PMID- 35478034
OWN - NLM
STAT- MEDLINE
DCOM- 20220524
LR  - 20220524
IS  - 1618-2650 (Electronic)
IS  - 1618-2642 (Linking)
VI  - 414
IP  - 14
DP  - 2022 Jun
TI  - A proteomics strategy for the identification of multiple sites in sulfur 
      mustard-modified HSA and screening potential biomarkers for retrospective 
      analysis of exposed human plasma.
PG  - 4179-4188
LID - 10.1007/s00216-022-04070-y [doi]
AB  - A major challenge for the unequivocal verification of alleged exposure to sulfur 
      mustard (HD) lies in identifying its multiple modifications on endogenous 
      proteins and utilizing these modified proteins to achieve accurate, sensitive, 
      and rapid detection for retrospective analysis of HD exposure. As the most 
      abundant protein in human plasma, human serum albumin (HSA) can react with many 
      xenobiotics, such as HD, to protect the body from damage. The HSA adducts induced 
      by HD have been used as biomarkers for the verification of HD exposure. In this 
      study, the modification sites on HSA by HD were identified through application of 
      the bottom-up strategy used in proteomics, and 41 modified sites were discovered 
      with seven types of amino acids, of which 3 types were not previously reported. 
      Then, different enzymes, including pepsin, endoproteinase Glu-C, and pronase, 
      were applied to digest HD-HSA to produce adducts with hydroxyethylthioethyl 
      (HETE) groups, which may be used as potential biomarkers for HD exposure. As 
      candidates for retrospective analysis, sixteen adducts were obtained and 
      characterized with ultra-high-pressure liquid chromatography coupled with 
      quadrupole-Orbitrap mass spectrometry (UHPLC-QE Focus MS). These potential 
      biomarkers were evaluated in human plasma that was exposed in vitro to HD and 
      five of its analogues. This study integrated the identification of modification 
      sites through application of the bottom-up strategy of proteomics and screening 
      biomarkers, providing a novel strategy for retrospective detection of the 
      exposure of xenobiotic chemicals.
CI  - (c) 2022. Springer-Verlag GmbH Germany, part of Springer Nature.
FAU - Chen, Bo
AU  - Chen B
AD  - State Key Laboratory of NBC Protection for Civilian, Laboratory of Analytical 
      Chemistry, Research Institute of Chemical Defence, Beijing, 102205, People's 
      Republic of China.
FAU - Zhang, Qiaoli
AU  - Zhang Q
AD  - State Key Laboratory of NBC Protection for Civilian, Laboratory of Analytical 
      Chemistry, Research Institute of Chemical Defence, Beijing, 102205, People's 
      Republic of China.
FAU - Ren, Zhe
AU  - Ren Z
AD  - School of Chemistry and Chemical Engineering, Nanjing University of Sciences & 
      Technology, Nanjing, 210094, People's Republic of China.
FAU - Zhang, Tao
AU  - Zhang T
AD  - State Key Laboratory of Proteomics, Beijing Proteome Research Center, National 
      Center for Protein Sciences (Beijing), Research Unit of Proteomics & Research, 
      Development of New Drug of Chinese Academy of Medical Sciences, Institute of 
      Lifeomics, Beijing, 102206, People's Republic of China.
FAU - Yu, Huilan
AU  - Yu H
AD  - State Key Laboratory of NBC Protection for Civilian, Laboratory of Analytical 
      Chemistry, Research Institute of Chemical Defence, Beijing, 102205, People's 
      Republic of China.
FAU - Liu, Changcai
AU  - Liu C
AD  - State Key Laboratory of NBC Protection for Civilian, Laboratory of Analytical 
      Chemistry, Research Institute of Chemical Defence, Beijing, 102205, People's 
      Republic of China.
FAU - Yang, Yang
AU  - Yang Y
AD  - State Key Laboratory of NBC Protection for Civilian, Laboratory of Analytical 
      Chemistry, Research Institute of Chemical Defence, Beijing, 102205, People's 
      Republic of China.
FAU - Xu, Ping
AU  - Xu P
AD  - State Key Laboratory of Proteomics, Beijing Proteome Research Center, National 
      Center for Protein Sciences (Beijing), Research Unit of Proteomics & Research, 
      Development of New Drug of Chinese Academy of Medical Sciences, Institute of 
      Lifeomics, Beijing, 102206, People's Republic of China. xuping_bprc@126.com.
FAU - Liu, Shilei
AU  - Liu S
AD  - State Key Laboratory of NBC Protection for Civilian, Laboratory of Analytical 
      Chemistry, Research Institute of Chemical Defence, Beijing, 102205, People's 
      Republic of China. liu_shilei@lacricd.com.
LA  - eng
PT  - Journal Article
DEP - 20220427
PL  - Germany
TA  - Anal Bioanal Chem
JT  - Analytical and bioanalytical chemistry
JID - 101134327
RN  - 0 (Biomarkers)
RN  - 0 (Chemical Warfare Agents)
RN  - T8KEC9FH9P (Mustard Gas)
RN  - ZIF514RVZR (Serum Albumin, Human)
SB  - IM
MH  - Biomarkers/analysis
MH  - *Chemical Warfare Agents/analysis
MH  - Humans
MH  - *Mustard Gas/analysis
MH  - Proteomics
MH  - Retrospective Studies
MH  - Serum Albumin, Human/chemistry
MH  - Tandem Mass Spectrometry/methods
OTO - NOTNLM
OT  - Exposure biomarkers
OT  - Human serum albumin
OT  - Proteomics
OT  - Sulfur mustard
EDAT- 2022/04/29 06:00
MHDA- 2022/05/25 06:00
CRDT- 2022/04/28 05:40
PHST- 2022/03/02 00:00 [received]
PHST- 2022/04/06 00:00 [accepted]
PHST- 2022/03/27 00:00 [revised]
PHST- 2022/04/29 06:00 [pubmed]
PHST- 2022/05/25 06:00 [medline]
PHST- 2022/04/28 05:40 [entrez]
AID - 10.1007/s00216-022-04070-y [pii]
AID - 10.1007/s00216-022-04070-y [doi]
PST - ppublish
SO  - Anal Bioanal Chem. 2022 Jun;414(14):4179-4188. doi: 10.1007/s00216-022-04070-y. 
      Epub 2022 Apr 27.