PMID- 35448663 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220716 IS - 2306-7381 (Electronic) IS - 2306-7381 (Linking) VI - 9 IP - 4 DP - 2022 Mar 29 TI - Development of a Chimeric Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)-2 Vaccine Candidate Expressing Hypo-Glycosylated Glycoprotein-5 Ectodomain of Korean Lineage-1 Strain. LID - 10.3390/vetsci9040165 [doi] LID - 165 AB - Vaccination is a practical method to provide protection against porcine reproductive and respiratory syndrome virus (PRRSV), but current PRRSV vaccines show limited efficacy against divergent field strains. Lineage 1 PRRSV includes virulent strains such as NADC30 and MN184 and now has become one of the most prevalent viruses in Korea. Accordingly, there is an urgent need to develop a new vaccine for Korean lineage-1 strains. In this study, a vaccine candidate against Korean lineage-1 PRRSV, vCSL1-GP5-N33D, was developed by reverse genetics technology. vCSL1-GP5-N33D was designed as a hypo-glycosylated chimeric virus containing the glycoprotein 5 ectodomain region of the Korean lineage-1 wild-type strain. An inactivated vaccine of vCSL1-GP5-N33D was applied to a PRRS-endemic farm and elicited high serum virus neutralization (SVN) antibody titers. The vaccinated group induced SVN antibody titers of 4.40 (log(2)) +/- 2.46, which were approximately 2-fold higher than those of the negative control at 8-weeks post-vaccination. Moreover, 60% of pigs in the vaccinated group displayed SVN antibody titers of >/=5 (log(2)), while none of the pigs in the negative control exhibited SVN antibody titers of >/=5 (log(2)). The overall results of the animal experiment suggest that the vCSL1-GP5-N33D inactivated vaccine is a promising vaccine candidate. FAU - Choi, Hwi-Yeon AU - Choi HY AUID- ORCID: 0000-0001-7773-0982 AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea. FAU - Kim, Min-Sik AU - Kim MS AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea. FAU - Kang, Yeong-Lim AU - Kang YL AUID- ORCID: 0000-0001-6940-4271 AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea. FAU - Choi, Jong-Chul AU - Choi JC AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea. FAU - Choi, In-Yeong AU - Choi IY AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea. FAU - Jung, Sung-Won AU - Jung SW AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea. FAU - Jeong, Ji-Yun AU - Jeong JY AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea. AD - Careside Co. Ltd., Woolim Lions Valley A-B210, #146-8, Sangdaewon-dong, Jungwon-gu, Seongnam 13209, Gyeonggi-do, Korea. FAU - Kim, Min-Chul AU - Kim MC AD - Careside Co. Ltd., Woolim Lions Valley A-B210, #146-8, Sangdaewon-dong, Jungwon-gu, Seongnam 13209, Gyeonggi-do, Korea. FAU - Hwang, Seong-Soo AU - Hwang SS AD - Samhwa Breedings Agri. Inc., 435, Sinjin-ri, Gwangcheon-eup, Hongseong-gun 35090, Chungcheongnam-do, Korea. FAU - Lee, Sang-Won AU - Lee SW AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea. AD - KU Research Center for Zoonosis, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea. FAU - Park, Seung-Yong AU - Park SY AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea. AD - KU Research Center for Zoonosis, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea. FAU - Song, Chang-Seon AU - Song CS AUID- ORCID: 0000-0002-4158-6402 AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea. AD - KU Research Center for Zoonosis, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea. FAU - Choi, In-Soo AU - Choi IS AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea. AD - KU Research Center for Zoonosis, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea. FAU - Lee, Joong-Bok AU - Lee JB AUID- ORCID: 0000-0003-1074-8162 AD - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk University, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea. AD - KU Research Center for Zoonosis, 120 Neungdong-ro, Gwangjin-gu, Seoul 05029, Korea. LA - eng GR - Not applicable/Careside/ GR - Not applicalbe/Konkuk University/ GR - 2019R1A6A3A13096813/National Research Foundation of Korea/ GR - 320005-04/Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries/ PT - Journal Article DEP - 20220329 PL - Switzerland TA - Vet Sci JT - Veterinary sciences JID - 101680127 PMC - PMC9028511 OTO - NOTNLM OT - GP5 OT - Korea OT - PRRSV OT - chimera OT - neutralizing antibodies OT - vaccine COIS- J.-Y.J. and M.-C.K. are employees of Careside (Seongnam, Korea). The rest of the authors declare that they have no relevant conflict of interest. EDAT- 2022/04/22 06:00 MHDA- 2022/04/22 06:01 PMCR- 2022/03/29 CRDT- 2022/04/21 20:20 PHST- 2022/02/23 00:00 [received] PHST- 2022/03/20 00:00 [revised] PHST- 2022/03/26 00:00 [accepted] PHST- 2022/04/21 20:20 [entrez] PHST- 2022/04/22 06:00 [pubmed] PHST- 2022/04/22 06:01 [medline] PHST- 2022/03/29 00:00 [pmc-release] AID - vetsci9040165 [pii] AID - vetsci-09-00165 [pii] AID - 10.3390/vetsci9040165 [doi] PST - epublish SO - Vet Sci. 2022 Mar 29;9(4):165. doi: 10.3390/vetsci9040165.