PMID- 35444958
OWN - NLM
STAT- MEDLINE
DCOM- 20220422
LR  - 20220716
IS  - 2235-2988 (Electronic)
IS  - 2235-2988 (Linking)
VI  - 12
DP  - 2022
TI  - Monitoring Autophagy at Cellular and Molecular Level in Crassostrea gigas During 
      an Experimental Ostreid Herpesvirus 1 (OsHV-1) Infection.
PG  - 858311
LID - 10.3389/fcimb.2022.858311 [doi]
LID - 858311
AB  - Mortality outbreaks of young Pacific oysters, Crassostrea gigas, have seriously 
      affected the oyster-farming economy in several countries around the world. 
      Although the causes of these mortality outbreaks appear complex, a viral agent 
      has been identified as the main factor: a herpesvirus called ostreid herpesvirus 
      1 (OsHV-1). Autophagy is an important degradation pathway involved in the 
      response to several pathologies including viral diseases. In C. gigas, recent 
      studies indicate that this pathway is conserved and functional in at least 
      haemocytes and the mantle. Furthermore, an experimental infection in combination 
      with compounds known to inhibit or induce autophagy in mammals revealed that 
      autophagy is involved in the response to OsHV-1 infection. In light of these 
      results, the aim of this study was to determine the role of autophagy in the 
      response of the Pacific oyster to infection by virus OsHV-1. For this purpose, an 
      experimental infection in combination with a modulator of autophagy was performed 
      on Pacific oysters known to have intermediate susceptibility to OsHV-1 infection. 
      In haemolymph and the mantle, the autophagy response was monitored by flow 
      cytometry, western blotting, and real-time PCR. At the same time, viral infection 
      was evaluated by quantifying viral DNA and RNA amounts by real-time PCR. Although 
      the results showed activation of autophagy in haemolymph and the mantle 14 hours 
      post infection (after viral replication was initiated), they were also indicative 
      of different regulatory mechanisms of autophagy in the two tissues, thus 
      supporting an important function of autophagy in the response to virus OsHV-1.
CI  - Copyright (c) 2022 Picot, Faury, Pelletier, Arzul, Chollet, Degremont, Renault and 
      Morga.
FAU - Picot, Sandy
AU  - Picot S
AD  - Ifremer, ASIM, Adaptation Sante des invertebres, La Tremblade, France.
FAU - Faury, Nicole
AU  - Faury N
AD  - Ifremer, ASIM, Adaptation Sante des invertebres, La Tremblade, France.
FAU - Pelletier, Camille
AU  - Pelletier C
AD  - Ifremer, ASIM, Adaptation Sante des invertebres, La Tremblade, France.
FAU - Arzul, Isabelle
AU  - Arzul I
AD  - Ifremer, ASIM, Adaptation Sante des invertebres, La Tremblade, France.
FAU - Chollet, Bruno
AU  - Chollet B
AD  - Ifremer, ASIM, Adaptation Sante des invertebres, La Tremblade, France.
FAU - Degremont, Lionel
AU  - Degremont L
AD  - Ifremer, ASIM, Adaptation Sante des invertebres, La Tremblade, France.
FAU - Renault, Tristan
AU  - Renault T
AD  - Ifremer, Departement Ressources Biologiques et Environnement, La Tremblade, 
      France.
FAU - Morga, Benjamin
AU  - Morga B
AD  - Ifremer, ASIM, Adaptation Sante des invertebres, La Tremblade, France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220404
PL  - Switzerland
TA  - Front Cell Infect Microbiol
JT  - Frontiers in cellular and infection microbiology
JID - 101585359
RN  - 0 (DNA, Viral)
RN  - Ostreid herpesvirus 1
SB  - IM
MH  - Animals
MH  - Autophagy
MH  - *Crassostrea/genetics/metabolism
MH  - DNA Viruses
MH  - DNA, Viral/analysis
MH  - *Herpesviridae
MH  - Mammals/genetics
MH  - *Virus Diseases
PMC - PMC9014014
OTO - NOTNLM
OT  - Pacific oyster (Crassostrea gigas)
OT  - autophagy
OT  - herpesvirus
OT  - innate immunity
OT  - invertebrate
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/04/22 06:00
MHDA- 2022/04/23 06:00
PMCR- 2022/01/01
CRDT- 2022/04/21 05:45
PHST- 2022/01/19 00:00 [received]
PHST- 2022/03/01 00:00 [accepted]
PHST- 2022/04/21 05:45 [entrez]
PHST- 2022/04/22 06:00 [pubmed]
PHST- 2022/04/23 06:00 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fcimb.2022.858311 [doi]
PST - epublish
SO  - Front Cell Infect Microbiol. 2022 Apr 4;12:858311. doi: 
      10.3389/fcimb.2022.858311. eCollection 2022.