PMID- 35460895
OWN - NLM
STAT- MEDLINE
DCOM- 20220524
LR  - 20220714
IS  - 1531-4332 (Electronic)
IS  - 1095-6433 (Linking)
VI  - 269
DP  - 2022 Jul
TI  - Ammonia transporter 2 as a molecular marker to elucidate the potentials of 
      ammonia transport in phylotypes of Symbiodinium, Cladocopium and Durusdinium in 
      the fluted giant clam, Tridacna squamosa.
PG  - 111225
LID - S1095-6433(22)00083-6 [pii]
LID - 10.1016/j.cbpa.2022.111225 [doi]
AB  - Giant clams harbor coccoid Symbiodiniaceae dinoflagellates that are phototrophic. 
      These dinoflagellates generally include multiple phylotypes (species) of 
      Symbiodinium, Cladocopium, and Durusdinium in disparate proportions depending on 
      the environmental conditions. The coccoid symbionts can share photosynthate with 
      the clam host, which in return supply them with nutrients containing inorganic 
      carbon, nitrogen and phosphorus. Symbionts can recycle nitrogen by absorbing and 
      assimilating the endogenous ammonia produced by the host. This study aimed to use 
      the transcript levels of ammonia transporter 2 (AMT2) in Symbiodinium 
      (Symb-AMT2), Cladocopium (Clad-AMT2) and Durusdinium (Duru-AMT2) as molecular 
      indicators to estimate the potential of ammonia transport in these three genera 
      of Symbiodiniaceae dinoflagellates in different organs of the fluted giant clam, 
      Tridacna squamosa, obtained from Vietnam. We also determined the transcript 
      levels of form II ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcII) and 
      nitrate transporter 2 (NRT2) in Symbiodinium (Symb-rbcII; Symb-NRT2), Cladocopium 
      (Clad-rbcII; Clad-NRT2) and Durusdinium (Duru-rbcII; Duru-NRT2), in order to 
      examine the potential of ammonia transport with reference to the potentials of 
      phototrophy or NO(3)(-) uptake independent of the quantities and proportion of 
      these Symbiodiniaceae phylotypes. Our results indicated for the first time that 
      phylotypes of Symbiodinium and Cladocopium could have different potentials of 
      ammonia transport, and that phylotypes of Symbiodinium might have higher 
      potential of NO(3)(-) transport than ammonia transport. They also suggested that 
      Symbiodiniaceae phylotypes residing in different organs of T. squamosa could have 
      disparate potentials of ammonia transport, alluding to the functional diversity 
      among phylotypes of coccoid Symbiodinium, Cladocopium, and Durusdinium.
CI  - Copyright (c) 2022 Elsevier Inc. All rights reserved.
FAU - Pang, Caryn Z
AU  - Pang CZ
AD  - Natural Sciences and Science Education, National Institute of Education, Nanyang 
      Technological University, 1 Nanyang Walk, Singapore 637616, Republic of 
      Singapore.
FAU - Ip, Yuen K
AU  - Ip YK
AD  - Department of Biological Sciences, National University of Singapore, Kent Ridge, 
      Singapore 117543, Republic of Singapore.
FAU - Chew, Shit F
AU  - Chew SF
AD  - Natural Sciences and Science Education, National Institute of Education, Nanyang 
      Technological University, 1 Nanyang Walk, Singapore 637616, Republic of 
      Singapore. Electronic address: sfun.chew@nie.edu.sg.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220420
PL  - United States
TA  - Comp Biochem Physiol A Mol Integr Physiol
JT  - Comparative biochemistry and physiology. Part A, Molecular & integrative 
      physiology
JID - 9806096
RN  - 0 (Membrane Transport Proteins)
RN  - 7664-41-7 (Ammonia)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Ammonia/metabolism
MH  - Animals
MH  - *Anthozoa/metabolism
MH  - *Bivalvia/metabolism
MH  - *Dinoflagellida/physiology
MH  - Membrane Transport Proteins
MH  - Nitrogen
MH  - Symbiosis
OTO - NOTNLM
OT  - Amino acids
OT  - Coral reefs
OT  - Photosynthate
OT  - Symbiodiniaceae dinoflagellates
OT  - Symbiosis
OT  - Zooxanthellae
EDAT- 2022/04/24 06:00
MHDA- 2022/05/25 06:00
CRDT- 2022/04/23 20:10
PHST- 2021/11/15 00:00 [received]
PHST- 2022/04/17 00:00 [revised]
PHST- 2022/04/17 00:00 [accepted]
PHST- 2022/04/24 06:00 [pubmed]
PHST- 2022/05/25 06:00 [medline]
PHST- 2022/04/23 20:10 [entrez]
AID - S1095-6433(22)00083-6 [pii]
AID - 10.1016/j.cbpa.2022.111225 [doi]
PST - ppublish
SO  - Comp Biochem Physiol A Mol Integr Physiol. 2022 Jul;269:111225. doi: 
      10.1016/j.cbpa.2022.111225. Epub 2022 Apr 20.