PMID- 35457035
OWN - NLM
STAT- MEDLINE
DCOM- 20220426
LR  - 20220716
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 23
IP  - 8
DP  - 2022 Apr 11
TI  - Differential Nanoscale Topography Dedicates Osteocyte-Manipulated Osteogenesis 
      via Regulation of the TGF-beta Signaling Pathway.
LID - 10.3390/ijms23084212 [doi]
LID - 4212
AB  - Osteocytes function as the master orchestrator of bone remodeling activity in the 
      telophase of osseointegration. However, most contemporary studies focus on the 
      manipulation of osteoblast and/or osteoclast functionality via implant surface 
      engineering, which neglects the pivotal role of osteocytes in de novo bone 
      formation. It is confirmative that osteocyte processes extend directly to the 
      implant surface, but whether the surface physicochemical properties can affect 
      the functionality of osteocytes and determine the fate of the osseointegration in 
      the final remodeling stage remains to be determined. Titania nanotube arrays 
      (NTAs) with distinct diameters were fabricated to investigate the relationship 
      between the nanoscale topography and the functionality of osteocytes. In vitro 
      results pinpointed that NTA with a diameter of 15 nm (NTA-15) significantly 
      promote osteogenesis of osteocytes via the enhancement of spreading, 
      proliferation, and mineralization. The osteocyte transcriptome of each group 
      further revealed that the TGF-beta signaling pathway plays a pivotal role in 
      osteocyte-mediated osteogenesis. The in vivo study definitely mirrored the 
      aforementioned results, that NTA-15 significantly promotes bone formation around 
      the implant. Consequently, nanoscaled topography-induced osteocyte functionality 
      is important in late osseointegration. This suggests that surface designs 
      targeting osteocytes may, therefore, be a potential approach to solving the 
      aseptic loosening of the implant, and thus strengthen osseointegration.
FAU - Cui, Jingyuan
AU  - Cui J
AD  - Key Laboratory for Ultrafine Materials of Ministry of Education, State Key 
      Laboratory of Bioreactor Engineering, East China University of Science and 
      Technology, Shanghai 200237, China.
AD  - Frontiers Science Center for Materiobiology and Dynamic Chemistry, College of 
      Materials Science and Engineering, East China University of Science and 
      Technology, Shanghai 200237, China.
FAU - Yang, Yaru
AU  - Yang Y
AD  - College of Materials and Textile Engineering, Jiaxing University, Jiaxing 314001, 
      China.
FAU - Chen, Peiru
AU  - Chen P
AD  - State Key Laboratory of Proteomics, Beijing Proteome Research Center, National 
      Center for Protein Sciences (Beijing), Institute of Lifeomics, Beijing 102206, 
      China.
FAU - Hang, Ruiqiang
AU  - Hang R
AD  - Shanxi Key Laboratory of Biomedical Metal Materials, College of Materials Science 
      and Engineering, Taiyuan University of Technology, Taiyuan 030024, China.
FAU - Xiao, Yin
AU  - Xiao Y
AUID- ORCID: 0000-0003-1785-3491
AD  - Australia-China Centre for Tissue Engineering and Regenerative Medicine, 
      Queensland University of Technology, Brisbane, QLD 4000, Australia.
FAU - Liu, Xueting
AU  - Liu X
AD  - Key Laboratory for Ultrafine Materials of Ministry of Education, State Key 
      Laboratory of Bioreactor Engineering, East China University of Science and 
      Technology, Shanghai 200237, China.
FAU - Zhang, Lixin
AU  - Zhang L
AD  - Key Laboratory for Ultrafine Materials of Ministry of Education, State Key 
      Laboratory of Bioreactor Engineering, East China University of Science and 
      Technology, Shanghai 200237, China.
FAU - Sun, Hui
AU  - Sun H
AD  - Department of Orthopedics, Shanghai Jiaotong University School of Medicine, 
      Shanghai 200233, China.
FAU - Bai, Long
AU  - Bai L
AUID- ORCID: 0000-0002-4757-746X
AD  - Key Laboratory for Ultrafine Materials of Ministry of Education, State Key 
      Laboratory of Bioreactor Engineering, East China University of Science and 
      Technology, Shanghai 200237, China.
AD  - Frontiers Science Center for Materiobiology and Dynamic Chemistry, College of 
      Materials Science and Engineering, East China University of Science and 
      Technology, Shanghai 200237, China.
LA  - eng
GR  - 81901898/National Natural Science Foundation of China/
GR  - 2019M661404/China Postdoctoral Science Foundation/
GR  - 2020T130192/China Postdoctoral Science Foundation/
PT  - Journal Article
DEP - 20220411
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Osteoblasts/metabolism
MH  - *Osteocytes/metabolism
MH  - *Osteogenesis
MH  - Signal Transduction
MH  - Transforming Growth Factor beta/metabolism
PMC - PMC9027032
OTO - NOTNLM
OT  - TGF-beta
OT  - nanoscale
OT  - osseointegration
OT  - osteocyte
OT  - osteogenesis
COIS- The authors declare no conflict of interest.
EDAT- 2022/04/24 06:00
MHDA- 2022/04/27 06:00
PMCR- 2022/04/11
CRDT- 2022/04/23 01:11
PHST- 2022/02/03 00:00 [received]
PHST- 2022/03/31 00:00 [revised]
PHST- 2022/04/07 00:00 [accepted]
PHST- 2022/04/23 01:11 [entrez]
PHST- 2022/04/24 06:00 [pubmed]
PHST- 2022/04/27 06:00 [medline]
PHST- 2022/04/11 00:00 [pmc-release]
AID - ijms23084212 [pii]
AID - ijms-23-04212 [pii]
AID - 10.3390/ijms23084212 [doi]
PST - epublish
SO  - Int J Mol Sci. 2022 Apr 11;23(8):4212. doi: 10.3390/ijms23084212.