PMID- 35450807 OWN - NLM STAT- MEDLINE DCOM- 20221025 LR - 20221025 IS - 1473-0502 (Print) IS - 1473-0502 (Linking) VI - 61 IP - 5 DP - 2022 Oct TI - The determinants of donor safety and product quality in optimization of apheresis granulocyte harvest: An experience from a tertiary care oncology centre in India. PG - 103445 LID - S1473-0502(22)00118-5 [pii] LID - 10.1016/j.transci.2022.103445 [doi] AB - Allogeneic granulocyte transfusions play a substantial role in treatment of lifethreatening neutropenia-associated infections in patients undergoing intensive chemotherapy and hematopoietic stem cell transplant. Granulocyte harvest by apheresis is considered a safe and effective method to obtain adequate therapeutic granulocyte dosage for clinical effectiveness. This study described the experiences associated with apheresis granulocyte harvest procedures in our tertiary care haemato-oncology centre. We have analysed the incidence of adverse events (AEs) with associated potential risk factors contributing to donor safety and improvement in product quality. Retrospective data of 131 healthy allogeneic donors who underwent granulocyte harvest from May 2016 to July 2020 were analyzed. AEs were observed in overall 29 procedures (22.13%), including 14.50% citrate reactions, 7.6% venous access-related reactions, and 1.52% vasovagal reactions. Older age (p = 0.012) and higher body mass index (p = 0.015) in donors were significant variables found associated with a higher incidence of AEs. There was no significant impact of AEs on granulocyte product yield (p = 0.41) with a median collection yield of 1.73 x 10 10 cells/ unit. In multivariate analysis, post-mobilization parameters like total leukocyte counts (p = 0.036), absolute neutrophil counts (p = 0.042), and platelet counts (p = 0.006) showed a positive correlation with higher product yield. All the donors successfully donated and tolerated granulocyte colony stimulating factor plus dexamethasone mobilization and granulocyte apheresis harvest without any serious AEs. Our study shows that optimal technical and procedural modifications during apheresis granulocyte harvest procedures can overcome the associated potential risks by providing donor safety and improving product quality. CI - Copyright (c) 2022 Elsevier Ltd. All rights reserved. FAU - Ojha, Shashank AU - Ojha S AD - Department of Transfusion Medicine, Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Homi Bhabha National Institute, Navi Mumbai 410210, India. Electronic address: sojha@actrec.gov.in. FAU - Poojary, Minal AU - Poojary M AD - Department of Transfusion Medicine, Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Homi Bhabha National Institute, Navi Mumbai 410210, India. Electronic address: mpoojary@actrec.gov.in. FAU - Mokalikar, Umakant AU - Mokalikar U AD - Department of Transfusion Medicine, Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Homi Bhabha National Institute, Navi Mumbai 410210, India. Electronic address: mokalikarumakant1234@gmail.com. FAU - Sumathi, S H AU - Sumathi SH AD - Department of Transfusion Medicine, Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Homi Bhabha National Institute, Navi Mumbai 410210, India. Electronic address: sumathish@actrec.gov.in. FAU - Kumar, Arun AU - Kumar A AD - Department of Transfusion Medicine, Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Homi Bhabha National Institute, Navi Mumbai 410210, India. Electronic address: arun.actrec@yahoo.co.in. FAU - Gupta, Abhaykumar Malind AU - Gupta AM AD - Department of Transfusion Medicine, Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Homi Bhabha National Institute, Navi Mumbai 410210, India. Electronic address: dr.abhaymgupta@gmail.com. FAU - Saha, Suryatapa AU - Saha S AD - Department of Transfusion Medicine, Advanced Centre for Treatment, Research and Education in Cancer, Tata Memorial Centre, Homi Bhabha National Institute, Navi Mumbai 410210, India. Electronic address: ssaha@actrec.gov.in. LA - eng PT - Journal Article DEP - 20220407 PL - England TA - Transfus Apher Sci JT - Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis JID - 101095653 RN - 143011-72-7 (Granulocyte Colony-Stimulating Factor) RN - 7S5I7G3JQL (Dexamethasone) RN - 0 (Citrates) MH - Humans MH - Retrospective Studies MH - Tertiary Healthcare MH - *Blood Component Removal MH - Granulocyte Colony-Stimulating Factor MH - Granulocytes MH - India MH - Dexamethasone MH - Citrates OTO - NOTNLM OT - Adverse events OT - Donor safety OT - Granulocyte apheresis OT - Granulocyte donor OT - Granulocyte product COIS- Conflict of interest The authors declare no potential conflict of interest. EDAT- 2022/04/23 06:00 MHDA- 2022/10/26 06:00 CRDT- 2022/04/22 07:03 PHST- 2022/02/10 00:00 [received] PHST- 2022/04/02 00:00 [revised] PHST- 2022/04/05 00:00 [accepted] PHST- 2022/04/23 06:00 [pubmed] PHST- 2022/10/26 06:00 [medline] PHST- 2022/04/22 07:03 [entrez] AID - S1473-0502(22)00118-5 [pii] AID - 10.1016/j.transci.2022.103445 [doi] PST - ppublish SO - Transfus Apher Sci. 2022 Oct;61(5):103445. doi: 10.1016/j.transci.2022.103445. Epub 2022 Apr 7.