PMID- 35491840
OWN - NLM
STAT- MEDLINE
DCOM- 20220520
LR  - 20221104
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Print)
IS  - 0095-1137 (Linking)
VI  - 60
IP  - 5
DP  - 2022 May 18
TI  - Diagnosis of Congenital Toxoplasmosis: No Benefit of IgA Antibody Detection by 
      Platelia ELISA in a Tricentric Evaluation.
PG  - e0011622
LID - 10.1128/jcm.00116-22 [doi]
LID - e00116-22
AB  - The diagnostic accuracy of a commercial Toxoplasma gondii IgA antibody 
      enzyme-linked immunosorbent assay (ELISA) was evaluated in the context of routine 
      practice on 289 newborns with congenital toxoplasmosis (CT) and 220 healthy 
      controls. The performance of this assay was compared to that of the current 
      gold-standard test for anti-Toxoplasma IgM detection, an immunosorbent 
      agglutination assay (ISAGA). IgM and IgA sensitivity and specificity were 
      assessed in cord and postnatal samples. The sensitivity of IgA detection by ELISA 
      on all serum and peripheral blood samples was 60.56% and 56.52%, respectively, 
      which is low compared with the sensitivity of IgM detection by ISAGA (73.26% on 
      serum samples, 82.35% on peripheral blood). Adding the T. gondii IgA antibody 
      ELISA to the diagnostic panel did not significantly increase the overall 
      performance of the serological diagnosis based on IgM detection.
FAU - Leveque, Maude F
AU  - Leveque MF
AD  - Universite de Montpellier, Centre National de la Recherche Scientifique, IRD, UMR 
      MiVEGEC, Montpellier, France.
AD  - Departement de Parasitologie-Mycologie, Centre Hospitalier Universitaire de 
      Montpellier, Montpellier, France.
FAU - Albaba, Sahar
AU  - Albaba S
AD  - Departement de Parasitologie-Mycologie, Centre Hospitalier Universitaire de 
      Montpellier, Montpellier, France.
FAU - Arrada, Neila
AU  - Arrada N
AD  - Departement de Parasitologie-Mycologie, Centre Hospitalier Universitaire de 
      Montpellier, Montpellier, France.
FAU - Avignon, Marine
AU  - Avignon M
AD  - Service de Parasitologie-Mycologie, Centre Hospitalier Universitaire de Toulouse, 
      Toulouse, France.
FAU - Sasso, Milene
AU  - Sasso M
AUID- ORCID: 0000-0002-6539-8545
AD  - Universite de Montpellier, Centre National de la Recherche Scientifique, IRD, UMR 
      MiVEGEC, Montpellier, France.
AD  - Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire de 
      Nimesgrid.411165.6, Nimes, France.
FAU - Fillaux, Judith
AU  - Fillaux J
AD  - Service de Parasitologie-Mycologie, Centre Hospitalier Universitaire de Toulouse, 
      Toulouse, France.
AD  - RESTORE Research Center, Universite de Toulouse, Inserm, Centre National de la 
      Recherche Scientifique, EFS, ENVT, Toulouse, France.
FAU - Lachaud, Laurence
AU  - Lachaud L
AD  - Universite de Montpellier, Centre National de la Recherche Scientifique, IRD, UMR 
      MiVEGEC, Montpellier, France.
AD  - Departement de Parasitologie-Mycologie, Centre Hospitalier Universitaire de 
      Montpellier, Montpellier, France.
LA  - eng
PT  - Journal Article
DEP - 20220502
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (Antibodies, Protozoan)
RN  - 0 (Immunoglobulin A)
RN  - 0 (Immunoglobulin M)
SB  - IM
CIN - J Clin Microbiol. 60.
MH  - Antibodies, Protozoan
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Immunoglobulin A
MH  - Immunoglobulin M
MH  - Infant, Newborn
MH  - *Toxoplasma
MH  - *Toxoplasmosis/diagnosis
MH  - *Toxoplasmosis, Congenital/diagnosis
PMC - PMC9116159
OTO - NOTNLM
OT  - IgM
OT  - Toxoplasma gondii
OT  - immunodiagnosis
OT  - maternal seroconversion
OT  - newborns
OT  - postnatal diagnosis
COIS- The authors declare no conflict of interest. We have no competing interests to 
      declare.
EDAT- 2022/05/03 06:00
MHDA- 2022/05/21 06:00
PMCR- 2022/11/02
CRDT- 2022/05/02 04:43
PHST- 2022/05/03 06:00 [pubmed]
PHST- 2022/05/21 06:00 [medline]
PHST- 2022/05/02 04:43 [entrez]
PHST- 2022/11/02 00:00 [pmc-release]
AID - 00116-22 [pii]
AID - jcm.00116-22 [pii]
AID - 10.1128/jcm.00116-22 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2022 May 18;60(5):e0011622. doi: 10.1128/jcm.00116-22. Epub 
      2022 May 2.