PMID- 35472619
OWN - NLM
STAT- MEDLINE
DCOM- 20220613
LR  - 20220614
IS  - 1879-3169 (Electronic)
IS  - 0378-4274 (Linking)
VI  - 363
DP  - 2022 Jun 15
TI  - The MIR100HG/miR-29a-3p/Tab1 axis modulates TGF-beta1-induced fibrotic changes in 
      type II alveolar epithelial cells BLM-caused lung fibrogenesis in mice.
PG  - 45-54
LID - S0378-4274(22)00086-8 [pii]
LID - 10.1016/j.toxlet.2022.04.003 [doi]
AB  - Transforming growth factor (TGF)-beta1-induced fibrotic changes in alveolar 
      epithelium is a critical event in pulmonary fibrosis. Herein, we recognized that 
      lncRNA mir-100-let-7a-2-mir-125b-1 cluster host gene (MIR100HG) was abnormally 
      upregulated within human idiopathic pulmonary fibrosis (IPF) lung tissue, 
      bleomycin (BLM)-caused pulmonary fibrotic model mice and TGF-beta1-stimulated mice 
      type II alveolar epithelial cells. In vivo, MIR100HG knockdown attenuated 
      BLM-caused lung fibrogenesis in mice; in vitro, MIR100HG knockdown attenuated 
      TGF-beta1-induced fibrotic changes in mice type II alveolar epithelial cells. 
      Through direct binding, MIR100HG knockdown upregulated microRNA-29a-3p 
      (miR-29a-3p) expression; through serving as competing endogenous RNA for 
      miR-29a-3p, MIR100HG knockdown downregulated TGF-beta activated kinase 1/MAP3K7 
      binding protein 1 (Tab1) expression. Finally, under TGF-beta1 stimulation, Tab1 
      knockdown attenuated TGF-beta1-induced fibrotic changes and partially attenuated the 
      effects of miR-29a-3p inhibition. In conclusion, we demonstrated the aberrant 
      upregulation of lncRNA MIR100HG in BLM-caused lung fibrogenesis and 
      TGF-beta1-stimulated MLE 12 cells. The MIR100HG/miR-29a-3p/Tab1 axis could modulate 
      TGF-beta1-induced fibrotic changes in type II alveolar epithelial cells and, thus, 
      might be promising targets for pulmonary fibrosis therapy.
CI  - Copyright (c) 2022. Published by Elsevier B.V.
FAU - Guan, Shuhong
AU  - Guan S
AD  - Department of Respiratory and Critical Care Medicine, The First People's Hospital 
      of Changzhou, Changzhou, Jiangsu 213000, China.
FAU - Liu, Hui
AU  - Liu H
AD  - Department of Respiratory and Critical Care Medicine, the Third Clinical Medicine 
      School of Soochow University, Changzhou, Jiangsu 213000, China.
FAU - Zhou, Jun
AU  - Zhou J
AD  - Department of Respiratory and Critical Care Medicine, The First People's Hospital 
      of Changzhou, Changzhou, Jiangsu 213000, China. Electronic address: 
      zsj67720@czfph.com.
FAU - Zhang, Qiudi
AU  - Zhang Q
AD  - Department of Respiratory and Critical Care Medicine, The First People's Hospital 
      of Changzhou, Changzhou, Jiangsu 213000, China.
FAU - Bi, Hui
AU  - Bi H
AD  - Department of Respiratory and Critical Care Medicine, The First People's Hospital 
      of Changzhou, Changzhou, Jiangsu 213000, China.
LA  - eng
PT  - Journal Article
DEP - 20220423
PL  - Netherlands
TA  - Toxicol Lett
JT  - Toxicology letters
JID - 7709027
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn100 microRNA, mouse)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (Tab1 protein, mouse)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 11056-06-7 (Bleomycin)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/metabolism
MH  - Alveolar Epithelial Cells/metabolism
MH  - Animals
MH  - Bleomycin/toxicity
MH  - Epithelial-Mesenchymal Transition
MH  - Fibrosis
MH  - *Idiopathic Pulmonary Fibrosis/chemically induced/genetics/metabolism
MH  - Lung
MH  - Mice
MH  - *MicroRNAs/metabolism
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - Transforming Growth Factor beta1/metabolism
OTO - NOTNLM
OT  - Pulmonary fibrosis
OT  - Tab1
OT  - Type II alveolar epithelial cell
OT  - lncRNA MIR100HG
OT  - miR-29a-3p
EDAT- 2022/04/27 06:00
MHDA- 2022/06/14 06:00
CRDT- 2022/04/26 20:10
PHST- 2021/09/10 00:00 [received]
PHST- 2022/02/14 00:00 [revised]
PHST- 2022/04/20 00:00 [accepted]
PHST- 2022/04/27 06:00 [pubmed]
PHST- 2022/06/14 06:00 [medline]
PHST- 2022/04/26 20:10 [entrez]
AID - S0378-4274(22)00086-8 [pii]
AID - 10.1016/j.toxlet.2022.04.003 [doi]
PST - ppublish
SO  - Toxicol Lett. 2022 Jun 15;363:45-54. doi: 10.1016/j.toxlet.2022.04.003. Epub 2022 
      Apr 23.