PMID- 35452301
OWN - NLM
STAT- MEDLINE
DCOM- 20220426
LR  - 20220531
IS  - 2578-5478 (Electronic)
IS  - 2578-5478 (Linking)
VI  - 40
IP  - 4
DP  - 2022 Apr
TI  - Web Crawling and mRNA Sequencing Analyze Mechanisms of Photobiomodulation.
PG  - 252-260
LID - 10.1089/photob.2021.0142 [doi]
AB  - Background: Photobiomodulation (PBM) is praised as a promising physical therapy, 
      which has many advantages, such as being noninvasive and painless. However, the 
      mechanisms are not fully elucidated. Methods: Using web crawling, mRNA sequence, 
      and bioinformatics analysis, we selected genes, functional annotation, and 
      mechanisms. The expressions of inflammatory cytokines were measured using 
      quantitative real-time PCR (RT-qPCR). Results: A total of 146 human genes and 57 
      pathways were identified about PBM. The 630 nm light-emitting diode 
      (LED)-stimulated-MH7A cells were sequenced to further analyze the mechanism of 
      PBM. Two thousand nine hundred fifty differentially expressed genes were 
      identified, and the gene ontology term enrichment analysis and Kyoto encyclopedia 
      of genes and genomes pathway analysis were performed to better understand 
      functions and pathways. The 12 pathways were matched with the KEGG results of PBM 
      and MH7A cells. A protein-protein interaction network was performed among genes 
      in 12 pathways, and 10 outstanding proteins were identified. Importantly, the 9 
      genes were predicted with potential research value. And we also demonstrated that 
      expression of inflammatory factors [interleukin (IL)-6, IL-1beta, IL-8, and matrix 
      metalloproteinase-3 (MMP-3)] was reduced; meanwhile, the expression of 
      anti-inflammatory factor IL-10 was promoted after 630 nm LED. Conclusions: Using 
      web crawling, bioinformatics analysis, and mRNA sequence, we obtained 9 key genes 
      and 12 important pathways about PBM. Importantly, we demonstrated the 
      anti-inflammatory effect of 630 nm LED red light by RT-qPCR.
FAU - Wu, Hao
AU  - Wu H
AD  - The Heilongjiang Key Laboratory of Immunity and Infection Harbin Medical 
      University, Department of Microbiology, Wu Lien-Teh Institute, Harbin, P.R. 
      China.
FAU - Liu, Qiannan
AU  - Liu Q
AD  - The Heilongjiang Key Laboratory of Immunity and Infection Harbin Medical 
      University, Department of Microbiology, Wu Lien-Teh Institute, Harbin, P.R. 
      China.
FAU - Meng, Caiyun
AU  - Meng C
AD  - The Heilongjiang Key Laboratory of Immunity and Infection Harbin Medical 
      University, Department of Microbiology, Wu Lien-Teh Institute, Harbin, P.R. 
      China.
FAU - Xia, Qing
AU  - Xia Q
AD  - The Heilongjiang Key Laboratory of Immunity and Infection Harbin Medical 
      University, Department of Microbiology, Wu Lien-Teh Institute, Harbin, P.R. 
      China.
FAU - Pan, Yue
AU  - Pan Y
AD  - The Heilongjiang Key Laboratory of Immunity and Infection Harbin Medical 
      University, Department of Microbiology, Wu Lien-Teh Institute, Harbin, P.R. 
      China.
FAU - Zhang, Hanxu
AU  - Zhang H
AD  - The Heilongjiang Key Laboratory of Immunity and Infection Harbin Medical 
      University, Department of Microbiology, Wu Lien-Teh Institute, Harbin, P.R. 
      China.
FAU - Zhang, Fengmin
AU  - Zhang F
AD  - The Heilongjiang Key Laboratory of Immunity and Infection Harbin Medical 
      University, Department of Microbiology, Wu Lien-Teh Institute, Harbin, P.R. 
      China.
FAU - Song, Wuqi
AU  - Song W
AUID- ORCID: 0000-0001-5900-5154
AD  - The Heilongjiang Key Laboratory of Immunity and Infection Harbin Medical 
      University, Department of Microbiology, Wu Lien-Teh Institute, Harbin, P.R. 
      China.
FAU - Liu, Hailiang
AU  - Liu H
AD  - The Heilongjiang Key Laboratory of Immunity and Infection Harbin Medical 
      University, Department of Microbiology, Wu Lien-Teh Institute, Harbin, P.R. 
      China.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Photobiomodul Photomed Laser Surg
JT  - Photobiomodulation, photomedicine, and laser surgery
JID - 101734781
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Cytokines)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Anti-Inflammatory Agents
MH  - Computational Biology
MH  - *Cytokines/genetics
MH  - Humans
MH  - Internet
MH  - *Low-Level Light Therapy
MH  - RNA, Messenger/genetics
OTO - NOTNLM
OT  - RT-qPCR
OT  - key genes
OT  - mRNA sequence
OT  - mechanisms
OT  - photobiomodulation
OT  - web crawling
EDAT- 2022/04/23 06:00
MHDA- 2022/04/27 06:00
CRDT- 2022/04/22 17:11
PHST- 2022/04/22 17:11 [entrez]
PHST- 2022/04/23 06:00 [pubmed]
PHST- 2022/04/27 06:00 [medline]
AID - 10.1089/photob.2021.0142 [doi]
PST - ppublish
SO  - Photobiomodul Photomed Laser Surg. 2022 Apr;40(4):252-260. doi: 
      10.1089/photob.2021.0142.