PMID- 35474406 OWN - NLM STAT- MEDLINE DCOM- 20220809 LR - 20220810 IS - 1438-7948 (Electronic) IS - 1438-793X (Linking) VI - 22 IP - 4 DP - 2022 Aug TI - Knockdown of circMYOF inhibits cell growth, metastasis, and glycolysis through miR-145-5p/OTX1 regulatory axis in laryngeal squamous cell carcinoma. PG - 1-13 LID - 10.1007/s10142-022-00862-8 [doi] AB - New evidence suggests that abnormal expression of circular RNA (circRNA) is associated with the development of human cancers. This study aims to reveal circMYOF roles in the malignant phenotype of laryngeal squamous cell carcinoma (LSCC). The expression of circMYOF, microRNA (miR)-145-5p, and orthodenticle homeobox 1 (OTX1) was detected by quantitative real-time PCR. Cell proliferation, migration, invasion, and apoptosis were determined using colony formation assay and EdU assay, wound healing assay, transwell assay, and flow cytometry, respectively. Protein expression was examined by western blot analysis. Cell glycolysis was assessed by detecting glucose consumption and lactate production. Mice xenograft models were constructed to evaluate the regulation of circMYOF on LSCC tumorigenesis. The regulatory relationships among circMYOF, miR-145-5p, and OTX1 were identified using dual-luciferase reporter assay and RIP assay. Serum exosomes were isolated to confirm the existence of circMYOF in LSCC patients. CircMYOF was upregulated in LSCC tissues and cells, and its knockdown suppressed LSCC cell growth, metastasis, and glycolysis, as well as inhibited LSCC tumor growth. MiR-145-5p had decreased expression in LSCC, and it could be sponged by circMYOF. The inhibition effect of circMYOF lentivirus short hairpin RNA (sh-circMYOF) on LSCC progression was restored by the inhibitor of miR-145-5p (in-miR-145-5p). Also, OTX1 was targeted by miR-145-5p and was positively regulated by circMYOF. MiR-145-5p could repress LSCC progression, and OTX1 overexpression also eliminated this effect. In addition, we found that circMYOF was significantly overexpressed in the serum exosomes of LSCC patients. Our data revealed that circMYOF contributed to LSCC progression by promoting cell growth, metastasis, and glycolysis through miR-145-5p/OTX1 axis. CI - (c) 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. FAU - Li, Shihua AU - Li S AD - Department of ENT & HN Surgery, Stomatological Hospital affiliated to Kunming Medical University, Yunnan Province, Kunming, 650032, People's Republic of China. AD - Department of Acupuncture and Massage, Yuxi People's Hospital, Yunnan Province, Yuxi, 653199, People's Republic of China. FAU - Zhang, Ying AU - Zhang Y AD - Department of Acupuncture and Massage, Yuxi People's Hospital, Yunnan Province, Yuxi, 653199, People's Republic of China. FAU - He, Zhongshun AU - He Z AD - Department of ENT & HN Surgery, Stomatological Hospital affiliated to Kunming Medical University, Yunnan Province, Kunming, 650032, People's Republic of China. FAU - Xu, Qiannan AU - Xu Q AD - Department of Acupuncture and Massage, Yuxi People's Hospital, Yunnan Province, Yuxi, 653199, People's Republic of China. FAU - Li, Cailian AU - Li C AD - Department of Acupuncture and Massage, Yuxi People's Hospital, Yunnan Province, Yuxi, 653199, People's Republic of China. FAU - Xu, Biao AU - Xu B AUID- ORCID: 0000-0003-3625-841X AD - Department of Oral and Maxillofacial Surgery, Yuxi People's Hospital, Yunnan Province, 653100, Yuxi, People's Republic of China. djjian2020@163.com. AD - , Kunming City, People's Republic of China. djjian2020@163.com. LA - eng PT - Journal Article DEP - 20220426 PL - Germany TA - Funct Integr Genomics JT - Functional & integrative genomics JID - 100939343 RN - 0 (MIRN145 microRNA, human) RN - 0 (MicroRNAs) RN - 0 (OTX1 protein, human) RN - 0 (Otx Transcription Factors) RN - 0 (RNA, Circular) SB - IM MH - Animals MH - Cell Line, Tumor MH - Cell Proliferation/genetics MH - Gene Expression Regulation, Neoplastic MH - Glycolysis/genetics MH - Humans MH - *Laryngeal Neoplasms/genetics MH - Mice MH - *MicroRNAs/genetics MH - *Otx Transcription Factors/genetics MH - *RNA, Circular/genetics MH - *Squamous Cell Carcinoma of Head and Neck/genetics OTO - NOTNLM OT - Laryngeal squamous cell carcinoma OT - OTX1 OT - circMYOF OT - miR-145-5p EDAT- 2022/04/28 06:00 MHDA- 2022/08/10 06:00 CRDT- 2022/04/27 05:57 PHST- 2021/12/30 00:00 [received] PHST- 2022/04/11 00:00 [accepted] PHST- 2022/04/11 00:00 [revised] PHST- 2022/04/28 06:00 [pubmed] PHST- 2022/08/10 06:00 [medline] PHST- 2022/04/27 05:57 [entrez] AID - 10.1007/s10142-022-00862-8 [pii] AID - 10.1007/s10142-022-00862-8 [doi] PST - ppublish SO - Funct Integr Genomics. 2022 Aug;22(4):1-13. doi: 10.1007/s10142-022-00862-8. Epub 2022 Apr 26.