PMID- 35452062 OWN - NLM STAT- MEDLINE DCOM- 20220511 LR - 20220531 IS - 2042-650X (Electronic) IS - 2042-6496 (Linking) VI - 13 IP - 9 DP - 2022 May 10 TI - Camellia (Camellia oleifera bel.) seed oil reprograms gut microbiota and alleviates lipid accumulation in high fat-fed mice through the mTOR pathway. PG - 4977-4992 LID - 10.1039/d1fo04075h [doi] AB - Camellia (Camellia oleifera bel.) seed oil (CO) is extensively used as an edible oil in China and Asian countries owing to its high nutritional and medicinal values. It has been shown that a high-fat diet enhances lipid accumulation and induces intestinal microbiota imbalance in mice. However, it is still to be learned whether CO prevents dyslipidemia through gut microbiota. Here, using 16S rRNA gene sequencing analysis of the gut microbiota, we found that oral CO relieved lipid accumulation and reversed gut microbiota dysbiosis. Compared to mice (C57BL/6J male mice) fed a high-fat diet, treatment with CO regulated the composition and functional profiling communities related to the lipid metabolism of gut microbiota. The abundances of Dubosiella, Lactobacillus, and Alistipes were markedly increased in CO supplementation mice. In addition, the colon levels of isobutyric acid, pentanoic acid, and isovaleric acid were similar between the control and CO supplementation mice. Besides, the results indicated that CO supplementation in mice alleviated lipid droplet accumulation in the hepatocytes and subcutaneous adipose tissue, although the liver index did not show a difference. Notably, CO supplementation for 6 weeks significantly reduced the levels of LDL, TC, and TG, while enhancing the level of HDL in serum and liver. Meanwhile, we also identified that CO supplementation suppressed the mammalian target of rapamycin (mTOR) signaling pathway in high fat-fed (HF-fed) mice. Taken together, our results suggest that CO improved dyslipidemia and alleviated lipid accumulation in HF-fed mice, the molecular mechanisms possibly associated with the reorganization of gut microbiota, in particular, Alistipes and Dubosiella, mediated the inhibition of the mTOR pathway. FAU - Gao, Jing AU - Gao J AUID- ORCID: 0000-0002-4083-4232 AD - Research Institute of Oil Tea Camellia, Hunan Academy of Forestry, Shao shan South Road, No. 658, Changsha 410004, China. chenyongzhong06@163.com. AD - National Engineering Research Center for Oil Tea Camellia, Changsha 410004, China. AD - Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Chinese Academy of Sciences, Changsha, Hunan, China. yinyulong@isa.ac.cn. FAU - Ma, Li AU - Ma L AD - Research Institute of Oil Tea Camellia, Hunan Academy of Forestry, Shao shan South Road, No. 658, Changsha 410004, China. chenyongzhong06@163.com. AD - National Engineering Research Center for Oil Tea Camellia, Changsha 410004, China. FAU - Yin, Jie AU - Yin J AUID- ORCID: 0000-0003-1852-1042 AD - College of Animal Science and Technology, Hunan Co-Innovation Center of Animal Production Safety, Hunan Agricultural University, Changsha, China. FAU - Liu, Gang AU - Liu G AD - College of Animal Science and Technology, Hunan Co-Innovation Center of Animal Production Safety, Hunan Agricultural University, Changsha, China. FAU - Ma, Jie AU - Ma J AD - College of Animal Science and Technology, Hunan Co-Innovation Center of Animal Production Safety, Hunan Agricultural University, Changsha, China. FAU - Xia, SiTing AU - Xia S AD - College of Animal Science and Technology, Hunan Co-Innovation Center of Animal Production Safety, Hunan Agricultural University, Changsha, China. FAU - Gong, SaiMing AU - Gong S AD - College of Animal Science and Technology, Hunan Co-Innovation Center of Animal Production Safety, Hunan Agricultural University, Changsha, China. FAU - Han, Qi AU - Han Q AD - College of Animal Science and Technology, Hunan Co-Innovation Center of Animal Production Safety, Hunan Agricultural University, Changsha, China. FAU - Li, TieJun AU - Li T AD - Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Chinese Academy of Sciences, Changsha, Hunan, China. yinyulong@isa.ac.cn. FAU - Chen, YongZhong AU - Chen Y AD - Research Institute of Oil Tea Camellia, Hunan Academy of Forestry, Shao shan South Road, No. 658, Changsha 410004, China. chenyongzhong06@163.com. AD - National Engineering Research Center for Oil Tea Camellia, Changsha 410004, China. FAU - Yin, YuLong AU - Yin Y AD - Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process, Chinese Academy of Sciences, Changsha, Hunan, China. yinyulong@isa.ac.cn. AD - College of Animal Science and Technology, Hunan Co-Innovation Center of Animal Production Safety, Hunan Agricultural University, Changsha, China. LA - eng PT - Journal Article DEP - 20220510 PL - England TA - Food Funct JT - Food & function JID - 101549033 RN - 0 (Lipids) RN - 0 (Plant Oils) RN - 0 (RNA, Ribosomal, 16S) RN - EC 2.7.11.1 (TOR Serine-Threonine Kinases) SB - IM MH - Animals MH - *Camellia MH - Diet, High-Fat/adverse effects MH - *Dyslipidemias MH - *Gastrointestinal Microbiome MH - Lipid Metabolism MH - Lipids MH - Male MH - Mice MH - Mice, Inbred C57BL MH - Plant Oils/metabolism MH - RNA, Ribosomal, 16S/metabolism MH - TOR Serine-Threonine Kinases/genetics/metabolism EDAT- 2022/04/23 06:00 MHDA- 2022/05/12 06:00 CRDT- 2022/04/22 12:13 PHST- 2022/04/23 06:00 [pubmed] PHST- 2022/05/12 06:00 [medline] PHST- 2022/04/22 12:13 [entrez] AID - 10.1039/d1fo04075h [doi] PST - epublish SO - Food Funct. 2022 May 10;13(9):4977-4992. doi: 10.1039/d1fo04075h.