PMID- 35472537
OWN - NLM
STAT- MEDLINE
DCOM- 20220530
LR  - 20220531
IS  - 1873-3336 (Electronic)
IS  - 0304-3894 (Linking)
VI  - 435
DP  - 2022 Aug 5
TI  - Impact of environmental microplastics alone and mixed with benzo[a]pyrene on 
      cellular and molecular responses of Mytilus galloprovincialis.
PG  - 128952
LID - S0304-3894(22)00741-5 [pii]
LID - 10.1016/j.jhazmat.2022.128952 [doi]
AB  - The hazard of microplastic (MP) pollution in marine environments is a current 
      concern. However, the effects of environmental microplastics combined with other 
      pollutants are still poorly investigated. Herein, impact of ecologically relevant 
      concentrations of environmental MP alone (50 microg/L) or combined with B[a]P 
      (1 microg/L) was assessed in mussel Mytilus galloprovincialis after a short-term 
      exposure (1 and 3 days) to environmental MP collected from a north-Mediterranean 
      beach. Raman Microspectroscopy (RMS) revealed bioaccumulation in mussel hemolymph 
      of MP, characterized by polyethylene (PE), polyethylene terephthalate (PET), 
      polypropylene (PP), polyethylene vinyl acetate (PEVA) and high-density 
      polyethylene (HDPE), with abundance of MP sized 1.22-0.45 microm. An increase of 
      B[a]P was detected in mussels after 3-day exposure, particularly when mixed with 
      MP. Both contaminants induced cytotoxic and genotoxic effects on hemocytes as 
      determined by lysosomal membrane stability (LMS), micronuclei frequency (FMN), 
      and DNA fragmentation rate by terminal dUTP nick-end labeling (TUNEL). About 
      apoptosis/DNA repair processes, P53 and DNA-ligase increased at 1-day exposure in 
      all conditions, whereas after 3 days increase of bax, Cas-3 and P53 and decrease 
      of Bcl-2 and DNA-ligase were revealed, suggesting a shift towards a cell 
      apoptotic event in exposed mussels. Overall, this study provides new insights on 
      the risk of MP for the marine ecosystem, their ability to accumulate xenobiotics 
      and transfer them to marine biota, with potential adverse repercussion on their 
      health status.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Romdhani, Ilef
AU  - Romdhani I
AD  - Laboratory of Agrobiodiversity and Ecotoxicology LR20AGR02, ISA, University of 
      Sousse, Tunisia and Higher Institute of Biotechnology, ISBM, University of 
      Monastir, Tunisia.
FAU - De Marco, Giuseppe
AU  - De Marco G
AD  - Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, 
      University of Messina, Messina 98166, Italy.
FAU - Cappello, Tiziana
AU  - Cappello T
AD  - Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, 
      University of Messina, Messina 98166, Italy. Electronic address: 
      tcappello@unime.it.
FAU - Ibala, Samira
AU  - Ibala S
AD  - Faculty of Medicine of Sousse, University of Sousse, Tunisia.
FAU - Zitouni, Nesrine
AU  - Zitouni N
AD  - Laboratory of Agrobiodiversity and Ecotoxicology LR20AGR02, ISA, University of 
      Sousse, Tunisia and Higher Institute of Biotechnology, ISBM, University of 
      Monastir, Tunisia.
FAU - Boughattas, Iteb
AU  - Boughattas I
AD  - Laboratory of Agrobiodiversity and Ecotoxicology LR20AGR02, ISA, University of 
      Sousse, Tunisia and Higher Institute of Biotechnology, ISBM, University of 
      Monastir, Tunisia.
FAU - Banni, Mohamed
AU  - Banni M
AD  - Laboratory of Agrobiodiversity and Ecotoxicology LR20AGR02, ISA, University of 
      Sousse, Tunisia and Higher Institute of Biotechnology, ISBM, University of 
      Monastir, Tunisia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220419
PL  - Netherlands
TA  - J Hazard Mater
JT  - Journal of hazardous materials
JID - 9422688
RN  - 0 (Microplastics)
RN  - 0 (Plastics)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 0 (Water Pollutants, Chemical)
RN  - 3417WMA06D (Benzo(a)pyrene)
RN  - 9002-88-4 (Polyethylene)
RN  - 9007-49-2 (DNA)
RN  - EC 6.- (Ligases)
SB  - IM
MH  - Animals
MH  - Benzo(a)pyrene/toxicity
MH  - DNA
MH  - Ecosystem
MH  - Ligases/pharmacology
MH  - Microplastics/toxicity
MH  - *Mytilus
MH  - Plastics/toxicity
MH  - Polyethylene/toxicity
MH  - Tumor Suppressor Protein p53
MH  - *Water Pollutants, Chemical/analysis
OTO - NOTNLM
OT  - B[a]P
OT  - DNA fragmentation rate
OT  - Environmental MP
OT  - Lysosomal membrane stability
OT  - Mussels hemolymph
EDAT- 2022/04/27 06:00
MHDA- 2022/05/31 06:00
CRDT- 2022/04/26 20:08
PHST- 2022/02/08 00:00 [received]
PHST- 2022/04/06 00:00 [revised]
PHST- 2022/04/15 00:00 [accepted]
PHST- 2022/04/27 06:00 [pubmed]
PHST- 2022/05/31 06:00 [medline]
PHST- 2022/04/26 20:08 [entrez]
AID - S0304-3894(22)00741-5 [pii]
AID - 10.1016/j.jhazmat.2022.128952 [doi]
PST - ppublish
SO  - J Hazard Mater. 2022 Aug 5;435:128952. doi: 10.1016/j.jhazmat.2022.128952. Epub 
      2022 Apr 19.