PMID- 35472469
OWN - NLM
STAT- MEDLINE
DCOM- 20220524
LR  - 20220809
IS  - 1880-3865 (Electronic)
IS  - 1349-0079 (Print)
IS  - 1349-0079 (Linking)
VI  - 64
IP  - 2
DP  - 2022 Jun
TI  - Periodontitis promotes the expression of gingival transmembrane serine protease 2 
      (TMPRSS2), a priming protease for severe acute respiratory syndrome coronavirus 2 
      (SARS-CoV-2).
PG  - 229-236
LID - S1349-0079(22)00058-5 [pii]
LID - 10.1016/j.job.2022.04.004 [doi]
AB  - OBJECTIVES: The oral cavity is one of the main entry sites for SARS-CoV-2. 
      Gingival keratinocytes express transmembrane serine protease 2 (TMPRSS2), 
      responsible for priming the SARS-CoV-2 spike protein. We investigated whether 
      periodontitis increased the expression of TMPRSS2. METHODS: To investigate gene 
      expression in periodontitis, we analyzed the expression of specific genes from 
      (1) the Gene Expression Omnibus (GEO) dataset of 247 human gingival tissues and 
      (2) an experimentally-induced periodontitis mouse model. Human gingival tissues 
      with or without periodontitis were immunohistochemically stained using an 
      anti-TMPRSS2 antibody. Analysis of the TMPRSS2 promoter was performed using a 
      ChIP-Atlas dataset. TMPRSS2 expression was detected in cultured human 
      keratinocytes using quantitative reverse transcription (qRT)-PCR and Western blot 
      analysis. RESULTS: GEO dataset analysis and an experimentally-induced 
      periodontitis model revealed increased expression of TMPRSS2 in periodontitis 
      gingiva. The keratinocyte cell membrane in periodontitis gingiva was strongly 
      immunohistochemically stained for TMPRSS2. Using ChIP-Atlas and GEO datasets, we 
      screened for transcription factors that bind to the TMPRSS2 promoter region. We 
      found one candidate, estrogen receptor 1 (ESR1), highly expressed in 
      periodontitis gingiva. Analysis of the GEO dataset revealed a correlation between 
      ESR1 and TMPRSS2 expression in gingival tissues. An ESR1 ligand induced TMPRSS2 
      expression in cultured keratinocytes. CONCLUSIONS: Periodontitis increases 
      TMPRSS2 expression in the cell membrane of gingival keratinocytes.
CI  - Copyright (c) 2022 Japanese Association for Oral Biology. Published by Elsevier 
      B.V. All rights reserved.
FAU - Ohnishi, Tomokazu
AU  - Ohnishi T
AD  - Department of Oral Biochemistry, Kagoshima University Graduate School of Medical 
      and Dental Sciences, Japan.
FAU - Nakamura, Toshiaki
AU  - Nakamura T
AD  - Department of Periodontology, Kagoshima University Graduate School of Medical and 
      Dental Sciences, Japan.
FAU - Shima, Kaori
AU  - Shima K
AD  - Department of Oral Pathology, Kagoshima University Graduate School of Medical and 
      Dental Sciences, Japan.
FAU - Noguchi, Kazuyuki
AU  - Noguchi K
AD  - Department of Periodontology, Kagoshima University Graduate School of Medical and 
      Dental Sciences, Japan.
FAU - Chiba, Norika
AU  - Chiba N
AD  - Department of Oral Biochemistry, Kagoshima University Graduate School of Medical 
      and Dental Sciences, Japan.
FAU - Matsuguchi, Tetsuya
AU  - Matsuguchi T
AD  - Department of Oral Biochemistry, Kagoshima University Graduate School of Medical 
      and Dental Sciences, Japan. Electronic address: tmatsugu@dent.kagoshima-u.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220425
PL  - Netherlands
TA  - J Oral Biosci
JT  - Journal of oral biosciences
JID - 101226721
RN  - 0 (Spike Glycoprotein, Coronavirus)
RN  - 0 (spike protein, SARS-CoV-2)
RN  - EC 3.4.- (Peptide Hydrolases)
RN  - EC 3.4.17.23 (Angiotensin-Converting Enzyme 2)
RN  - EC 3.4.21.- (Serine Endopeptidases)
RN  - EC 3.4.21.- (TMPRSS2 protein, human)
SB  - IM
MH  - Angiotensin-Converting Enzyme 2
MH  - Animals
MH  - *COVID-19/genetics
MH  - Gingiva
MH  - Humans
MH  - Mice
MH  - Peptide Hydrolases
MH  - *Periodontitis
MH  - SARS-CoV-2
MH  - Serine Endopeptidases/genetics
MH  - Spike Glycoprotein, Coronavirus
PMC - PMC9035663
OTO - NOTNLM
OT  - COVID-19
OT  - Gingiva
OT  - Keratinocytes
OT  - Periodontitis
OT  - SARS-CoV-2
EDAT- 2022/04/27 06:00
MHDA- 2022/05/25 06:00
PMCR- 2022/04/25
CRDT- 2022/04/26 20:05
PHST- 2022/03/31 00:00 [received]
PHST- 2022/04/15 00:00 [revised]
PHST- 2022/04/19 00:00 [accepted]
PHST- 2022/04/27 06:00 [pubmed]
PHST- 2022/05/25 06:00 [medline]
PHST- 2022/04/26 20:05 [entrez]
PHST- 2022/04/25 00:00 [pmc-release]
AID - S1349-0079(22)00058-5 [pii]
AID - 10.1016/j.job.2022.04.004 [doi]
PST - ppublish
SO  - J Oral Biosci. 2022 Jun;64(2):229-236. doi: 10.1016/j.job.2022.04.004. Epub 2022 
      Apr 25.