PMID- 35452863
OWN - NLM
STAT- MEDLINE
DCOM- 20220524
LR  - 20220524
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 269
DP  - 2022 Jun
TI  - miR-142-3p suppresses porcine reproductive and respiratory syndrome virus (PRRSV) 
      infection by directly targeting Rac1.
PG  - 109434
LID - S0378-1135(22)00104-3 [pii]
LID - 10.1016/j.vetmic.2022.109434 [doi]
AB  - Porcine reproductive and respiratory syndrome (PRRS) caused by PRRS virus (PRRSV) 
      has been recognized as one of the severest epidemics in pigs worldwide. microRNAs 
      (miRNAs) play important roles in a variety of biological processes, including 
      cell differentiation, proliferation and death, as well as viral infections and 
      antiviral immune responses. In this study, we found that miR-142-3p was expressed 
      lower in cells susceptible to PRRSV infection than in cells less or no permissive 
      to PRRSV infection. Subsequently, we showed that overexpression of miR-142-3p 
      remarkably inhibited PRRSV infection in PAMs, while blockage of endogenous 
      miR-142-3p significantly enhanced PRRSV replication. Then, we demonstrated that 
      miR-142-3p directly targeted Ras-related C3 botulinum toxin substrate 1 (Rac1), a 
      member of Rho GTPases family, by using luciferase reporter assay and UV 
      cross-linking and immunoprecipitation (CLIP) assay. Importantly, we verified that 
      miR-142-3p inhibited PRRSV entry into PAMs and accordingly suppressed PRRSV 
      infection by downregulating Rac1 expression. These findings reveal an important 
      role of miR-142-3p in modulating PRRSV infection and provide us with some ideas 
      for developing novel antiviral therapy against PRRSV infection.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Yao, Yao
AU  - Yao Y
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China 
      Agricultural University, Beijing 100193, China; Ministry of Agriculture Key 
      Laboratory of Soil Microbiology, College of Biological Sciences, China 
      Agricultural University, Beijing 100193, China; Department of Microbiology and 
      Immunology, College of Biological Sciences, China Agricultural University, 
      Beijing 100193, China.
FAU - Zhang, Xuan
AU  - Zhang X
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China 
      Agricultural University, Beijing 100193, China; Ministry of Agriculture Key 
      Laboratory of Soil Microbiology, College of Biological Sciences, China 
      Agricultural University, Beijing 100193, China; Department of Microbiology and 
      Immunology, College of Biological Sciences, China Agricultural University, 
      Beijing 100193, China.
FAU - Li, Sihan
AU  - Li S
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China 
      Agricultural University, Beijing 100193, China; Ministry of Agriculture Key 
      Laboratory of Soil Microbiology, College of Biological Sciences, China 
      Agricultural University, Beijing 100193, China; Department of Microbiology and 
      Immunology, College of Biological Sciences, China Agricultural University, 
      Beijing 100193, China.
FAU - Zhu, Yingqi
AU  - Zhu Y
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China 
      Agricultural University, Beijing 100193, China; Ministry of Agriculture Key 
      Laboratory of Soil Microbiology, College of Biological Sciences, China 
      Agricultural University, Beijing 100193, China; Department of Microbiology and 
      Immunology, College of Biological Sciences, China Agricultural University, 
      Beijing 100193, China.
FAU - Zheng, Xiaojie
AU  - Zheng X
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China 
      Agricultural University, Beijing 100193, China; Ministry of Agriculture Key 
      Laboratory of Soil Microbiology, College of Biological Sciences, China 
      Agricultural University, Beijing 100193, China; Department of Microbiology and 
      Immunology, College of Biological Sciences, China Agricultural University, 
      Beijing 100193, China.
FAU - Liu, Fang
AU  - Liu F
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China 
      Agricultural University, Beijing 100193, China; Ministry of Agriculture Key 
      Laboratory of Soil Microbiology, College of Biological Sciences, China 
      Agricultural University, Beijing 100193, China; Department of Microbiology and 
      Immunology, College of Biological Sciences, China Agricultural University, 
      Beijing 100193, China.
FAU - Feng, Wen-Hai
AU  - Feng WH
AD  - State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China 
      Agricultural University, Beijing 100193, China; Ministry of Agriculture Key 
      Laboratory of Soil Microbiology, College of Biological Sciences, China 
      Agricultural University, Beijing 100193, China; Department of Microbiology and 
      Immunology, College of Biological Sciences, China Agricultural University, 
      Beijing 100193, China. Electronic address: whfeng@cau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20220414
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Host-Pathogen Interactions/genetics
MH  - *MicroRNAs/metabolism
MH  - *Porcine Reproductive and Respiratory Syndrome
MH  - *Porcine respiratory and reproductive syndrome virus/genetics/metabolism
MH  - Swine
MH  - *Swine Diseases
MH  - Virus Replication/physiology
OTO - NOTNLM
OT  - Entry
OT  - MiR-142-3p
OT  - PRRSV
OT  - Rac1
EDAT- 2022/04/23 06:00
MHDA- 2022/05/25 06:00
CRDT- 2022/04/22 20:10
PHST- 2022/01/18 00:00 [received]
PHST- 2022/03/23 00:00 [revised]
PHST- 2022/04/10 00:00 [accepted]
PHST- 2022/04/23 06:00 [pubmed]
PHST- 2022/05/25 06:00 [medline]
PHST- 2022/04/22 20:10 [entrez]
AID - S0378-1135(22)00104-3 [pii]
AID - 10.1016/j.vetmic.2022.109434 [doi]
PST - ppublish
SO  - Vet Microbiol. 2022 Jun;269:109434. doi: 10.1016/j.vetmic.2022.109434. Epub 2022 
      Apr 14.