PMID- 35463981
OWN - NLM
STAT- MEDLINE
DCOM- 20220426
LR  - 20240225
IS  - 2314-6141 (Electronic)
IS  - 2314-6133 (Print)
VI  - 2022
DP  - 2022
TI  - Effects of Lipolysis-Stimulated Lipoprotein Receptor on Tight Junctions of 
      Pancreatic Ductal Epithelial Cells in Hypertriglyceridemic Acute Pancreatitis.
PG  - 4234186
LID - 10.1155/2022/4234186 [doi]
LID - 4234186
AB  - OBJECTIVE: We investigated the effects of lipolysis-stimulated lipoprotein 
      receptor (LSR) on the tight junctions (TJs) of pancreatic ductal epithelial cells 
      (PDECs) in hypertriglyceridemic acute pancreatitis (HTGAP). METHODS: 
      Sprague-Dawley rats were fed standard rat chow or a high-fat diet and injected 
      with sodium taurocholate to obtain normal and HTGAP rats, respectively. Serum 
      triglyceride (TG) levels, pathological changes, TJ proteins in the pancreas, and 
      TJ ultrastructure of PDECs were assessed. LSR overexpression (OE) and knockdown 
      (KD) HPDE6-C7 models were designed and cultured in a high-fat environment. 
      Protein levels were quantified by Western blotting. Cell monolayer permeability 
      was detected using FITC-Dextran. RESULTS: Serum TG concentration and pancreatic 
      scores were higher in the HTGAP group than in the normal group. Among the TJ 
      proteins, LSR protein expression was significantly lower in the HTGAP group than 
      in the acute pancreatitis (AP) group. Tricellulin (TRIC) expression in the 
      pancreatic ductal epithelia was higher in the HTGAP group than in the AP group. 
      The HTGAP group had lower TJ protein levels, wider intercellular space, and 
      widespread cellular necrosis with disappearance of cell junction structures. In 
      the cell study, TJ proteins were downregulated and the cellular barrier was 
      impaired by palmitic acid (PA), which was reversed by LSR-OE, whereas LSR-KD 
      downregulated the TJ proteins and aggravated PA-induced cellular barrier 
      impairment. CONCLUSIONS: Hypertriglyceridemia downregulates the TJ proteins in 
      PDECs, which may impair the pancreatic ductal mucosal barrier function. LSR 
      regulation can change the effects of HTG on cellular barrier function by 
      upregulating the TJ proteins.
CI  - Copyright (c) 2022 Jie Wang et al.
FAU - Wang, Jie
AU  - Wang J
AUID- ORCID: 0000-0002-0106-7843
AD  - Department of Gastroenterology, The Second Affiliated Hospital of Guangxi Medical 
      University, Nanning, China.
FAU - Qin, Mengbin
AU  - Qin M
AUID- ORCID: 0000-0001-9927-5482
AD  - Department of Gastroenterology, The Second Affiliated Hospital of Guangxi Medical 
      University, Nanning, China.
FAU - Wu, Qing
AU  - Wu Q
AUID- ORCID: 0000-0002-8252-2990
AD  - Department of Gastroenterology, The Second Affiliated Hospital of Guangxi Medical 
      University, Nanning, China.
FAU - Yang, Huiying
AU  - Yang H
AUID- ORCID: 0000-0002-2185-780X
AD  - Department of Gastroenterology, The First Affiliated Hospital of Guangxi Medical 
      University, Nanning, China.
FAU - Wei, Biwei
AU  - Wei B
AUID- ORCID: 0000-0002-8014-3911
AD  - Department of Gastroenterology, The First Affiliated Hospital of Guangxi Medical 
      University, Nanning, China.
FAU - Xie, Jinlian
AU  - Xie J
AUID- ORCID: 0000-0003-1936-371X
AD  - Department of Gastroenterology, The First Affiliated Hospital of Guangxi Medical 
      University, Nanning, China.
FAU - Qin, Yingying
AU  - Qin Y
AUID- ORCID: 0000-0002-5496-0854
AD  - Department of Gastroenterology, The First Affiliated Hospital of Guangxi Medical 
      University, Nanning, China.
FAU - Liang, Zhihai
AU  - Liang Z
AUID- ORCID: 0000-0001-5348-0130
AD  - Department of Gastroenterology, The First Affiliated Hospital of Guangxi Medical 
      University, Nanning, China.
FAU - Huang, Jiean
AU  - Huang J
AUID- ORCID: 0000-0003-0431-1888
AD  - Department of Gastroenterology, The Second Affiliated Hospital of Guangxi Medical 
      University, Nanning, China.
LA  - eng
PT  - Journal Article
DEP - 20220414
PL  - United States
TA  - Biomed Res Int
JT  - BioMed research international
JID - 101600173
RN  - 0 (Lsr protein, rat)
RN  - 0 (Receptors, LDL)
RN  - 0 (Receptors, Lipoprotein)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - Epithelial Cells/metabolism/pathology
MH  - Hypertriglyceridemia/complications/metabolism
MH  - Lipolysis
MH  - *Pancreatitis/etiology/genetics/metabolism/pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, LDL/genetics/metabolism
MH  - *Receptors, Lipoprotein/genetics/metabolism
MH  - Tight Junctions/metabolism
MH  - Transcription Factors/genetics/metabolism
PMC - PMC9023160
COIS- The authors declare that they have no conflicts of interest.
EDAT- 2022/04/26 06:00
MHDA- 2022/04/27 06:00
PMCR- 2022/04/14
CRDT- 2022/04/25 05:26
PHST- 2021/11/04 00:00 [received]
PHST- 2022/03/17 00:00 [revised]
PHST- 2022/04/04 00:00 [accepted]
PHST- 2022/04/25 05:26 [entrez]
PHST- 2022/04/26 06:00 [pubmed]
PHST- 2022/04/27 06:00 [medline]
PHST- 2022/04/14 00:00 [pmc-release]
AID - 10.1155/2022/4234186 [doi]
PST - epublish
SO  - Biomed Res Int. 2022 Apr 14;2022:4234186. doi: 10.1155/2022/4234186. eCollection 
      2022.