PMID- 35469160
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 1741-427X (Print)
IS  - 1741-4288 (Electronic)
IS  - 1741-427X (Linking)
VI  - 2022
DP  - 2022
TI  - Network Pharmacology-Based Prediction and Verification of the Potential Targets 
      of Pinoresinol Diglucoside for OA Treatment.
PG  - 9733742
LID - 10.1155/2022/9733742 [doi]
LID - 9733742
AB  - OBJECTIVE: This study aimed to explore the effects and related mechanisms of 
      pinoresinol diglucoside (PDG) on osteoarthritis (OA) via a combination of 
      pharmacology and animal experiments. METHODS: Traditional Chinese Medicine 
      Database and Analysis Platform (TCMSP) Drugbank, Online Mendelian Inheritance in 
      Man, and GeneCards databases were used to predict the putative targets of PGD 
      against OA. A protein protein interaction (PPI) network was constructed in STING 
      database to analyze the interaction network of these targets. Enrichment analysis 
      was performed with DAVID database. The OA model was built by anterior cruciate 
      ligament transection and then injected with PDG for 5 weeks. Hematoxylin and 
      eosin (HE) staining and safranin-fixed green staining were used to evaluate the 
      pathological change. ELISA was applied to measure the serum levels of 
      interleukin-1beta (IL-1beta), interleukin-6 (IL-6), and tumor necrosis factor-alpha 
      (TNF-alpha). Immunohistochemistry was employed to detect the protein levels of kinase 
      B (AKT), BAX, Bcl2, matrix metalloproteinase-1 (MMP-1), tissue inhibitor of 
      metalloproteinases 1 (TIMP-1), and phosphatidylinositol 3 kinase (PI3K) in knee 
      cartilage tissues. RESULTS: Seventy-one key targets were identified, including 
      AKT1, epidermal growth factor receptor, SRC, estrogen receptor 1 (ESR1), and 
      MMP9. Enrichment analysis revealed a series of pathway related to cancer, 
      PI3K-Akt signaling pathway, and proteoglycans in cancer. Animal experiments 
      showed that PDG alleviated the abnormal histomorphological changes of OA; 
      suppressed TIPM, serum IL-1beta, IL-6, and TNF-alpha levels, and PI3K and AKT 
      activation; and increased MMP-1 expression and Bcl2/Bax ratio. CONCLUSION: PDG 
      has a cartilage-protecting effect associated with PI3K/AKT signaling pathway in 
      rabbit with OA and therefore might serve as a potential agent for the treatment 
      of this disease.
CI  - Copyright (c) 2022 Hongkan Lou et al.
FAU - Lou, Hongkan
AU  - Lou H
AUID- ORCID: 0000-0001-8241-6832
AD  - Ningbo Hospital of Traditional Chinese Medicine Affiliated to Zhejiang Chinese 
      Medical University, Ningbo 315012, China.
FAU - Zhang, Yang
AU  - Zhang Y
AD  - Zhejiang Academy of Traditional Chinese Medicine, Hangzhou 310000, China.
FAU - Fang, Jianli
AU  - Fang J
AD  - Ningbo Hospital of Traditional Chinese Medicine Affiliated to Zhejiang Chinese 
      Medical University, Ningbo 315012, China.
FAU - Jin, Yong
AU  - Jin Y
AUID- ORCID: 0000-0003-2586-7594
AD  - Ningbo Hospital of Traditional Chinese Medicine Affiliated to Zhejiang Chinese 
      Medical University, Ningbo 315012, China.
LA  - eng
PT  - Journal Article
DEP - 20220416
PL  - United States
TA  - Evid Based Complement Alternat Med
JT  - Evidence-based complementary and alternative medicine : eCAM
JID - 101215021
PMC - PMC9034917
COIS- The authors declare that they have no conflicts of interest.
EDAT- 2022/04/27 06:00
MHDA- 2022/04/27 06:01
PMCR- 2022/04/16
CRDT- 2022/04/26 05:30
PHST- 2021/10/26 00:00 [received]
PHST- 2022/03/16 00:00 [revised]
PHST- 2022/03/17 00:00 [accepted]
PHST- 2022/04/26 05:30 [entrez]
PHST- 2022/04/27 06:00 [pubmed]
PHST- 2022/04/27 06:01 [medline]
PHST- 2022/04/16 00:00 [pmc-release]
AID - 10.1155/2022/9733742 [doi]
PST - epublish
SO  - Evid Based Complement Alternat Med. 2022 Apr 16;2022:9733742. doi: 
      10.1155/2022/9733742. eCollection 2022.