PMID- 35451594
OWN - NLM
STAT- MEDLINE
DCOM- 20220621
LR  - 20220621
IS  - 1432-2242 (Electronic)
IS  - 0040-5752 (Linking)
VI  - 135
IP  - 6
DP  - 2022 Jun
TI  - Cytogenetic identification and molecular marker development of a novel 
      wheat-Thinopyrum ponticum translocation line with powdery mildew resistance.
PG  - 2041-2057
LID - 10.1007/s00122-022-04092-1 [doi]
AB  - A new wheat-Thinopyrum ponticum translocation line with excellent powdery mildew 
      resistance was produced, and alien-specific PCR markers and FISH probes were 
      developed by SLAF-seq. Powdery mildew is one of the most threatening diseases in 
      wheat production. Thinopyrum ponticum (Podp.) Barkworth and D. R. Dewey, as a 
      wild relative, has been used for wheat genetic improvement for the better part of 
      a century. In view of the good powdery mildew resistance of Th. ponticum, we have 
      been working to transfer the resistance genes from Th. ponticum to wheat by 
      creating translocation lines. In this study, a new wheat-Th. ponticum 
      translocation line with excellent resistance and agronomic performance was 
      developed and through seedling disease evaluation, gene postulation and 
      diagnostic marker analysis proved to carry a novel Pm gene derived from Th. 
      ponticum. Cytogenetic analysis revealed that a small alien segment was 
      translocated to the terminal of chromosome 1D to form new translocation 
      TTh-1DS.1DL chromosome. The translocation breakpoint was determined to lie in 
      21.5 Mb region of chromosome 1D by using Wheat660K SNP array analysis. Based on 
      specific-locus amplified fragment sequencing (SLAF-seq) technology, eight 
      molecular markers and one repetitive sequence probe were developed, which were 
      specific for Th. ponticum. Fortunately, the probe could be used in distinguishing 
      six alien chromosome pairs in partial amphiploid Xiaoyan 7430 by fluorescence in 
      situ hybridization (FISH). Furthermore, a Thinopyrum-specific oligonucleotide 
      probe was designed depending on the sequence information of the FISH probe. The 
      novel translocation line could be used in wheat disease resistance breeding, and 
      these specific markers and probes will enable wheat breeders to rapidly trace the 
      alien genome with the novel Pm gene(s).
CI  - (c) 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, 
      part of Springer Nature.
FAU - Yang, Guotang
AU  - Yang G
AD  - State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of 
      Genetics and Developmental Biology, The Innovative Academy of Seed Design, 
      Chinese Academy of Sciences, Beijing, 100101, China.
AD  - University of Chinese Academy of Sciences, Beijing, 100049, China.
FAU - Tong, Chunyan
AU  - Tong C
AD  - State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of 
      Genetics and Developmental Biology, The Innovative Academy of Seed Design, 
      Chinese Academy of Sciences, Beijing, 100101, China.
AD  - College of Life Science and Technology, Inner Mongolia Normal University, Hohhot, 
      010022, China.
FAU - Li, Hongwei
AU  - Li H
AD  - State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of 
      Genetics and Developmental Biology, The Innovative Academy of Seed Design, 
      Chinese Academy of Sciences, Beijing, 100101, China.
FAU - Li, Bin
AU  - Li B
AD  - State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of 
      Genetics and Developmental Biology, The Innovative Academy of Seed Design, 
      Chinese Academy of Sciences, Beijing, 100101, China.
FAU - Li, Zhensheng
AU  - Li Z
AD  - State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of 
      Genetics and Developmental Biology, The Innovative Academy of Seed Design, 
      Chinese Academy of Sciences, Beijing, 100101, China.
FAU - Zheng, Qi
AU  - Zheng Q
AUID- ORCID: 0000-0001-9600-6607
AD  - State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of 
      Genetics and Developmental Biology, The Innovative Academy of Seed Design, 
      Chinese Academy of Sciences, Beijing, 100101, China. qzheng@genetics.ac.cn.
LA  - eng
GR  - 31971875/National Natural Science Foundation of China/
GR  - 2016YFD0102000/National Key Research and Development Program of China/
PT  - Journal Article
DEP - 20220422
PL  - Germany
TA  - Theor Appl Genet
JT  - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
JID - 0145600
SB  - IM
MH  - *Chromosomes, Plant/genetics
MH  - Cytogenetic Analysis
MH  - Disease Resistance/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Plant Breeding
MH  - Plant Diseases/genetics
MH  - Poaceae/genetics
MH  - Translocation, Genetic
MH  - *Triticum/genetics
EDAT- 2022/04/23 06:00
MHDA- 2022/06/22 06:00
CRDT- 2022/04/22 12:00
PHST- 2021/12/22 00:00 [received]
PHST- 2022/03/26 00:00 [accepted]
PHST- 2022/04/23 06:00 [pubmed]
PHST- 2022/06/22 06:00 [medline]
PHST- 2022/04/22 12:00 [entrez]
AID - 10.1007/s00122-022-04092-1 [pii]
AID - 10.1007/s00122-022-04092-1 [doi]
PST - ppublish
SO  - Theor Appl Genet. 2022 Jun;135(6):2041-2057. doi: 10.1007/s00122-022-04092-1. 
      Epub 2022 Apr 22.