PMID- 35474604
OWN - NLM
STAT- MEDLINE
DCOM- 20220603
LR  - 20230916
IS  - 1759-7714 (Electronic)
IS  - 1759-7706 (Print)
IS  - 1759-7706 (Linking)
VI  - 13
IP  - 11
DP  - 2022 Jun
TI  - Activation of circ_0072088/miR-1261/PIK3CA pathway accelerates lung 
      adenocarcinoma progression.
PG  - 1548-1557
LID - 10.1111/1759-7714.14369 [doi]
AB  - BACKGROUND: Circular RNAs (circRNAs) are involved in the tumorigenesis and 
      progression of lung adenocarcinoma (LUAD). This study aimed to determine the role 
      of circ_0072088 in LUAD. METHODS: The existence and expression of circ_0072088 in 
      human LUAD tissues and cell lines were determined through Sanger sequencing, 
      quantitative reverse transcription-polymerase chain reaction, and fluorescence in 
      situ hybridization (FISH). Subsequently, the biological role of circ_0072088 was 
      examined using loss-of-function assays in H1299 cells. Moreover, 
      circ_0072088/miR-1261/PIK3CA pathway-mediated biological effects in H1299 were 
      verified using bioinformatic prediction and experiments, including interaction 
      analysis (FISH, luciferase reporter, and RNA-pulldown assays), and tumor 
      biological function test (CCK8 and colony formation, wound healing, and transwell 
      assays). Finally, miR-1261 and PIK3CA expression and LUAD patient survival were 
      further analyzed using FISH, immunohistochemical staining, and the Kaplan-Meier 
      plotter database, respectively. RESULTS: First, an increase in circ_0072088 was 
      confirmed in human LUAD tissues. Thereafter, it was mainly localized in the 
      cytoplasm and was found to enhance cell proliferation, migration, and invasion of 
      H1299 cells. Mechanistically, circ_0072088 directly downregulated miR-1261 
      expression, whereas increased PIK3CA gene expression was associated with poor 
      overall survival of LUAD patients. The activation of the 
      circ_0072088/miR-1261/PIK3CA regulatory pathway may play a significant role in 
      the tumorigenesis and progression of LUAD. CONCLUSIONS: Circ_0072088-dependent 
      regulation of miR-1261/PIK3CA is important for cell proliferation, migration, and 
      invasion during the tumorigenesis and progression of LUAD, warranting the need to 
      consider the circ_0072088/miR-1261/PIK3CA regulatory pathway as a potential 
      therapeutic target in patients with lung adenocarcinoma.
CI  - (c) 2022 The Authors. Thoracic Cancer published by China Lung Oncology Group and 
      John Wiley & Sons Australia, Ltd.
FAU - Cao, Feng
AU  - Cao F
AUID- ORCID: 0000-0003-1604-1723
AD  - Department of Radiation Oncology, the Fourth Hospital of HebeiMedical University, 
      Shijiazhuang, China.
FAU - Liu, Sihua
AU  - Liu S
AD  - Research Center, the Fourth Hospital of HebeiMedical University, Shijiazhuang, 
      China.
FAU - Li, Ziyi
AU  - Li Z
AD  - Research Center, the Fourth Hospital of HebeiMedical University, Shijiazhuang, 
      China.
FAU - Meng, Lingjiao
AU  - Meng L
AD  - Research Center, the Fourth Hospital of HebeiMedical University, Shijiazhuang, 
      China.
FAU - Sang, Meixiang
AU  - Sang M
AD  - Research Center, the Fourth Hospital of HebeiMedical University, Shijiazhuang, 
      China.
FAU - Shan, Baoen
AU  - Shan B
AUID- ORCID: 0000-0003-4775-104X
AD  - Research Center, the Fourth Hospital of HebeiMedical University, Shijiazhuang, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220426
PL  - Singapore
TA  - Thorac Cancer
JT  - Thoracic cancer
JID - 101531441
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
RN  - EC 2.7.1.137 (Class I Phosphatidylinositol 3-Kinases)
RN  - EC 2.7.1.137 (PIK3CA protein, human)
SB  - IM
MH  - *Adenocarcinoma of Lung/genetics/metabolism/pathology
MH  - Carcinogenesis
MH  - Cell Line, Tumor
MH  - Cell Proliferation/physiology
MH  - *Class I Phosphatidylinositol 3-Kinases/genetics
MH  - Disease Progression
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Lung Neoplasms/genetics/metabolism/pathology
MH  - *MicroRNAs/genetics/metabolism
MH  - *RNA, Circular/genetics/metabolism
MH  - Signal Transduction
PMC - PMC9161339
OTO - NOTNLM
OT  - bioinformatics
OT  - cell lines
OT  - circRNAs
OT  - lung adenocarcinoma
OT  - tumorigenesis
COIS- The authors declare no conflict of interest, financial or otherwise.
EDAT- 2022/04/28 06:00
MHDA- 2022/06/07 06:00
PMCR- 2022/06/01
CRDT- 2022/04/27 06:09
PHST- 2022/02/13 00:00 [revised]
PHST- 2022/01/18 00:00 [received]
PHST- 2022/02/14 00:00 [accepted]
PHST- 2022/04/28 06:00 [pubmed]
PHST- 2022/06/07 06:00 [medline]
PHST- 2022/04/27 06:09 [entrez]
PHST- 2022/06/01 00:00 [pmc-release]
AID - TCA14369 [pii]
AID - 10.1111/1759-7714.14369 [doi]
PST - ppublish
SO  - Thorac Cancer. 2022 Jun;13(11):1548-1557. doi: 10.1111/1759-7714.14369. Epub 2022 
      Apr 26.