PMID- 35487365 OWN - NLM STAT- MEDLINE DCOM- 20220517 LR - 20220816 IS - 1872-7492 (Electronic) IS - 0168-1702 (Linking) VI - 315 DP - 2022 Jul 2 TI - Minor and major envelope proteins of PRRSV play synergistic roles in inducing heterologous neutralizing antibodies and conferring cross protection. PG - 198789 LID - S0168-1702(22)00116-2 [pii] LID - 10.1016/j.virusres.2022.198789 [doi] AB - High genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) isolates is a major obstacle for the development of effective PRRS vaccines. A chimeric highly pathogenic PRRSV2 (HP-PRRSV2) strain containing the consensus sequence of ORF2-6 genes was constructed in our previous study, which could induce broadly neutralizing antibodies (bnAbs) and confer satisfied cross protection against virulent NADC30-like isolate. To further elucidate the roles of minor and major envelope proteins encoded by ORF2-4 and ORF5-6 genes in conferring cross protection, two chimeric HP-PRRSV2 strains (rJS-ORF2-4-CON and rJS-ORF5-6-CON) containing consensus sequences of ORF2-4 or ORF5-6 were constructed and rescued in this study. The rJS-ORF5-6-CON strain has similar replication efficiency as the backbone HP-PRRSV2 rJSTZ1712-12 virus, while rJS-ORF2-4-CON has significantly lower in vitro and in vivo replication efficiency comparing to rJS-ORF5-6-CON. Animal inoculation indicated that both rJS-ORF2-4-CON and rJS-ORF5-6-CON did not cause obvious clinical signs in piglets and could induce heterologous nAbs after immunization. Challenge with a virulent heterologous NADC30-like SD17-38 isolate showed that even though both immunized groups presented lower viremia, faster virus elimination, less fever and alleviated lung gross lesions when compared with the only challenged pigs, rJS-ORF2-4-CON and rJS-ORF5-6-CON could not confer enough cross protection. Considering the bnAbs and satisfied cross protection induced by the chimeric virus containing ORF2-6 consensus sequence, our results support that minor and major envelope proteins play synergistic roles in inducing broader nAbs and conferring better cross protection. CI - Copyright (c) 2022. Published by Elsevier B.V. FAU - Li, Jixiang AU - Li J AD - College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China. FAU - Li, Shubin AU - Li S AD - College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China. FAU - Qiu, Ming AU - Qiu M AD - College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China. FAU - Li, Xinshuai AU - Li X AD - College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China. FAU - Li, Chen AU - Li C AD - College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China. FAU - Feng, Binghui AU - Feng B AD - College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China. FAU - Lin, Hong AU - Lin H AD - College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China. FAU - Zheng, Wanglong AU - Zheng W AD - College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China; Joint International Research Laboratories of Agriculture and Agri-Product Safety, Yangzhou, Jiangsu 225009, China; Comparative Medicine Research Institute, Yangzhou University, Yangzhou, Jiangsu 225009, China; Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. FAU - Zhu, Jianzhong AU - Zhu J AD - College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China; Joint International Research Laboratories of Agriculture and Agri-Product Safety, Yangzhou, Jiangsu 225009, China; Comparative Medicine Research Institute, Yangzhou University, Yangzhou, Jiangsu 225009, China; Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. Electronic address: jzzhu@yzu.edu.cn. FAU - Chen, Nanhua AU - Chen N AD - College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu 225009, China; Joint International Research Laboratories of Agriculture and Agri-Product Safety, Yangzhou, Jiangsu 225009, China; Comparative Medicine Research Institute, Yangzhou University, Yangzhou, Jiangsu 225009, China; Jiangsu Key Laboratory of Zoonosis, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. Electronic address: hnchen@yzu.edu.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20220426 PL - Netherlands TA - Virus Res JT - Virus research JID - 8410979 RN - 0 (Antibodies, Neutralizing) RN - 0 (Antibodies, Viral) RN - 0 (Broadly Neutralizing Antibodies) RN - 0 (Viral Vaccines) SB - IM MH - Animals MH - Antibodies, Neutralizing MH - Antibodies, Viral MH - Broadly Neutralizing Antibodies MH - Cross Protection MH - *Porcine Reproductive and Respiratory Syndrome/prevention & control MH - *Porcine respiratory and reproductive syndrome virus/genetics MH - Swine MH - *Viral Vaccines/genetics OTO - NOTNLM OT - Chimeric PRRSV OT - Cross protection OT - Heterologous neutralizing antibodies OT - Major envelope proteins OT - Minor envelope proteins EDAT- 2022/04/30 06:00 MHDA- 2022/05/18 06:00 CRDT- 2022/04/29 19:25 PHST- 2022/02/20 00:00 [received] PHST- 2022/04/14 00:00 [revised] PHST- 2022/04/23 00:00 [accepted] PHST- 2022/04/30 06:00 [pubmed] PHST- 2022/05/18 06:00 [medline] PHST- 2022/04/29 19:25 [entrez] AID - S0168-1702(22)00116-2 [pii] AID - 10.1016/j.virusres.2022.198789 [doi] PST - ppublish SO - Virus Res. 2022 Jul 2;315:198789. doi: 10.1016/j.virusres.2022.198789. Epub 2022 Apr 26.