PMID- 35462137
OWN - NLM
STAT- MEDLINE
DCOM- 20220530
LR  - 20220530
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 245
DP  - 2022 Aug 1
TI  - Triple chemical derivatization strategy assisted liquid chromatography-mass 
      spectrometry for determination of retinoic acids in human serum.
PG  - 123474
LID - S0039-9140(22)00270-3 [pii]
LID - 10.1016/j.talanta.2022.123474 [doi]
AB  - Retinoic acids (RAs), an important class of fatty acid derived from vitamin A, 
      are closely associated with various human diseases including cancer. Hence, 
      determination of endogenous RAs would help to uncover the mechanisms underlying 
      RAs-related diseases. However, accurate quantification of RAs is still a 
      challenge due to their high structure similarity, low abundance in biological 
      samples and the lack of isotope internal standards (ISs). In this study, a liquid 
      chromatography-mass spectrometry (LC-MS) method with high-sensitivity and 
      high-throughput was developed to simultaneously determine 5 RAs 
      (all-trans-retinoic acid, 13-cis-retinoic acid, 9,13-cis-retinoic acid, 
      all-trans-4-oxoretinoic acid and 4-hydroxy-retinoic acid) in human serum. In the 
      method, three derivatization reagents, N, N-dimethylethylenediamine (DMED), 
      d(4)-N,N-dimethylethylenediamine (d(4)-DMED) and 
      (13)C(2)-N,N-dimethylethylenediamine ((13)C(2)-DMED), were used for triple 
      chemical derivatization of RAs, thus the detection sensitivity and analysis 
      throughput of endogenous RAs could be achieved. Benefiting from the developed 
      strategy, the analysis throughput was enhanced and the detection sensitivity of 
      RAs was increased by 14-398 folds. The limits of quantification (LOQs) of RAs 
      were found to be between 8.4 and 130 pg/mL, which were better than previously 
      reported methods. Good linearities of RAs were obtained with determination 
      coefficient (R(2)) ranging from 0.9774 to 0.9999. The intra- and inter-day 
      relative standard deviations (RSDs) were below 11.7% and 12.7%, respectively, 
      indicating the acceptable reproducibility of the method. Using the developed 
      method, we successfully quantified 4 RAs (all-trans-retinoic acid, 
      13-cis-retinoic acid, 9,13-cis-retinoic acid and all-trans-4-oxoretinoic acid) in 
      health controls and cancer patient serum samples. Furthermore, t-test analysis 
      showed that the concentration of three RAs (all-trans-retinoic acid, 
      13-cis-retinoic acid and all-trans-4-oxoretinoic acid) in cancer patient serum 
      samples were significantly decreased compared with health controls, which 
      indicated that RAs might play an important role in the formation and development 
      of cancer.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Gong, Ge-Ge
AU  - Gong GG
AD  - Department of Chemistry, Wuhan University, Wuhan, 430072, PR China.
FAU - Zheng, Jie
AU  - Zheng J
AD  - Singapore Phenome Center, Lee Kong Chian School of Medicine, Nanyang 
      Technological University, Singapore.
FAU - Li, Sha
AU  - Li S
AD  - Department of Chemistry, Wuhan University, Wuhan, 430072, PR China.
FAU - Bai, Ya-Li
AU  - Bai YL
AD  - Department of Chemistry, Wuhan University, Wuhan, 430072, PR China.
FAU - Feng, Yu-Qi
AU  - Feng YQ
AD  - Department of Chemistry, Wuhan University, Wuhan, 430072, PR China; School of 
      Public Health, Wuhan University, Wuhan, 430071, PR China. Electronic address: 
      yqfeng@whu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20220416
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 5688UTC01R (Tretinoin)
RN  - EH28UP18IF (Isotretinoin)
SB  - IM
MH  - Chromatography, High Pressure Liquid/methods
MH  - Chromatography, Liquid
MH  - Humans
MH  - *Isotretinoin
MH  - Mass Spectrometry
MH  - Reproducibility of Results
MH  - *Tretinoin
OTO - NOTNLM
OT  - Accurate quantification
OT  - Derivatization
OT  - Liquid chromatography-mass spectrometry
OT  - Retinoic acid
EDAT- 2022/04/25 06:00
MHDA- 2022/05/31 06:00
CRDT- 2022/04/24 20:24
PHST- 2022/01/29 00:00 [received]
PHST- 2022/04/06 00:00 [revised]
PHST- 2022/04/09 00:00 [accepted]
PHST- 2022/04/25 06:00 [pubmed]
PHST- 2022/05/31 06:00 [medline]
PHST- 2022/04/24 20:24 [entrez]
AID - S0039-9140(22)00270-3 [pii]
AID - 10.1016/j.talanta.2022.123474 [doi]
PST - ppublish
SO  - Talanta. 2022 Aug 1;245:123474. doi: 10.1016/j.talanta.2022.123474. Epub 2022 Apr 
      16.