PMID- 35474221
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 2057-3995 (Electronic)
IS  - 2057-3995 (Linking)
VI  - 7
IP  - 1
DP  - 2022 Apr 26
TI  - Biomechanically and biochemically functional scaffold for recruitment of 
      endogenous stem cells to promote tendon regeneration.
PG  - 26
LID - 10.1038/s41536-022-00220-z [doi]
LID - 26
AB  - Tendon regeneration highly relies on biomechanical and biochemical cues in the 
      repair microenvironment. Herein, we combined the decellularized bovine tendon 
      sheet (DBTS) with extracellular matrix (ECM) from tendon-derived stem cells 
      (TDSCs) to fabricate a biomechanically and biochemically functional scaffold 
      (tECM-DBTS), to provide a functional and stem cell ECM-based microenvironment for 
      tendon regeneration. Our prior study showed that DBTS was biomechanically 
      suitable to tendon repair. In this study, the biological function of tECM-DBTS 
      was examined in vitro, and the efficiency of the scaffold for Achilles tendon 
      repair was evaluated using immunofluorescence staining, histological staining, 
      stem cell tracking, biomechanical and functional analyses. It was found that 
      tECM-DBTS increased the content of bioactive factors and had a better performance 
      for the proliferation, migration and tenogenic differentiation of bone 
      marrow-derived stem cells (BMSCs) than DBTS. Furthermore, our results 
      demonstrated that tECM-DBTS promoted tendon regeneration and improved the 
      biomechanical properties of regenerated Achilles tendons in rats by recruiting 
      endogenous stem cells and participating in the functionalization of these stem 
      cells. As a whole, the results of this study demonstrated that the tECM-DBTS can 
      provide a bionic microenvironment for recruiting endogenous stem cells and 
      facilitating in situ regeneration of tendons.
CI  - (c) 2022. The Author(s).
FAU - Cui, Jing
AU  - Cui J
AUID- ORCID: 0000-0003-3899-4613
AD  - Laboratory of Stem Cell and Tissue Engineering, Orthopedic Research Institute, 
      State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, 
      Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, 
      China.
FAU - Ning, Liang-Ju
AU  - Ning LJ
AD  - Laboratory of Stem Cell and Tissue Engineering, Orthopedic Research Institute, 
      State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, 
      Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, 
      China.
FAU - Wu, Fei-Peng
AU  - Wu FP
AD  - Department of Orthopedic Surgery, West China Hospital, Sichuan University, 
      Chengdu, China.
AD  - Panzhihua Central Hospital, Panzhihua, China.
FAU - Hu, Ruo-Nan
AU  - Hu RN
AD  - Laboratory of Stem Cell and Tissue Engineering, Orthopedic Research Institute, 
      State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, 
      Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, 
      China.
FAU - Li, Xuan
AU  - Li X
AD  - Laboratory of Stem Cell and Tissue Engineering, Orthopedic Research Institute, 
      State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, 
      Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, 
      China.
FAU - He, Shu-Kun
AU  - He SK
AD  - Department of Orthopedic Surgery, West China Hospital, Sichuan University, 
      Chengdu, China.
FAU - Zhang, Yan-Jing
AU  - Zhang YJ
AD  - Laboratory of Stem Cell and Tissue Engineering, Orthopedic Research Institute, 
      State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, 
      Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, 
      China.
FAU - Luo, Jia-Jiao
AU  - Luo JJ
AD  - Laboratory of Stem Cell and Tissue Engineering, Orthopedic Research Institute, 
      State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, 
      Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, 
      China.
FAU - Luo, Jing-Cong
AU  - Luo JC
AD  - Laboratory of Stem Cell and Tissue Engineering, Orthopedic Research Institute, 
      State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, 
      Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, 
      China.
FAU - Qin, Ting-Wu
AU  - Qin TW
AUID- ORCID: 0000-0003-1063-857X
AD  - Laboratory of Stem Cell and Tissue Engineering, Orthopedic Research Institute, 
      State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, 
      Sichuan University and Collaborative Innovation Center of Biotherapy, Chengdu, 
      China. tingwuqin@hotmail.com.
LA  - eng
GR  - 32071348/National Natural Science Foundation of China (National Science 
      Foundation of China)/
PT  - Journal Article
DEP - 20220426
PL  - United States
TA  - NPJ Regen Med
JT  - NPJ Regenerative medicine
JID - 101699846
PMC - PMC9043181
COIS- The authors declare no competing interests.
EDAT- 2022/04/28 06:00
MHDA- 2022/04/28 06:01
PMCR- 2022/04/26
CRDT- 2022/04/27 05:45
PHST- 2021/07/29 00:00 [received]
PHST- 2022/03/22 00:00 [accepted]
PHST- 2022/04/27 05:45 [entrez]
PHST- 2022/04/28 06:00 [pubmed]
PHST- 2022/04/28 06:01 [medline]
PHST- 2022/04/26 00:00 [pmc-release]
AID - 10.1038/s41536-022-00220-z [pii]
AID - 220 [pii]
AID - 10.1038/s41536-022-00220-z [doi]
PST - epublish
SO  - NPJ Regen Med. 2022 Apr 26;7(1):26. doi: 10.1038/s41536-022-00220-z.