PMID- 35479581
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220716
IS  - 1687-966X (Print)
IS  - 1687-9678 (Electronic)
VI  - 2022
DP  - 2022
TI  - High-Throughput Sequencing Reveals CXCR4 and IGF1 Behave Different Roles in 
      Weightlessness Osteoporosis.
PG  - 5719077
LID - 10.1155/2022/5719077 [doi]
LID - 5719077
AB  - OBJECTIVE: This study is aimed at screening the differential expression profiles 
      of mRNA under weightlessness osteoporosis through high-throughput sequencing 
      technology, as well as investigating the pathogenesis of weightlessness 
      osteoporosis at the molecular level especially in bone marrow mesenchymal stem 
      cells (BMSCs). METHODS: The mouse bone marrow mesenchymal stem cell line was 
      divided into ground group and simulated microgravity (SMG) group. BMP-2 was used 
      to induce osteogenic differentiation, and SMG group was placed into 2D-gyroscope 
      to simulate weightless condition. Transcriptome sequencing was performed by 
      Illumina technology, DEGs between ground and SMG group was conducted using the 
      DEseq2 algorithm. Molecular functions and signaling pathways enriched by DEGs 
      were then comprehensively analyzed via multiple bioinformatic approaches 
      including but not limited to GO, KEGG, GSEA, and PPI analysis. RESULTS: A total 
      of 263 DEGs were identified by comparing these 2 groups, including 186 
      upregulated genes and 77 downregulated genes. GO analysis showed that DEGs were 
      enriched in osteoblasts, osteoclasts cell proliferation, differentiation, and 
      apoptosis; KEGG analysis revealed that DEGs were significantly enriched in the 
      TNF signaling pathway and FoxO signaling pathway; the enrichment results from 
      Reactome database displayed that DEGs were mainly involved in the transcription 
      of Hoxb3 gene, RUNX1 recruitment KMT2A gene, and activation of Hoxa2 chromatin 
      signaling pathway. The four genes, IL6, CXCR4, IGF1, and PLOD2, were identified 
      as hub genes for subsequent analysis. CONCLUSIONS: This study elucidated the 
      significance of 10 hub genes in the development of weightlessness osteoporosis. 
      In addition, the results of this study provide a theoretical basis and novel 
      ideas for the subsequent research of the pathogenesis and clinical treatment of 
      weightlessness osteoporosis.
CI  - Copyright (c) 2022 Dong Wang et al.
FAU - Wang, Dong
AU  - Wang D
AD  - Department of Orthopedic Surgery, Xijing Hospital, Air Force Medical University, 
      Xi'an, China.
AD  - Department of Orthopaedics, Affiliated Hospital of Yanan University, Yanan 
      716000, China.
FAU - Li, Weihang
AU  - Li W
AD  - Department of Orthopedic Surgery, Xijing Hospital, Air Force Medical University, 
      Xi'an, China.
FAU - Ding, Ziyi
AU  - Ding Z
AD  - Department of Orthopedic Surgery, Xijing Hospital, Air Force Medical University, 
      Xi'an, China.
FAU - Shi, Quan
AU  - Shi Q
AD  - Department of Orthopedic Surgery, Xijing Hospital, Air Force Medical University, 
      Xi'an, China.
AD  - Department of Orthopaedics, Affiliated Hospital of Yanan University, Yanan 
      716000, China.
FAU - Zhang, Shilei
AU  - Zhang S
AD  - Department of Orthopedic Surgery, Xijing Hospital, Air Force Medical University, 
      Xi'an, China.
FAU - Zhang, Zhuoru
AU  - Zhang Z
AD  - Center of Clinical Aerospace Medicine, School of Aerospace Medicine, Air Force 
      Medical University, Xi'an 710032, China.
FAU - Liu, Zhibin
AU  - Liu Z
AUID- ORCID: 0000-0001-8017-0963
AD  - Department of Orthopaedics, Affiliated Hospital of Yanan University, Yanan 
      716000, China.
FAU - Wang, Xiaocheng
AU  - Wang X
AUID- ORCID: 0000-0002-2220-6398
AD  - Center of Clinical Aerospace Medicine, School of Aerospace Medicine, Air Force 
      Medical University, Xi'an 710032, China.
AD  - Department of Aviation Medicine, The First Affiliated Hospital, Air Force Medical 
      University, Xi'an 710032, China.
FAU - Yan, Ming
AU  - Yan M
AUID- ORCID: 0000-0003-0669-1087
AD  - Department of Orthopedic Surgery, Xijing Hospital, Air Force Medical University, 
      Xi'an, China.
LA  - eng
PT  - Journal Article
DEP - 20220418
PL  - United States
TA  - Stem Cells Int
JT  - Stem cells international
JID - 101535822
PMC - PMC9038434
COIS- All authors declared no conflicts of interests related to this manuscript, and 
      all authors have approved the publication of this work.
EDAT- 2022/04/29 06:00
MHDA- 2022/04/29 06:01
PMCR- 2022/04/18
CRDT- 2022/04/28 06:20
PHST- 2022/03/03 00:00 [received]
PHST- 2022/03/24 00:00 [accepted]
PHST- 2022/04/28 06:20 [entrez]
PHST- 2022/04/29 06:00 [pubmed]
PHST- 2022/04/29 06:01 [medline]
PHST- 2022/04/18 00:00 [pmc-release]
AID - 10.1155/2022/5719077 [doi]
PST - epublish
SO  - Stem Cells Int. 2022 Apr 18;2022:5719077. doi: 10.1155/2022/5719077. eCollection 
      2022.