PMID- 35483786
OWN - NLM
STAT- MEDLINE
DCOM- 20220502
LR  - 20220502
IS  - 1879-3592 (Electronic)
IS  - 1383-5718 (Linking)
VI  - 876-877
DP  - 2022 Apr-May
TI  - Pyrene-polyethylene glycol-modified multi-walled carbon nanotubes: Genotoxicity 
      in V79-4 fibroblast cells.
PG  - 503463
LID - S1383-5718(22)00024-9 [pii]
LID - 10.1016/j.mrgentox.2022.503463 [doi]
AB  - The genotoxicity of pyrene-polyethylene glycol-modified multi-walled carbon 
      nanotubes (MWCNT-PyPEG), engineered as a nanoplatform for bioapplication, was 
      evaluated. Toxicity was assessed in hamster lung fibroblast cells (V79-4). MTT 
      and Cell Titer Blue methods were used to evaluate cell viability. Genotoxicity 
      was measured by the comet assay and the cytokinesis-block micronucleus cytome 
      (CBMN-Cyt) assay, and fluorescence in situ hybridization (FISH) was used to test 
      induction of structural chromosome aberrations (clastogenic activity) and/or 
      numerical chromosome changes (aneuploidogenic activity). Exogenous metabolic 
      activation enzymes were used in the CBMN-Cyt and FISH tests. Only with metabolic 
      activation, the hybrids caused chromosomal damage, by both clastogenic and 
      aneugenic processes.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Ferreira Dantas, Graziela de Paula
AU  - Ferreira Dantas GP
AD  - ToxLab, Departamento de Analises Clinicas e Toxicologicas, Faculdade de Farmacia 
      - Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil. 
      Electronic address: grazi.pfd@gmail.com.
FAU - Nascimento Martins, Estefania Mara do
AU  - Nascimento Martins EMD
AD  - Laboratorio de Quimica de Nanoestruturas de Carbono, Centro de Desenvolvimento da 
      Tecnologia Nuclear (CDTN), Belo Horizonte, MG, Brazil.
FAU - Gomides, Livia Santos
AU  - Gomides LS
AD  - Laboratorio de Quimica de Nanoestruturas de Carbono, Centro de Desenvolvimento da 
      Tecnologia Nuclear (CDTN), Belo Horizonte, MG, Brazil.
FAU - Chequer, Farah Maria Drumond
AU  - Chequer FMD
AD  - Laboratorio de Analises Toxicologicas, Universidade Federal de Sao Joao del-Rei, 
      Campus Centro-Oeste Dona Lindu (UFSJ-CCO), Divinopolis, MG, Brazil.
FAU - Burbano, Rommel Rodriguez
AU  - Burbano RR
AD  - Laboratorio de Citogenetica Humana, Instituto de Ciencias Biologicas, 
      Universidade Federal do Para (UFPA), Belem, PA, Brazil.
FAU - Furtado, Clascidia Aparecida
AU  - Furtado CA
AD  - Laboratorio de Quimica de Nanoestruturas de Carbono, Centro de Desenvolvimento da 
      Tecnologia Nuclear (CDTN), Belo Horizonte, MG, Brazil.
FAU - Santos, Adelina Pinheiro
AU  - Santos AP
AD  - Laboratorio de Quimica de Nanoestruturas de Carbono, Centro de Desenvolvimento da 
      Tecnologia Nuclear (CDTN), Belo Horizonte, MG, Brazil.
FAU - Tagliati, Carlos Alberto
AU  - Tagliati CA
AD  - ToxLab, Departamento de Analises Clinicas e Toxicologicas, Faculdade de Farmacia 
      - Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
LA  - eng
PT  - Journal Article
DEP - 20220211
PL  - Netherlands
TA  - Mutat Res Genet Toxicol Environ Mutagen
JT  - Mutation research. Genetic toxicology and environmental mutagenesis
JID - 101632149
RN  - 0 (Mutagens)
RN  - 0 (Nanotubes, Carbon)
RN  - 0 (Pyrenes)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
SB  - IM
MH  - Animals
MH  - Cricetinae
MH  - Fibroblasts
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Mutagens/toxicity
MH  - *Nanotubes, Carbon/toxicity
MH  - Polyethylene Glycols/toxicity
MH  - Pyrenes/toxicity
OTO - NOTNLM
OT  - Comet assay
OT  - Micronucleus assayMetabolic activation
OT  - Viability
EDAT- 2022/04/29 06:00
MHDA- 2022/05/03 06:00
CRDT- 2022/04/28 21:03
PHST- 2021/09/01 00:00 [received]
PHST- 2022/02/04 00:00 [revised]
PHST- 2022/02/05 00:00 [accepted]
PHST- 2022/04/28 21:03 [entrez]
PHST- 2022/04/29 06:00 [pubmed]
PHST- 2022/05/03 06:00 [medline]
AID - S1383-5718(22)00024-9 [pii]
AID - 10.1016/j.mrgentox.2022.503463 [doi]
PST - ppublish
SO  - Mutat Res Genet Toxicol Environ Mutagen. 2022 Apr-May;876-877:503463. doi: 
      10.1016/j.mrgentox.2022.503463. Epub 2022 Feb 11.