PMID- 35445947
OWN - NLM
STAT- MEDLINE
DCOM- 20220425
LR  - 20220425
IS  - 1573-7438 (Electronic)
IS  - 0049-4747 (Linking)
VI  - 54
IP  - 3
DP  - 2022 Apr 20
TI  - Monensin supplementation downregulated the expression signature of genes involved 
      in cholesterol synthesis in the ruminal epithelium and adipose tissue of lambs.
PG  - 167
LID - 10.1007/s11250-022-03168-w [doi]
AB  - To understand the metabolic mechanisms regulating lipid metabolism by monensin, 
      Afshari male lambs (n = 16) with 41.0 +/- 2.4 kg body weight (BW, mean +/- SD) at 
      approximately 180 days of age were randomly assigned equally to two dietary 
      treatments. After a 21-day pre-adaptation period, all animals in two groups 
      continued to receive the basal diet, but one group received no monensin 
      supplementation (control) while the other group received 30 mg/day of monensin 
      per animal. Individual BW was recorded weekly to determine the average daily body 
      weight gain (ADG). At the end of the 56-day experimental period, lambs were 
      weighed and slaughtered. Monensin supplementation did not affect BW, ADG, and 
      rumen fermentation characteristics. However, monensin significantly downregulated 
      the sterol regulatory element-binding protein (SREBP)-2 gene expression in all 
      sample tissues (p < 0.05). Also, monensin downregulated expressions of SREBP-1c 
      and peroxisome proliferator-activated receptor (PPAR)-gamma in back fat tissues. 
      Monensin increased the expression of 3-hydroxy-3-methylglutaryl-CoA synthase 
      (HMGCS)-2, but it decreased the mRNA abundance of HMGCS-1 in the rumen epithelial 
      tissues (p < 0.05). Our data suggest that monensin downregulates cholesterol 
      synthesis via inhibition of HMGCS-1 and impairment of the SREBP pathway, probably 
      due to a crosstalk among different tissues to control energy metabolism.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer Nature B.V.
FAU - Mirzaei-Alamouti, H
AU  - Mirzaei-Alamouti H
AD  - Department of Animal Science, University of Zanjan, 45371-38791, Zanjan, Iran. 
      alamoutih@znu.ac.ir.
FAU - Moradi, S
AU  - Moradi S
AD  - Department of Animal Science, University of Zanjan, 45371-38791, Zanjan, Iran.
FAU - Patra, A K
AU  - Patra AK
AD  - Department of Animal Nutrition, Bengal University of Animal and Fishery Sciences, 
      West, Kolkata, 700037, India.
FAU - Mansouryar, M
AU  - Mansouryar M
AD  - Zist Dam Group, University Incubator Center, University of Zanjan, 45371-38791, 
      Zanjan, Iran. mansooryar@znu.ac.ir.
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial, Veterinary
DEP - 20220420
PL  - United States
TA  - Trop Anim Health Prod
JT  - Tropical animal health and production
JID - 1277355
RN  - 0 (Sterol Regulatory Element Binding Protein 1)
RN  - 906O0YJ6ZP (Monensin)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Adipose Tissue
MH  - Animal Feed/analysis
MH  - Animals
MH  - Body Weight
MH  - Cholesterol/metabolism
MH  - Diet/veterinary
MH  - Dietary Supplements
MH  - Epithelium/metabolism
MH  - Male
MH  - *Monensin/metabolism/pharmacology
MH  - *Rumen/metabolism
MH  - Sheep
MH  - Sheep, Domestic
MH  - Sterol Regulatory Element Binding Protein 1/metabolism
OTO - NOTNLM
OT  - 3-Hydroxy-3-methylglutaryl-CoA synthase
OT  - Growth performance
OT  - Monensin
OT  - Ruminal fermentation
OT  - Sheep
OT  - Sterol regulatory element-binding protein
EDAT- 2022/04/22 06:00
MHDA- 2022/04/26 06:00
CRDT- 2022/04/21 12:07
PHST- 2021/10/14 00:00 [received]
PHST- 2022/04/07 00:00 [accepted]
PHST- 2022/04/21 12:07 [entrez]
PHST- 2022/04/22 06:00 [pubmed]
PHST- 2022/04/26 06:00 [medline]
AID - 10.1007/s11250-022-03168-w [pii]
AID - 10.1007/s11250-022-03168-w [doi]
PST - epublish
SO  - Trop Anim Health Prod. 2022 Apr 20;54(3):167. doi: 10.1007/s11250-022-03168-w.