PMID- 35453544
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2227-9059 (Print)
IS  - 2227-9059 (Electronic)
IS  - 2227-9059 (Linking)
VI  - 10
IP  - 4
DP  - 2022 Mar 29
TI  - EGFR Amplification Is a Phenomenon of IDH Wildtype and TERT Mutated High-Grade 
      Glioma: An Integrated Analysis Using Fluorescence In Situ Hybridization and DNA 
      Methylome Profiling.
LID - 10.3390/biomedicines10040794 [doi]
LID - 794
AB  - Gliomas are the most common intrinsic brain tumors in adults, and in accordance 
      with their clinical behavior and patients' outcome, they are graded by the World 
      Health Organization (WHO) classification of brain tumors. One very interesting 
      candidate for targeted tumor therapy may be epidermal growth factor receptor 
      (EGFR) amplification. Here, we performed an integrated comparative analysis of 
      EGFR amplification in 34 glioma samples using standard fluorescence in situ 
      hybridization (FISH) and Illumina EPIC Infinium Methylation Bead Chip and 
      correlated results with molecular glioma hallmarks. We found that the EPIC 
      analysis showed the same power of detecting EGFR amplification compared with 
      FISH. EGFR amplification was detectable in high-grade gliomas (25%). Moreover, 
      EGFR amplification was found to be present solely in IDH wildtype gliomas (26%) 
      and TERT mutated gliomas (27%), occurring independently of MGMT promoter 
      methylation status and being mutually exclusive with 1p/19q codeletion (LOH). In 
      summary, EPIC Bead Chip analysis is a reliable tool for detecting EGFR 
      amplification and is comparable with the standard method FISH. EGFR amplification 
      is a phenomenon of IDH wildtype TERT mutated high-grade gliomas.
FAU - Holzl, Dorothee
AU  - Holzl D
AD  - Institute of Pathology, University Hospital Salzburg, Paracelsus Medical 
      University, Mullner Hauptstr. 48, A-5020 Salzburg, Austria.
FAU - Hutarew, Georg
AU  - Hutarew G
AD  - Institute of Pathology, University Hospital Salzburg, Paracelsus Medical 
      University, Mullner Hauptstr. 48, A-5020 Salzburg, Austria.
FAU - Zellinger, Barbara
AU  - Zellinger B
AD  - Institute of Pathology, University Hospital Salzburg, Paracelsus Medical 
      University, Mullner Hauptstr. 48, A-5020 Salzburg, Austria.
FAU - Alinger-Scharinger, Beate
AU  - Alinger-Scharinger B
AD  - Institute of Pathology, University Hospital Salzburg, Paracelsus Medical 
      University, Mullner Hauptstr. 48, A-5020 Salzburg, Austria.
FAU - Schlicker, Hans U
AU  - Schlicker HU
AD  - Institute of Pathology, University Hospital Salzburg, Paracelsus Medical 
      University, Mullner Hauptstr. 48, A-5020 Salzburg, Austria.
FAU - Schwartz, Christoph
AU  - Schwartz C
AD  - Department of Neurosurgery, University Hospital Salzburg, Paracelsus Medical 
      University, Ignaz-Harrer-Str. 79, A-5020 Salzburg, Austria.
FAU - Sotlar, Karl
AU  - Sotlar K
AD  - Institute of Pathology, University Hospital Salzburg, Paracelsus Medical 
      University, Mullner Hauptstr. 48, A-5020 Salzburg, Austria.
FAU - Kraus, Theo F J
AU  - Kraus TFJ
AUID- ORCID: 0000-0003-2944-5129
AD  - Institute of Pathology, University Hospital Salzburg, Paracelsus Medical 
      University, Mullner Hauptstr. 48, A-5020 Salzburg, Austria.
LA  - eng
PT  - Journal Article
DEP - 20220329
PL  - Switzerland
TA  - Biomedicines
JT  - Biomedicines
JID - 101691304
PMC - PMC9033057
OTO - NOTNLM
OT  - EGFR
OT  - EPIC DNA methylation analysis
OT  - FISH
OT  - glioblastoma
OT  - glioma
COIS- The authors declare no conflict of interest.
EDAT- 2022/04/24 06:00
MHDA- 2022/04/24 06:01
PMCR- 2022/03/29
CRDT- 2022/04/23 01:01
PHST- 2022/02/16 00:00 [received]
PHST- 2022/03/24 00:00 [revised]
PHST- 2022/03/25 00:00 [accepted]
PHST- 2022/04/23 01:01 [entrez]
PHST- 2022/04/24 06:00 [pubmed]
PHST- 2022/04/24 06:01 [medline]
PHST- 2022/03/29 00:00 [pmc-release]
AID - biomedicines10040794 [pii]
AID - biomedicines-10-00794 [pii]
AID - 10.3390/biomedicines10040794 [doi]
PST - epublish
SO  - Biomedicines. 2022 Mar 29;10(4):794. doi: 10.3390/biomedicines10040794.