PMID- 35491823
OWN - NLM
STAT- MEDLINE
DCOM- 20220520
LR  - 20221104
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Print)
IS  - 0095-1137 (Linking)
VI  - 60
IP  - 5
DP  - 2022 May 18
TI  - Diagnosis of Congenital Toxoplasmosis: Performance of Four IgG and IgM Automated 
      Assays at Birth in a Tricentric Evaluation.
PG  - e0011522
LID - 10.1128/jcm.00115-22 [doi]
LID - e00115-22
AB  - For postnatal diagnosis of congenital toxoplasmosis (CT), the gold standard for 
      the detection of anti-Toxoplasma IgM in newborns relies on the immunosorbent 
      agglutination assay (ISAGA), which is manufactured from whole Toxoplasma 
      parasites that become difficult to maintain. For IgG, only the Platelia assay 
      provides a validated assay for cord blood according to the manufacturer, allowing 
      its use in this context. We compared the analytical performance of four 
      commercialized automated assays, Platelia, Abbott, Vidas, and Liaison, for the 
      detection of IgG and IgM in the cord blood or peripheral blood of newborns from 
      women infected during pregnancy. The assays were performed on samples from 509 
      newborns, collected from the university hospitals of Montpellier, Nimes, and 
      Toulouse. For IgM, the four assays appeared to be sufficiently informative to be 
      used for congenital toxoplasmosis diagnosis (area under the curve [AUC] > 0.8, 
      receiver operating characteristic [ROC] analysis), with Platelia showing the best 
      performance, similar to ISAGA with regard to accuracy (83%). For the Vidas (76%), 
      Abbott (75%), and Liaison (74%) assays, the accuracy was significantly lower. 
      Maternal treatment significantly decreased the sensitivity of all the assays. For 
      IgG, the four evaluated assays showed a sensitivity of over 90%, with Abbott 
      (95%) and Liaison (94%), exhibiting a significantly higher sensitivity than 
      Platelia (90%). Furthermore, Abbott showed its superiority in the cases of 
      maternal infection during the third trimester. In the context of the newborns of 
      mothers infected by Toxoplasma gondii during pregnancy, to ensure efficient care, 
      Platelia and Abbott seemed to be the most suitable reference tests for the 
      detection of IgM for the former and IgG for the latter.
FAU - Avignon, Marine
AU  - Avignon M
AD  - Service de Parasitologie-Mycologie, Universite Toulouse 3, Centre Hospitalier 
      Universitaire de Toulouse, Toulouse, France.
FAU - Leveque, Maude F
AU  - Leveque MF
AD  - Departement de Parasitologie-Mycologie, Universite de Montpellier, Centre 
      Hospitalier Universitaire de Montpellier, Montpellier, France.
AD  - Universite de Montpellier, CNRS, IRD, UMR MiVEGEC, Montpellier, France.
FAU - Guemas, Emilie
AU  - Guemas E
AD  - Service de Parasitologie-Mycologie, Universite Toulouse 3, Centre Hospitalier 
      Universitaire de Toulouse, Toulouse, France.
FAU - Sasso, Milene
AU  - Sasso M
AUID- ORCID: 0000-0002-6539-8545
AD  - Universite de Montpellier, CNRS, IRD, UMR MiVEGEC, Montpellier, France.
AD  - Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire de 
      Nimesgrid.411165.6, Nimes, France.
FAU - Albaba, Sahar
AU  - Albaba S
AD  - Departement de Parasitologie-Mycologie, Universite de Montpellier, Centre 
      Hospitalier Universitaire de Montpellier, Montpellier, France.
FAU - Lachaud, Laurence
AU  - Lachaud L
AD  - Departement de Parasitologie-Mycologie, Universite de Montpellier, Centre 
      Hospitalier Universitaire de Montpellier, Montpellier, France.
AD  - Universite de Montpellier, CNRS, IRD, UMR MiVEGEC, Montpellier, France.
FAU - Fillaux, Judith
AU  - Fillaux J
AUID- ORCID: 0000-0002-5277-189X
AD  - Service de Parasitologie-Mycologie, Universite Toulouse 3, Centre Hospitalier 
      Universitaire de Toulouse, Toulouse, France.
AD  - RESTORE Research Center, Universite de Toulouse, INSERM 1301, CNRS 5070, EFS, 
      ENVT, Toulouse, France.
LA  - eng
PT  - Journal Article
DEP - 20220502
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Antibodies, Protozoan)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulin M)
SB  - IM
MH  - Antibodies, Protozoan
MH  - Female
MH  - Humans
MH  - Immunoglobulin G
MH  - Immunoglobulin M
MH  - Infant, Newborn
MH  - Pregnancy
MH  - *Pregnancy Complications, Parasitic/diagnosis
MH  - *Toxoplasma
MH  - *Toxoplasmosis/diagnosis
MH  - *Toxoplasmosis, Congenital/diagnosis
PMC - PMC9116181
OTO - NOTNLM
OT  - Toxoplasma gondii
OT  - immunodiagnosis
OT  - maternal seroconversion
OT  - newborns
OT  - postnatal diagnosis
COIS- The authors declare no conflict of interest.
EDAT- 2022/05/03 06:00
MHDA- 2022/05/21 06:00
PMCR- 2022/11/02
CRDT- 2022/05/02 04:33
PHST- 2022/05/03 06:00 [pubmed]
PHST- 2022/05/21 06:00 [medline]
PHST- 2022/05/02 04:33 [entrez]
PHST- 2022/11/02 00:00 [pmc-release]
AID - 00115-22 [pii]
AID - jcm.00115-22 [pii]
AID - 10.1128/jcm.00115-22 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2022 May 18;60(5):e0011522. doi: 10.1128/jcm.00115-22. Epub 
      2022 May 2.