PMID- 35447396
OWN - NLM
STAT- MEDLINE
DCOM- 20220524
LR  - 20220524
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 210
DP  - 2022 Aug 15
TI  - Plasmonic Au nanocube enhanced SERS biosensor based on heated electrode and 
      strand displacement amplification for highly sensitive detection of Dam 
      methyltransferase activity.
PG  - 114283
LID - S0956-5663(22)00323-2 [pii]
LID - 10.1016/j.bios.2022.114283 [doi]
AB  - In this work, a novel "turn-on" mode Au nanocubes (AuNCs) enhanced 
      surface-enhanced Raman scattering (SERS) biosensing platform coupled with heated 
      Au electrode (HAuE) and strand displacement amplification (SDA) strategy was 
      proposed for highly sensitive detection of DNA adenine methylation (Dam) 
      Methyltransferase (MTase) activity. The Dam MTase and DpnI enzyme activities were 
      significantly increased by elevating the HAuE surface temperature, resulting in 
      the rapid production of template DNA for later SDA. During the SDA process, the 
      released single-stranded DNA (ssDNA) could be amplified exponentially, and its 
      concentration was positively related to the Dam MTase activity. The plasmonic 
      AuNCs in SERS tags could provide significant SERS enhancement due to their 
      "lightning rod" effect resulting from the sharp feature of the edges and corners 
      of AuNCs. Because of these factors, the proposed biosensors exhibited high 
      sensitivity in detecting the Dam MTase activity. The limit of detection was 
      estimated to be 8.65 x 10(-5) U mL(-1), which was lower than that in most of the 
      sensors for detection of Dam MTase activity in the literature. This SERS 
      biosensor could also be used to screen inhibitors of Dam MTase and had the 
      potential for detecting Dam MTase activity in real biological samples.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Hu, Hao-Cheng
AU  - Hu HC
AD  - Ministry of Education Key Laboratory for Analytical Science of Food Safety and 
      Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology 
      for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350108, 
      China.
FAU - Wu, Shao-Hua
AU  - Wu SH
AD  - Ministry of Education Key Laboratory for Analytical Science of Food Safety and 
      Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology 
      for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350108, 
      China. Electronic address: wsh@fzu.edu.cn.
FAU - Jin, Lei-Xin
AU  - Jin LX
AD  - Ministry of Education Key Laboratory for Analytical Science of Food Safety and 
      Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology 
      for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350108, 
      China.
FAU - Sun, Jian-Jun
AU  - Sun JJ
AD  - Ministry of Education Key Laboratory for Analytical Science of Food Safety and 
      Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology 
      for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian, 350108, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20220412
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (DNA, Single-Stranded)
RN  - EC 2.1.1.72 (Dam methyltransferase)
RN  - EC 2.1.1.72 (Site-Specific DNA-Methyltransferase (Adenine-Specific))
SB  - IM
MH  - *Biosensing Techniques/methods
MH  - DNA Methylation
MH  - DNA, Single-Stranded
MH  - Electrodes
MH  - Site-Specific DNA-Methyltransferase (Adenine-Specific)
OTO - NOTNLM
OT  - Au nanocube
OT  - Dam methyltransferase
OT  - Heated electrode
OT  - Strand displacement amplification (SDA)
OT  - Surface enhanced Raman spectroscopy (SERS)
EDAT- 2022/04/22 06:00
MHDA- 2022/05/25 06:00
CRDT- 2022/04/21 20:12
PHST- 2022/02/14 00:00 [received]
PHST- 2022/04/03 00:00 [revised]
PHST- 2022/04/10 00:00 [accepted]
PHST- 2022/04/22 06:00 [pubmed]
PHST- 2022/05/25 06:00 [medline]
PHST- 2022/04/21 20:12 [entrez]
AID - S0956-5663(22)00323-2 [pii]
AID - 10.1016/j.bios.2022.114283 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2022 Aug 15;210:114283. doi: 10.1016/j.bios.2022.114283. 
      Epub 2022 Apr 12.