PMID- 35468250
OWN - NLM
STAT- MEDLINE
DCOM- 20220602
LR  - 20220716
IS  - 1552-4930 (Electronic)
IS  - 1552-4922 (Print)
IS  - 1552-4922 (Linking)
VI  - 101
IP  - 6
DP  - 2022 Jun
TI  - Simultaneous analysis of antigen-specific B and T cells after SARS-CoV-2 
      infection and vaccination.
PG  - 474-482
LID - 10.1002/cyto.a.24563 [doi]
AB  - Conventional methods for quantifying and phenotyping antigen-specific lymphocytes 
      can rapidly deplete irreplaceable specimens. This is due to the fact that 
      antigen-specific T and B cells have historically been analyzed in independent 
      assays each requiring millions of cells. A technique that facilitates the 
      simultaneous detection of antigen-specific T and B cells would allow for more 
      thorough immune profiling with significantly reduced sample requirements. To this 
      end, we developed the B and T cell tandem lymphocyte evaluation (BATTLE) assay, 
      which allows for the simultaneous identification of SARS-CoV-2 Spike reactive T 
      and B cells using an activation induced marker (AIM) T cell assay and dual-color 
      B cell antigen probes. Using this assay, we demonstrate that antigen-specific B 
      and T cell subsets can be identified simultaneously using conventional flow 
      cytometry platforms and provide insight into the differential effects of mRNA 
      vaccination on B and T cell populations following natural SARS-CoV-2 infection.
CI  - (c) 2022 The Authors. Cytometry Part A published by Wiley Periodicals LLC on behalf 
      of International Society for Advancement of Cytometry.
FAU - Newell, Krista L
AU  - Newell KL
AUID- ORCID: 0000-0002-2152-2073
AD  - Department of Microbiology and Immunology, SUNY Upstate Medical University, 
      Syracuse, New York, USA.
FAU - Waldran, Mitchell J
AU  - Waldran MJ
AD  - Department of Microbiology and Immunology, SUNY Upstate Medical University, 
      Syracuse, New York, USA.
FAU - Thomas, Stephen J
AU  - Thomas SJ
AD  - Department of Microbiology and Immunology, SUNY Upstate Medical University, 
      Syracuse, New York, USA.
AD  - Institute for Global Health and Translational Science, SUNY Upstate Medical 
      University, Syracuse, New York, USA.
FAU - Endy, Timothy P
AU  - Endy TP
AD  - Department of Microbiology and Immunology, SUNY Upstate Medical University, 
      Syracuse, New York, USA.
FAU - Waickman, Adam T
AU  - Waickman AT
AUID- ORCID: 0000-0002-4536-6714
AD  - Department of Microbiology and Immunology, SUNY Upstate Medical University, 
      Syracuse, New York, USA.
AD  - Institute for Global Health and Translational Science, SUNY Upstate Medical 
      University, Syracuse, New York, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220505
PL  - United States
TA  - Cytometry A
JT  - Cytometry. Part A : the journal of the International Society for Analytical 
      Cytology
JID - 101235694
SB  - IM
MH  - B-Lymphocytes
MH  - *COVID-19
MH  - Humans
MH  - SARS-CoV-2
MH  - T-Lymphocytes
MH  - Vaccination
PMC - PMC9087145
OTO - NOTNLM
OT  - B cells
OT  - SARS-CoV-2
OT  - T cells
OT  - antigen-specific
OT  - natural infection
OT  - spike protein
OT  - vaccination
COIS- Stephen J. Thomas reports compensation from Pfizer, during the conduct of the 
      study; personal fees from Merck, Sanofi, Takeda, Themisbio, and Janssen, outside 
      the submitted work. All other authors declare that the research was conducted in 
      the absence of any commercial or financial relationships that could be construed 
      as a potential conflict of interest.
EDAT- 2022/04/26 06:00
MHDA- 2022/06/03 06:00
PMCR- 2022/05/05
CRDT- 2022/04/25 17:25
PHST- 2022/02/10 00:00 [revised]
PHST- 2021/12/11 00:00 [received]
PHST- 2022/04/22 00:00 [accepted]
PHST- 2022/04/26 06:00 [pubmed]
PHST- 2022/06/03 06:00 [medline]
PHST- 2022/04/25 17:25 [entrez]
PHST- 2022/05/05 00:00 [pmc-release]
AID - CYTOA24563 [pii]
AID - 10.1002/cyto.a.24563 [doi]
PST - ppublish
SO  - Cytometry A. 2022 Jun;101(6):474-482. doi: 10.1002/cyto.a.24563. Epub 2022 May 5.