PMID- 35448958
OWN - NLM
STAT- MEDLINE
DCOM- 20220425
LR  - 20220716
IS  - 1471-230X (Electronic)
IS  - 1471-230X (Linking)
VI  - 22
IP  - 1
DP  - 2022 Apr 21
TI  - Nuclear respiratory factor 1 promotes the growth of liver hepatocellular 
      carcinoma cells via E2F1 transcriptional activation.
PG  - 198
LID - 10.1186/s12876-022-02260-7 [doi]
LID - 198
AB  - BACKGROUND: Recent studies have shown that functional mitochondria are essential 
      for cancer cells. Nuclear respiratory factor 1 (NRF1) is a transcription factor 
      that activates mitochondrial biogenesis and the expression of the respiratory 
      chain, but little is known about its role and underlying mechanism in liver 
      hepatocellular carcinoma (LIHC). METHODS: NRF1 expression was analyzed via public 
      databases and 24 paired LIHC samples. Clinical-pathological information and 
      follow-up data were collected from 165 patients with LIHC or online datasets. 
      Furthermore, cellular proliferation and the cell cycle were analyzed by MTT, 
      Clone-forming assay and flow cytometric analyses. NRF1 target genes were analyzed 
      by Chromatin immunoprecipitation sequencing (ChIP-Seq). PCR and WB analysis was 
      performed to detect the expression of related genes. ChIP and luciferase activity 
      assays were used to identify NRF1 binding sites. RESULTS: Our results showed that 
      NRF1 expression was upregulated in LIHC compared to normal tissues. NRF1 
      expression was associated with tumour size and poor prognosis in patients. 
      Knockdown of NRF1 repressed cell proliferation and overexpression of NRF1 
      accelerated the G(1)/S phase transition. Additionally, data from ChIP-seq pointed 
      out that some NRF1 target genes are involved in the cell cycle. Our findings 
      indicated that NRF1 directly binds to the E2F1 promoter as a transcription factor 
      and regulates its gene expression. CONCLUSION: Therefore, this study revealed 
      that NRF1 promotes cancer cell growth via the indirect transcriptional activation 
      of E2F1 and is a potential biomarker in LIHC.
CI  - (c) 2022. The Author(s).
FAU - Wang, Dan
AU  - Wang D
AD  - Institute of Special Environmental Medicine, Nantong University, 9 Se Yuan Road, 
      Nantong, 226019, Jiangsu, China.
FAU - Wan, Baolan
AU  - Wan B
AD  - Institute of Special Environmental Medicine, Nantong University, 9 Se Yuan Road, 
      Nantong, 226019, Jiangsu, China.
FAU - Zhang, Xiaojing
AU  - Zhang X
AD  - Department of Clinical Biobank, Affiliated Hospital of Nantong University, 
      Nantong, 226001, Jiangsu, China.
FAU - Sun, Pingping
AU  - Sun P
AD  - Department of Clinical Biobank, Affiliated Hospital of Nantong University, 
      Nantong, 226001, Jiangsu, China.
FAU - Lu, Shu
AU  - Lu S
AD  - Department of Intensive Care Unit, Affiliated Hospital of Nantong University, 
      Nantong, 226001, Jiangsu, China.
FAU - Liu, Chenxu
AU  - Liu C
AD  - Department of Biochemistry and Molecular Biology, Medical School, Nantong 
      University, Nantong, 226001, Jiangsu, China.
FAU - Zhu, Li
AU  - Zhu L
AD  - Institute of Special Environmental Medicine, Nantong University, 9 Se Yuan Road, 
      Nantong, 226019, Jiangsu, China. zhulizhou@ntu.edu.cn.
LA  - eng
GR  - 81702874/National Natural Science Foundation of China/
GR  - 31471141/National Natural Science Foundation of China/
GR  - 2020/Special Foundation for Excellent Young Teachers and Principals Program of 
      Jiangsu Province/
GR  - MB2020081/Nantong Commission of Health/
GR  - 202110304032Z/National College Students' innovation and entrepreneurship training 
      program/
PT  - Journal Article
DEP - 20220421
PL  - England
TA  - BMC Gastroenterol
JT  - BMC gastroenterology
JID - 100968547
RN  - 0 (E2F1 Transcription Factor)
RN  - 0 (E2F1 protein, human)
RN  - 0 (Nuclear Respiratory Factor 1)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - *Carcinoma, Hepatocellular/genetics/metabolism
MH  - E2F1 Transcription Factor/genetics/metabolism
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - *Liver Neoplasms/genetics/metabolism
MH  - Nuclear Respiratory Factor 1/genetics/metabolism
MH  - Transcription Factors/genetics
MH  - Transcriptional Activation
PMC - PMC9027447
OTO - NOTNLM
OT  - Cell proliferation
OT  - Gene expression regulation
OT  - Hepatocellular carcinoma
OT  - Prognosis
COIS- The authors declare that they have no competing interests.
EDAT- 2022/04/23 06:00
MHDA- 2022/04/26 06:00
PMCR- 2022/04/21
CRDT- 2022/04/22 05:04
PHST- 2021/08/30 00:00 [received]
PHST- 2022/04/04 00:00 [accepted]
PHST- 2022/04/22 05:04 [entrez]
PHST- 2022/04/23 06:00 [pubmed]
PHST- 2022/04/26 06:00 [medline]
PHST- 2022/04/21 00:00 [pmc-release]
AID - 10.1186/s12876-022-02260-7 [pii]
AID - 2260 [pii]
AID - 10.1186/s12876-022-02260-7 [doi]
PST - epublish
SO  - BMC Gastroenterol. 2022 Apr 21;22(1):198. doi: 10.1186/s12876-022-02260-7.