PMID- 35490308
OWN - NLM
STAT- MEDLINE
DCOM- 20220714
LR  - 20220921
IS  - 1523-4681 (Electronic)
IS  - 0884-0431 (Linking)
VI  - 37
IP  - 7
DP  - 2022 Jul
TI  - Loss of Parathyroid Hormone Receptor Signaling in Osteoprogenitors Is Associated 
      With Accumulation of Multiple Hematopoietic Lineages in the Bone Marrow.
PG  - 1321-1334
LID - 10.1002/jbmr.4568 [doi]
AB  - Osteoblasts and their progenitors play an important role in the support of 
      hematopoiesis within the bone marrow (BM) microenvironment. We have previously 
      reported that parathyroid hormone receptor (PTH1R) signaling in osteoprogenitors 
      is required for normal B cell precursor differentiation, and for trafficking of 
      maturing B cells out of the BM. Cells of the osteoblast lineage have been 
      implicated in the regulation of several other hematopoietic cell populations, but 
      the effects of PTH1R signaling in osteoprogenitors on other maturing 
      hematopoietic populations have not been investigated. Here we report that numbers 
      of maturing myeloid, T cell, and erythroid populations were increased in the BM 
      of mice lacking PTH1R in Osx-expressing osteoprogenitors (PTH1R-OsxKO mice; 
      knockout [KO]). This increase in maturing hematopoietic populations was not 
      associated with an increase in progenitor populations or proliferation. The 
      spleens of PTH1R-OsxKO mice were small with decreased numbers of all 
      hematopoietic populations, suggesting that trafficking of mature hematopoietic 
      populations between BM and spleen is impaired in the absence of PTH1R in 
      osteoprogenitors. RNA sequencing (RNAseq) of osteoprogenitors and their 
      descendants in bone and BM revealed increased expression of vascular cell 
      adhesion protein 1 (VCAM-1) and C-X-C motif chemokine ligand 12 (CXCL12), factors 
      that are involved in trafficking of several hematopoietic populations. (c) 2022 
      American Society for Bone and Mineral Research (ASBMR).
CI  - (c) 2022 American Society for Bone and Mineral Research (ASBMR).
FAU - Kimura, Takaharu
AU  - Kimura T
AD  - Department of Medicine (Endocrinology), Stanford University School of Medicine, 
      Stanford, CA, USA.
FAU - Panaroni, Cristina
AU  - Panaroni C
AD  - Department of Medicine (Endocrinology), Stanford University School of Medicine, 
      Stanford, CA, USA.
FAU - Rankin, Erinn B
AU  - Rankin EB
AD  - Department of Radiation Oncology, Stanford University School of Medicine, 
      Stanford, CA, USA.
FAU - Purton, Louise E
AU  - Purton LE
AUID- ORCID: 0000-0001-6593-3168
AD  - St Vincent's Institute of Medical Research, Fitzroy, VIC, Australia.
AD  - The University of Melbourne, Department of Medicine at St Vincent's Hospital, 
      Fitzroy, VIC, Australia.
FAU - Wu, Joy Y
AU  - Wu JY
AUID- ORCID: 0000-0002-1897-4503
AD  - Department of Medicine (Endocrinology), Stanford University School of Medicine, 
      Stanford, CA, USA.
LA  - eng
GR  - GNT1003339/National Health and Medical Research Council/
GR  - R01 AR073773/NH/NIH HHS/United States
GR  - R56 DK112869/NH/NIH HHS/United States
GR  - Victorian State Government Operational Infrastructure Support Program/
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20220601
PL  - England
TA  - J Bone Miner Res
JT  - Journal of bone and mineral research : the official journal of the American 
      Society for Bone and Mineral Research
JID - 8610640
RN  - 0 (PTH1R protein, mouse)
RN  - 0 (Receptor, Parathyroid Hormone, Type 1)
SB  - IM
MH  - Animals
MH  - *Bone Marrow/metabolism
MH  - Bone Marrow Cells/metabolism
MH  - *Hematopoietic Stem Cells/metabolism
MH  - Mice
MH  - *Osteoblasts/metabolism
MH  - *Receptor, Parathyroid Hormone, Type 1/genetics/metabolism
MH  - Signal Transduction
OTO - NOTNLM
OT  - GENETIC ANIMAL MODELS
OT  - OSTEOBLASTS
OT  - OSTEOIMMUNOLOGY
OT  - PTH/VIT D/FGF23
OT  - STROMAL/STEM CELLS
EDAT- 2022/05/02 06:00
MHDA- 2022/07/15 06:00
CRDT- 2022/05/01 03:12
PHST- 2022/04/20 00:00 [revised]
PHST- 2021/10/19 00:00 [received]
PHST- 2022/04/26 00:00 [accepted]
PHST- 2022/05/02 06:00 [pubmed]
PHST- 2022/07/15 06:00 [medline]
PHST- 2022/05/01 03:12 [entrez]
AID - 10.1002/jbmr.4568 [doi]
PST - ppublish
SO  - J Bone Miner Res. 2022 Jul;37(7):1321-1334. doi: 10.1002/jbmr.4568. Epub 2022 Jun 
      1.