PMID- 35456131
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220429
IS  - 2076-0817 (Print)
IS  - 2076-0817 (Electronic)
IS  - 2076-0817 (Linking)
VI  - 11
IP  - 4
DP  - 2022 Apr 11
TI  - Antimicrobial Susceptibility of Microbiota in Bacterial Vaginosis Using 
      Fluorescence In Situ Hybridization.
LID - 10.3390/pathogens11040456 [doi]
LID - 456
AB  - BACKGROUND: Testing of antibiotic resistance of intact vaginal microbiota in pure 
      culture is not feasible. METHODS: Metronidazole, antiseptic octenisept((R)), 
      antimycotic ciclopirox, bacterial probiotic Lactobacillus crispatus, yeast 
      probiotic Saccharomyces boulardii, Gardnerella-phage-endolysin named phagolysin 
      and phagolysin in combination with probiotics were tested for bacteriolytic 
      activity. Included were vaginal swabs from 38 random women with Amsel-confirmed 
      bacterial vaginosis (BV). Test aliquots were incubated by 37 degrees  for 2 and 24 h. 
      Gardnerella, low G+C, Atopobium, lactobacilli, Lactobacillus iners and crispatus, 
      Prevotella-Bacteroides, and Gammaproteobacteria microbial groups were quantified 
      using fluorescence in situ hybridization (FISH). RESULTS: The probiotic strain 
      Lactobacillus crispatus demonstrated the weakest bacteriolytical effects, 
      followed by metronidazole. Both had no impact on Gardnerella species, instead 
      lysing Prevotella-Bacteroides, Enterobacteriaceae (by L.crispatus) or LGC, 
      Atopobium and Prevotella-Bacteroides (by metronidazole) groups of the microbiota. 
      Cytolytic activity on Gardnerella was highly pronounced and increased from 
      octenisept to ciclopirox, phagolysin, phagolysin with L.crispatus, being best in 
      the combination of phagolysin with S.boulardii. Universally active ciclopirox and 
      octenisept(R) suppressed nearly all microbial groups including those which are 
      regarded as beneficial. Phagolysin had no effect on naturally occurring 
      Lactobacillus crispatus. Conclusions: FISH susceptibility testing allows unique 
      efficacy evaluation of individually adjusted topical therapy without microbial 
      isolation facilitating optimal therapy choice.
FAU - Swidsinski, Alexander
AU  - Swidsinski A
AD  - Molecular-Genetic Laboratory for Polymicrobial Infections and Biofilms, Charite 
      CCM, Medizinische Klinik, Universitatsmedizin, 10117 Berlin, Germany.
AD  - Institute of Molecular Medicine, Sechenov First Moscow State Medical University, 
      119435 Moscow, Russia.
FAU - Guschin, Alexander
AU  - Guschin A
AUID- ORCID: 0000-0002-0399-1167
AD  - Moscow Scientific and Practical Center of Dermatovenerology and Cosmetology, 
      119071 Moscow, Russia.
FAU - Corsini, Lorenzo
AU  - Corsini L
AUID- ORCID: 0000-0002-8077-6559
AD  - BioNTech R&D (Austria) GmbH, Vienna Biocenter, 1110 Vienna, Austria.
FAU - Loening-Baucke, Vera
AU  - Loening-Baucke V
AD  - Molecular-Genetic Laboratory for Polymicrobial Infections and Biofilms, Charite 
      CCM, Medizinische Klinik, Universitatsmedizin, 10117 Berlin, Germany.
FAU - Tisakova, Lenka Podpera
AU  - Tisakova LP
AUID- ORCID: 0000-0002-2891-0888
AD  - BioNTech R&D (Austria) GmbH, Vienna Biocenter, 1110 Vienna, Austria.
FAU - Swidsinski, Sonja
AU  - Swidsinski S
AD  - MDI Limbach GmbH, Aroser Allee 84, 13407 Berlin, Germany.
FAU - Sobel, Jack D
AU  - Sobel JD
AD  - School of Medicine, Wayne State University, Detroit, MI 48201, USA.
FAU - Dorffel, Yvonne
AU  - Dorffel Y
AD  - Outpatient Clinic, Charite Universitatsmedizin Berlin, CCM, 10117 Berlin, 
      Germany.
LA  - eng
PT  - Journal Article
DEP - 20220411
PL  - Switzerland
TA  - Pathogens
JT  - Pathogens (Basel, Switzerland)
JID - 101596317
PMC - PMC9028502
OTO - NOTNLM
OT  - FISH
OT  - Gardnerella-phage-endolysin
OT  - Lactobacillus crispatus
OT  - Saccharomyces boulardii
OT  - antimicrobial resistance
OT  - bacterial vaginosis
OT  - dysbiosis
OT  - metronidazole
OT  - microbiome susceptibility
OT  - octenisept(R), ciclopirox
OT  - phagolysin
OT  - polymicrobials
COIS- The authors have no conflict of interest.
EDAT- 2022/04/24 06:00
MHDA- 2022/04/24 06:01
PMCR- 2022/04/11
CRDT- 2022/04/23 01:08
PHST- 2022/01/24 00:00 [received]
PHST- 2022/03/20 00:00 [revised]
PHST- 2022/04/04 00:00 [accepted]
PHST- 2022/04/23 01:08 [entrez]
PHST- 2022/04/24 06:00 [pubmed]
PHST- 2022/04/24 06:01 [medline]
PHST- 2022/04/11 00:00 [pmc-release]
AID - pathogens11040456 [pii]
AID - pathogens-11-00456 [pii]
AID - 10.3390/pathogens11040456 [doi]
PST - epublish
SO  - Pathogens. 2022 Apr 11;11(4):456. doi: 10.3390/pathogens11040456.