PMID- 35464363
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220429
IS  - 2297-1769 (Print)
IS  - 2297-1769 (Electronic)
IS  - 2297-1769 (Linking)
VI  - 9
DP  - 2022
TI  - Phenotypic Characterization of a Virulent PRRSV-1 Isolate in a Reproductive Model 
      With and Without Prior Heterologous Modified Live PRRSV-1 Vaccination.
PG  - 820233
LID - 10.3389/fvets.2022.820233 [doi]
LID - 820233
AB  - Reproductive disorders induced by porcine reproductive and respiratory syndrome 
      virus (PRRSV) cause high economic losses in the pig industry worldwide. In this 
      study, we aimed to phenotypically characterize a virulent PRRSV-1 subtype 1 
      isolate (AUT15-33) in a reproductive model. Furthermore, the protective effect of 
      a heterologous modified live virus vaccine (ReproCyc(R) PRRS EU) was evaluated. In 
      addition, PRRSV AUT15-33 was genotypically compared to other well-characterized 
      isolates. Sixteen gilts were equally divided into four groups: a vaccinated and 
      infected group (V-I), a vaccinated and non-infected group (V-NI), a 
      non-vaccinated and infected group (NV-I), and a non-vaccinated and non-infected 
      (NV-NI) group. After PRRSV infection on gestation day 84, all gilts were 
      clinically examined on a daily basis, and blood samples were taken at five 
      timepoints. Necropsy was performed 3 weeks after infection. The fetal 
      preservation status was assessed, and PRRSV RNA concentrations were measured in 
      the blood and tissue samples from all gilts and fetuses. After infection, all 
      four gilts in the NV-I group were viremic throughout 17 days post-infection 
      (dpi), whereas two gilts in the V-I group were viremic at only one timepoint at 6 
      dpi. The viral load was significantly higher in gilt serum, tracheobronchial 
      lymph nodes, uterine lymph nodes, maternal endometrium, and fetal placenta of 
      NV-I gilts compared to the V-I ones (p < 0.05). Moreover, the preservation status 
      of the fetuses derived from NV-I gilts was significantly impaired (55.9% of 
      viable fetuses) compared to the other groups (p < 0.001). Upon comparison with 
      other known isolates, the phylogenetic analyses revealed the closest relation to 
      a well-characterized PRRSV-1 subtype 1 field isolate from Belgium. In conclusion, 
      the high virulence of AUT15-33 was phenotypically confirmed in an experimental 
      reproductive model. The vaccination of the gilts showed promising results in 
      reducing viremia, fetal damage, and transplacental transmission of the PRRSV-1 
      strain characterized in this study.
CI  - Copyright (c) 2022 Kreutzmann, Stadler, Knecht, Sassu, Ruczizka, Zablotski, Vatzia, 
      Balka, Zaruba, Chen, Riedel, Rumenapf and Ladinig.
FAU - Kreutzmann, Heinrich
AU  - Kreutzmann H
AD  - Department for Farm Animals and Veterinary Public Health, University Clinic for 
      Swine, University of Veterinary Medicine Vienna, Vienna, Austria.
FAU - Stadler, Julia
AU  - Stadler J
AD  - Clinic for Swine, Centre for Clinical Veterinary Medicine, 
      Ludwig-Maximilians-University Munich, Oberschleissheim, Germany.
FAU - Knecht, Christian
AU  - Knecht C
AD  - Department for Farm Animals and Veterinary Public Health, University Clinic for 
      Swine, University of Veterinary Medicine Vienna, Vienna, Austria.
FAU - Sassu, Elena L
AU  - Sassu EL
AD  - Department for Farm Animals and Veterinary Public Health, University Clinic for 
      Swine, University of Veterinary Medicine Vienna, Vienna, Austria.
FAU - Ruczizka, Ursula
AU  - Ruczizka U
AD  - Department for Farm Animals and Veterinary Public Health, University Clinic for 
      Swine, University of Veterinary Medicine Vienna, Vienna, Austria.
FAU - Zablotski, Yury
AU  - Zablotski Y
AD  - Clinic for Swine, Centre for Clinical Veterinary Medicine, 
      Ludwig-Maximilians-University Munich, Oberschleissheim, Germany.
FAU - Vatzia, Eleni
AU  - Vatzia E
AD  - Department of Pathobiology, Institute of Immunology, University of Veterinary 
      Medicine Vienna, Vienna, Austria.
FAU - Balka, Gyula
AU  - Balka G
AD  - Department of Pathology, University of Veterinary Medicine Budapest, Budapest, 
      Hungary.
FAU - Zaruba, Marianne
AU  - Zaruba M
AD  - Department of Pathobiology, Institute of Virology, University of Veterinary 
      Medicine Vienna, Vienna, Austria.
FAU - Chen, Hann-Wei
AU  - Chen HW
AD  - Department of Pathobiology, Institute of Virology, University of Veterinary 
      Medicine Vienna, Vienna, Austria.
FAU - Riedel, Christiane
AU  - Riedel C
AD  - Department of Pathobiology, Institute of Virology, University of Veterinary 
      Medicine Vienna, Vienna, Austria.
FAU - Rumenapf, Till
AU  - Rumenapf T
AD  - Department of Pathobiology, Institute of Virology, University of Veterinary 
      Medicine Vienna, Vienna, Austria.
FAU - Ladinig, Andrea
AU  - Ladinig A
AD  - Department for Farm Animals and Veterinary Public Health, University Clinic for 
      Swine, University of Veterinary Medicine Vienna, Vienna, Austria.
LA  - eng
PT  - Journal Article
DEP - 20220407
PL  - Switzerland
TA  - Front Vet Sci
JT  - Frontiers in veterinary science
JID - 101666658
PMC - PMC9022457
OTO - NOTNLM
OT  - AUT15-33
OT  - PRRSV-1
OT  - PRRSV-1 phenotypic characterization
OT  - ReproCyc(R) PRRS EU
OT  - experimental reproductive model
OT  - modified live virus vaccine
OT  - porcine reproductive and respiratory syndrome virus (PRRSV)
OT  - vaccine efficacy
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/04/26 06:00
MHDA- 2022/04/26 06:01
PMCR- 2022/01/01
CRDT- 2022/04/25 05:32
PHST- 2021/11/22 00:00 [received]
PHST- 2022/02/07 00:00 [accepted]
PHST- 2022/04/25 05:32 [entrez]
PHST- 2022/04/26 06:00 [pubmed]
PHST- 2022/04/26 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fvets.2022.820233 [doi]
PST - epublish
SO  - Front Vet Sci. 2022 Apr 7;9:820233. doi: 10.3389/fvets.2022.820233. eCollection 
      2022.