PMID- 35472676
OWN - NLM
STAT- MEDLINE
DCOM- 20220603
LR  - 20220603
IS  - 1532-3072 (Electronic)
IS  - 0040-8166 (Linking)
VI  - 76
DP  - 2022 Jun
TI  - Cyanidin attenuates the high hydrostatic pressure-induced degradation of cellular 
      matrix of nucleus pulposus cell via blocking the Wnt/beta-catenin signaling.
PG  - 101798
LID - S0040-8166(22)00070-2 [pii]
LID - 10.1016/j.tice.2022.101798 [doi]
AB  - PURPOSE: The treatment of intervertebral disc degeneration is limited, cyanidin 
      can protect chondrocytes from degeneration. This research investigated the effect 
      of cyanidin on human nucleus pulposus cells (HNPC) metabolism and its mechanism. 
      METHODS: HNPC were treated with cyanidin, XAV-939 (inhibitor of Wnt/beta-catenin 
      signaling) and SKL2001 (activator of Wnt/beta-catenin signaling), and pressurized at 
      1, 3, and 30 atm. Quantitative real-time PCR and Western blot were used to assess 
      the expressions of matrix metalloproteinase-3 (MMP-3), matrix 
      metalloproteinase-13 (MMP-13), Collagen-II, Aggrecan, Wnt-3a and beta-catenin in 
      treated HNPC. Cell counting kit-8 was used to detect the HNPC cell viability. 
      Radioisotope incorporation method was used to assess the proteoglycan synthesis 
      rate of HNPC. The level of cell matrix was detected by toluidine blue staining. 
      RESULTS: Proper hydrostatic pressure of 3 atm could elevate cell viability, 
      proteoglycan synthesis and the level of cell matrix of HNPC, while high 
      hydrostatic pressure of 30 atm reduced the above effects. Cyanidin and XAV-939 
      reversed the promoting effect of 30 atm pressure on Wnt/beta-catenin signaling 
      pathway and the inhibiting effect on cell viability, proteoglycan synthesis and 
      the level of cell matrix. Subsequently, SKL2001 further reversed the function of 
      cyanidin on HNPC. CONCLUSION: Cyanidin attenuated the high hydrostatic 
      pressure-induced degradation of cellular matrix of HNPC via blocking the 
      Wnt/beta-catenin signaling pathway. Our findings in this research provided a basis 
      for in vitro experiment of cyanidin and a theoretical fundament on this disease.
CI  - Copyright (c) 2022. Published by Elsevier Ltd.
FAU - Xu, Yuan
AU  - Xu Y
AD  - Department of orthopedics, Zhejiang Hospital, China. Electronic address: 
      xyuan_xxu@163.com.
FAU - He, Jian
AU  - He J
AD  - Department of orthopedics, Zhejiang Hospital, China.
FAU - He, Jun
AU  - He J
AD  - Department of orthopedics, Zhejiang Hospital, China.
LA  - eng
PT  - Journal Article
DEP - 20220412
PL  - Scotland
TA  - Tissue Cell
JT  - Tissue & cell
JID - 0214745
RN  - 0 (Aggrecans)
RN  - 0 (Anthocyanins)
RN  - 0 (beta Catenin)
RN  - 7732ZHU564 (cyanidin)
SB  - IM
MH  - Aggrecans
MH  - Anthocyanins
MH  - Cells, Cultured
MH  - Humans
MH  - Hydrostatic Pressure
MH  - *Intervertebral Disc Degeneration/drug therapy
MH  - *Nucleus Pulposus/metabolism
MH  - Wnt Signaling Pathway
MH  - beta Catenin/metabolism
OTO - NOTNLM
OT  - Cyanidin
OT  - HNPC
OT  - Hydrostatic pressure
OT  - Intervertebral disc degeneration
OT  - Wnt/beta-catenin signaling pathway
EDAT- 2022/04/27 06:00
MHDA- 2022/06/07 06:00
CRDT- 2022/04/26 20:14
PHST- 2022/02/11 00:00 [received]
PHST- 2022/03/29 00:00 [revised]
PHST- 2022/04/08 00:00 [accepted]
PHST- 2022/04/27 06:00 [pubmed]
PHST- 2022/06/07 06:00 [medline]
PHST- 2022/04/26 20:14 [entrez]
AID - S0040-8166(22)00070-2 [pii]
AID - 10.1016/j.tice.2022.101798 [doi]
PST - ppublish
SO  - Tissue Cell. 2022 Jun;76:101798. doi: 10.1016/j.tice.2022.101798. Epub 2022 Apr 
      12.