PMID- 35474964
OWN - NLM
STAT- MEDLINE
DCOM- 20231002
LR  - 20231002
IS  - 2667-2375 (Electronic)
IS  - 2667-2375 (Linking)
VI  - 2
IP  - 2
DP  - 2022 Feb 28
TI  - A Flp-dependent G-CaMP9a transgenic mouse for neuronal imaging in vivo.
PG  - 100168
LID - 10.1016/j.crmeth.2022.100168 [doi]
LID - 100168
AB  - Genetically encoded calcium indicators (GECIs) are widely used to measure calcium 
      transients in neuronal somata and processes, and their use enables the 
      determination of action potential temporal series in a large population of 
      neurons. Here, we generate a transgenic mouse line expressing a highly sensitive 
      green GECI, G-CaMP9a, in a Flp-dependent manner in excitatory and inhibitory 
      neuronal subpopulations downstream of a strong CAG promoter. Combining this 
      reporter mouse with viral or mouse genetic Flp delivery methods produces a robust 
      and stable G-CaMP9a expression in defined neuronal populations without detectable 
      detrimental effects. In vivo two-photon imaging reveals spontaneous and 
      sensory-evoked calcium transients in excitatory and inhibitory ensembles with 
      cellular resolution. Our results show that this reporter line allows long-term, 
      cell-type-specific investigation of neuronal activity with enhanced resolution in 
      defined populations and facilitates dissecting complex dynamics of neural 
      networks in vivo.
CI  - (c) 2022 The Author(s).
FAU - Sakamoto, Masayuki
AU  - Sakamoto M
AD  - Department of Neurochemistry, Graduate School of Medicine, The University of 
      Tokyo, Hongo7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan.
AD  - Department of Optical Neural and Molecular Physiology, Graduate School of 
      Biostudies, Kyoto University, Kyoto 606-8507, Japan.
AD  - Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science 
      and Technology Agency, Kyoto 606-8507, Japan.
FAU - Inoue, Masatoshi
AU  - Inoue M
AD  - Department of Neurochemistry, Graduate School of Medicine, The University of 
      Tokyo, Hongo7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan.
AD  - Department of Bioengineering, Stanford University, Stanford, CA 94305, USA.
FAU - Takeuchi, Atsuya
AU  - Takeuchi A
AD  - Department of Neurophysiology, Graduate School of Medicine, The University of 
      Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
AD  - Department of Neurophysiology, School of Dentistry, Tokyo Medical and Dental 
      University, Tokyo, Japan.
FAU - Kobari, Shigetaka
AU  - Kobari S
AD  - Department of Neurochemistry, Graduate School of Medicine, The University of 
      Tokyo, Hongo7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan.
FAU - Yokoyama, Tatsushi
AU  - Yokoyama T
AD  - Department of Neurochemistry, Graduate School of Medicine, The University of 
      Tokyo, Hongo7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan.
AD  - Department of Optical Neural and Molecular Physiology, Graduate School of 
      Biostudies, Kyoto University, Kyoto 606-8507, Japan.
FAU - Horigane, Shin-Ichiro
AU  - Horigane SI
AD  - Department of Neurochemistry, Graduate School of Medicine, The University of 
      Tokyo, Hongo7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan.
AD  - Department of Neuroscience I, Research Institute of Environmental Medicine, 
      Nagoya University, Nagoya, Aichi 464-8602, Japan.
AD  - Department of Molecular/Cellular Neuroscience, Nagoya University Graduate School 
      of Medicine, Nagoya, Aichi 466-8550, Japan.
FAU - Takemoto-Kimura, Sayaka
AU  - Takemoto-Kimura S
AD  - Department of Neurochemistry, Graduate School of Medicine, The University of 
      Tokyo, Hongo7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan.
AD  - Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science 
      and Technology Agency, Kyoto 606-8507, Japan.
AD  - Department of Neuroscience I, Research Institute of Environmental Medicine, 
      Nagoya University, Nagoya, Aichi 464-8602, Japan.
AD  - Department of Molecular/Cellular Neuroscience, Nagoya University Graduate School 
      of Medicine, Nagoya, Aichi 466-8550, Japan.
FAU - Abe, Manabu
AU  - Abe M
AD  - Department of Animal Model Development, Brain Research Institute, Niigata 
      University, Niigata 951-8585, Japan.
FAU - Sakimura, Kenji
AU  - Sakimura K
AD  - Department of Animal Model Development, Brain Research Institute, Niigata 
      University, Niigata 951-8585, Japan.
FAU - Kano, Masanobu
AU  - Kano M
AD  - Department of Neurophysiology, Graduate School of Medicine, The University of 
      Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
AD  - International Research Center for Neurointelligence (WPI-IRCN), The University of 
      Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
FAU - Kitamura, Kazuo
AU  - Kitamura K
AD  - Department of Neurophysiology, Graduate School of Medicine, The University of 
      Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
AD  - Department of Neurophysiology, Faculty of Medicine, University of Yamanashi, 
      Chuo, Yamanashi 409-3898, Japan.
FAU - Fujii, Hajime
AU  - Fujii H
AD  - Department of Neurochemistry, Graduate School of Medicine, The University of 
      Tokyo, Hongo7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan.
FAU - Bito, Haruhiko
AU  - Bito H
AD  - Department of Neurochemistry, Graduate School of Medicine, The University of 
      Tokyo, Hongo7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan.
AD  - International Research Center for Neurointelligence (WPI-IRCN), The University of 
      Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220214
PL  - United States
TA  - Cell Rep Methods
JT  - Cell reports methods
JID - 9918227360606676
RN  - SY7Q814VUP (Calcium)
MH  - Animals
MH  - Mice
MH  - Action Potentials
MH  - *Calcium/metabolism
MH  - Mice, Transgenic
MH  - *Neurons
MH  - *Neuroimaging
PMC - PMC9017135
OTO - NOTNLM
OT  - Flp/Frt recombination
OT  - dual-color imaging
OT  - genetically encoded calcium indicator
OT  - in vivo calcium imaging
OT  - transgenic mouse
OT  - two-photon microscopy
COIS- The authors declare no competing interests.
EDAT- 2022/04/28 06:00
MHDA- 2022/04/28 06:01
PMCR- 2022/02/14
CRDT- 2022/04/27 06:18
PHST- 2021/09/02 00:00 [received]
PHST- 2021/12/09 00:00 [revised]
PHST- 2022/01/21 00:00 [accepted]
PHST- 2022/04/27 06:18 [entrez]
PHST- 2022/04/28 06:00 [pubmed]
PHST- 2022/04/28 06:01 [medline]
PHST- 2022/02/14 00:00 [pmc-release]
AID - S2667-2375(22)00020-0 [pii]
AID - 100168 [pii]
AID - 10.1016/j.crmeth.2022.100168 [doi]
PST - epublish
SO  - Cell Rep Methods. 2022 Feb 14;2(2):100168. doi: 10.1016/j.crmeth.2022.100168. 
      eCollection 2022 Feb 28.