOM- 20220620
LR  - 20220620
IS  - 1526-4998 (Electronic)
IS  - 1526-498X (Linking)
VI  - 78
IP  - 7
DP  - 2022 Jul
TI  - Penetrance during Wolbachia-mediated parthenogenesis of Trichogramma wasps is 
      reduced by continuous oviposition, associated with exhaustion of Wolbachia titers 
      in ovary and offspring eggs.
PG  - 3080-3089
LID - 10.1002/ps.6934 [doi]
AB  - BACKGROUND: Thelytokous Wolbachia-infected Trichogramma wasps are superior to 
      bisexual uninfected wasps regarding biological control programs. However, 
      continuous oviposition weakens the parthenogenesis-inducing (PI) strength of 
      Wolbachia. Whether this reduced PI strength relates to decreases in the titer of 
      Wolbachia in the ovary and offspring eggs of Trichogramma remains unclear. Here, 
      using fluorescence in situ hybridization (FISH) and reverse transcription 
      quantitative polymerase chain reaction (RT-qPCR) methods, we investigated how the 
      penetrance of Wolbachia-mediated parthenogenesis, Wolbachia density, and 
      distributions of two Wolbachia-infected Trichogramma species, T. pretiosum (TP) 
      and T. dendrolimi (TD), were influenced by different host access treatments 
      [newly-emerged virgin females (NE), 7-day-old females without access to host eggs 
      (NAH), and 7-day-old virgin females with access to host eggs (AH)]. RESULTS: 
      Continuous oviposition decreased Wolbachia PI strength and titers in TP and TD. 
      Continuous oviposition in AH decreased Wolbachia titers in abdomen and offspring 
      eggs of TP and TD females, compared with NAH and NE; NAH had a lower thorax 
      Wolbachia titer than NE. The numbers of parasitized host eggs and offspring 
      wasps, and emergence rates of offspring deposited by AH were lower than those of 
      NE and NAH, for either species. CONCLUSION: Weakened PI strength, driven by 
      continuous oviposition in Trichogramma wasps, is associated with Wolbachia titer 
      exhaustion in ovary and offspring eggs. Wolbachia density is dependent on PI 
      strength in Trichogramma wasps, highlighting the side effects of continuous 
      oviposition regarding thelytokous Wolbachia-infected Trichogramma in biological 
      control programs. (c) 2022 Society of Chemical Industry.
CI  - (c) 2022 Society of Chemical Industry.
FAU - Zhou, Jin-Cheng
AU  - Zhou JC
AUID- ORCID: 0000-0002-5904-0387
AD  - College of Plant Protection, Shenyang Agricultural University, Shenyang, P. R. 
      China.
AD  - Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 
      P. R. China.
FAU - Shang, Dan
AU  - Shang D
AD  - College of Plant Protection, Shenyang Agricultural University, Shenyang, P. R. 
      China.
FAU - Qian, Qian
AU  - Qian Q
AD  - College of Plant Protection, Shenyang Agricultural University, Shenyang, P. R. 
      China.
FAU - Zhang, Chen
AU  - Zhang C
AD  - College of Plant Protection, Shenyang Agricultural University, Shenyang, P. R. 
      China.
FAU - Zhang, Li-Sheng
AU  - Zhang LS
AUID- ORCID: 0000-0001-9578-0094
AD  - Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 
      P. R. China.
FAU - Dong, Hui
AU  - Dong H
AUID- ORCID: 0000-0002-0084-8699
AD  - College of Plant Protection, Shenyang Agricultural University, Shenyang, P. R. 
      China.
LA  - eng
GR  - Agricultural Science and Technology Innovation Program (CAAS-ZDRW202108)/
GR  - Fundamental Research Funds for the Universities of Liaoning Province (LR2019061, 
      LJKZ0646)/
GR  - Major Projects of China National Tobacco Corporation (110202001032 (LS-01))/
GR  - Natural Science Foundation of Liaoning Province (2020-BS-137)/
GR  - Projects of Guizhou Tobacco Corporation (201936, 201937, and 201941)/
GR  - Projects of National Natural Science Foundation of China (32102275, 32172476)/
GR  - Natural Science Foundation of Liaoning Province/
GR  - Agricultural Science and Technology Innovation Program/
GR  - China National Tobacco Corporation/
GR  - National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20220503
PL  - England
TA  - Pest Manag Sci
JT  - Pest management science
JID - 100898744
SB  - IM
MH  - Animals
MH  - Female
MH  - In Situ Hybridization, Fluorescence
MH  - Ovary
MH  - Oviposition
MH  - Parthenogenesis
MH  - Penetrance
MH  - *Wasps/genetics
MH  - *Wolbachia/genetics
OTO - NOTNLM
OT  - biological control
OT  - egg parasitoid
OT  - endosymbiont
OT  - reproduction
OT  - thelytokous parthenogenesis
EDAT- 2022/04/20 06:00
MHDA- 2022/06/22 06:00
CRDT- 2022/04/19 06:04
PHST- 2022/04/11 00:00 [revised]
PHST- 2022/01/04 00:00 [received]
PHST- 2022/04/18 00:00 [accepted]
PHST- 2022/04/20 06:00 [pubmed]
PHST- 2022/06/22 06:00 [medline]
PHST- 2022/04/19 06:04 [entrez]
AID - 10.1002/ps.6934 [doi]
PST - ppublish
SO  - Pest Manag Sci. 2022 Jul;78(7):3080-3089. doi: 10.1002/ps.6934. Epub 2022 May 3.