PMID- 35453876
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220429
IS  - 2075-4418 (Print)
IS  - 2075-4418 (Electronic)
IS  - 2075-4418 (Linking)
VI  - 12
IP  - 4
DP  - 2022 Mar 28
TI  - Rapid and Reliable Detection of SARS-CoV-2 Using Direct RT-LAMP.
LID - 10.3390/diagnostics12040828 [doi]
LID - 828
AB  - Background: The global pandemic coronavirus SARS-CoV-2 has a healthcare, social 
      and economic burden. To limit the spread of the virus, the World Health 
      Organization (WHO) urgently called for extensive screening of suspected 
      individuals; thus, a quick, simple, and sensitive diagnostic assay is always in 
      need. Methods: We applied reverse transcription-loop-mediated isothermal 
      amplification (RT-LAMP) for the detection of SARS-CoV-2. The RT-LAMP method was 
      optimized by evaluating two fluorescence amplification mixes and several reaction 
      times, and results were compared to the standard real-time RT-PCR (rtRT-PCR). The 
      assay was validated using 200 nasopharyngeal swabs collected in viral transport 
      media (62 positive for SARS-CoV-2, and 138 negative for SARS-CoV-2 detected by 
      the rtRT-PCR method). The samples were diluted 1:4 in diethylpyrocarbonate 
      (DEPC)-treated water, utilized for RT-LAMP using different singleplex and 
      multiplex sets of LAMP primers (N gene, S gene, and orf1ab gene), and incubated 
      at 65  degrees C using real-time PCR 7500. Results: Our direct detection with the RT-LAMP 
      protocol showed 100% concordance (sensitivity and specificity) with the standard 
      protocol used for the detection of SARS-CoV-2 nucleic acid. Conclusions: In this 
      study, we set up a rapid, simple, and sensitive RT-LAMP assay for the detection 
      of SARS-CoV-2 in clinical samples. The assay is suitable for point of care 
      detection in public hospitals, medical centers in rural areas, and in 
      transportation hubs.
FAU - El-Kafrawy, Sherif A
AU  - El-Kafrawy SA
AUID- ORCID: 0000-0002-3667-7529
AD  - Special Infectious Agents Unit-BSL3, King Fahd Medical Research Center, King 
      Abdulaziz University, Jeddah 21589, Saudi Arabia.
AD  - Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, 
      King Abdulaziz University, Jeddah 21589, Saudi Arabia.
FAU - El-Daly, Mai M
AU  - El-Daly MM
AUID- ORCID: 0000-0002-3864-523X
AD  - Special Infectious Agents Unit-BSL3, King Fahd Medical Research Center, King 
      Abdulaziz University, Jeddah 21589, Saudi Arabia.
AD  - Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, 
      King Abdulaziz University, Jeddah 21589, Saudi Arabia.
FAU - Hassan, Ahmed M
AU  - Hassan AM
AD  - Special Infectious Agents Unit-BSL3, King Fahd Medical Research Center, King 
      Abdulaziz University, Jeddah 21589, Saudi Arabia.
FAU - Harakeh, Steve M
AU  - Harakeh SM
AD  - Special Infectious Agents Unit-BSL3, King Fahd Medical Research Center, King 
      Abdulaziz University, Jeddah 21589, Saudi Arabia.
AD  - Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, 
      King Abdulaziz University, Jeddah 21589, Saudi Arabia.
FAU - Alandijany, Thamir A
AU  - Alandijany TA
AUID- ORCID: 0000-0001-9130-4031
AD  - Special Infectious Agents Unit-BSL3, King Fahd Medical Research Center, King 
      Abdulaziz University, Jeddah 21589, Saudi Arabia.
AD  - Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, 
      King Abdulaziz University, Jeddah 21589, Saudi Arabia.
FAU - Azhar, Esam I
AU  - Azhar EI
AUID- ORCID: 0000-0002-1736-181X
AD  - Special Infectious Agents Unit-BSL3, King Fahd Medical Research Center, King 
      Abdulaziz University, Jeddah 21589, Saudi Arabia.
AD  - Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, 
      King Abdulaziz University, Jeddah 21589, Saudi Arabia.
LA  - eng
GR  - GCV19-48-1441/King Abdulaziz University/
PT  - Journal Article
DEP - 20220328
PL  - Switzerland
TA  - Diagnostics (Basel)
JT  - Diagnostics (Basel, Switzerland)
JID - 101658402
PMC - PMC9029081
OTO - NOTNLM
OT  - COVID-19
OT  - PCR
OT  - RT-LAMP
OT  - SARS-CoV-2
OT  - direct assay
OT  - molecular assay
COIS- The authors declare no conflict of interest.
EDAT- 2022/04/24 06:00
MHDA- 2022/04/24 06:01
PMCR- 2022/03/28
CRDT- 2022/04/23 01:02
PHST- 2022/03/06 00:00 [received]
PHST- 2022/03/26 00:00 [revised]
PHST- 2022/03/27 00:00 [accepted]
PHST- 2022/04/23 01:02 [entrez]
PHST- 2022/04/24 06:00 [pubmed]
PHST- 2022/04/24 06:01 [medline]
PHST- 2022/03/28 00:00 [pmc-release]
AID - diagnostics12040828 [pii]
AID - diagnostics-12-00828 [pii]
AID - 10.3390/diagnostics12040828 [doi]
PST - epublish
SO  - Diagnostics (Basel). 2022 Mar 28;12(4):828. doi: 10.3390/diagnostics12040828.