PMID- 35497743
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220503
IS  - 2046-2069 (Electronic)
IS  - 2046-2069 (Linking)
VI  - 10
IP  - 6
DP  - 2020 Jan 16
TI  - Tryptophan promoted beta-defensin-2 expression via the mTOR pathway and its 
      metabolites: kynurenine banding to aryl hydrocarbon receptor in rat intestine.
PG  - 3371-3379
LID - 10.1039/c9ra10477a [doi]
AB  - In this study, we investigated the signalling pathways mediating tryptophan 
      (Trp)-promoted beta-defensin-2 (BD-2) expression in rat intestinal mucosa. Sprague 
      Dawley rats were administered with l-Trp and treated with rapamycin (RAPA), 
      1-methyltryptophan (1-MT), or para-chlorophenyl-amine (PCPA) to inhibit mammalian 
      target of rapamycin (mTOR), indoleamine-2,3-dioxygenase (IDO), or tryptophan 
      hydroxylase (TPH), respectively. The mRNA and protein levels of BD-2 in the 
      jejunal and ileal mucosa of rats increased with administration of l-Trp. 
      Intraperitoneal injection of RAPA significantly decreased the mRNA level of BD-2 
      and the concentrations of p-mTORC1 and BD-2 in the jejunal and ileal mucosa of 
      rats with administration of l-Trp (P < 0.05). Oral administration of 1-MT 
      decreased the IDO activity and the mRNA and protein levels of BD-2, and increased 
      the concentrations of tumour necrosis factor (TNF-alpha), interleukin (IL)-17, and 
      IL-22 in the jejunal and ileal mucosa of rats with administration of l-Trp (P < 
      0.05). Intraperitoneal injection of PCPA decreased the TPH activity and increased 
      the mRNA and protein levels of BD-2, but did not change the concentrations of 
      TNF-alpha, IL-17, or IL-22 in the jejunal and ileal mucosa of rats with 
      administration of l-Trp. The results indicate the Trp-promoted BD-2 expression in 
      the jejunum and ileum via the mTOR pathway and its metabolites: kynurenine 
      banding to aryl hydrocarbon receptor in rat intestine.
CI  - This journal is (c) The Royal Society of Chemistry.
FAU - Tang, Zhiru
AU  - Tang Z
AD  - Laboratory for Bio-feed and Molecular Nutrition, College of Animal Science and 
      Technology, Southwest University Chongqing 400715 China sunzh2002cn@aliyun.com.
FAU - Shi, Baoshi
AU  - Shi B
AD  - Laboratory for Bio-feed and Molecular Nutrition, College of Animal Science and 
      Technology, Southwest University Chongqing 400715 China sunzh2002cn@aliyun.com.
FAU - Sun, Weizhong
AU  - Sun W
AD  - Laboratory for Bio-feed and Molecular Nutrition, College of Animal Science and 
      Technology, Southwest University Chongqing 400715 China sunzh2002cn@aliyun.com.
FAU - Yin, Yulong
AU  - Yin Y
AD  - Institute of Subtropical Agriculture, The Chinese Academy of Sciences Changsha 
      410125 P. R. China.
FAU - Chen, Qingju
AU  - Chen Q
AD  - Laboratory for Bio-feed and Molecular Nutrition, College of Animal Science and 
      Technology, Southwest University Chongqing 400715 China sunzh2002cn@aliyun.com.
FAU - Mohamed, Taha
AU  - Mohamed T
AD  - Laboratory for Bio-feed and Molecular Nutrition, College of Animal Science and 
      Technology, Southwest University Chongqing 400715 China sunzh2002cn@aliyun.com.
FAU - Lu, Changwen
AU  - Lu C
AD  - Laboratory for Bio-feed and Molecular Nutrition, College of Animal Science and 
      Technology, Southwest University Chongqing 400715 China sunzh2002cn@aliyun.com.
FAU - Sun, Zhihong
AU  - Sun Z
AUID- ORCID: 0000-0002-3839-037X
AD  - Laboratory for Bio-feed and Molecular Nutrition, College of Animal Science and 
      Technology, Southwest University Chongqing 400715 China sunzh2002cn@aliyun.com.
LA  - eng
PT  - Journal Article
DEP - 20200121
PL  - England
TA  - RSC Adv
JT  - RSC advances
JID - 101581657
PMC - PMC9049194
COIS- There are no conflicts to declare.
EDAT- 2020/01/21 00:00
MHDA- 2020/01/21 00:01
PMCR- 2020/01/21
CRDT- 2022/05/02 07:04
PHST- 2019/12/13 00:00 [received]
PHST- 2020/01/04 00:00 [accepted]
PHST- 2022/05/02 07:04 [entrez]
PHST- 2020/01/21 00:00 [pubmed]
PHST- 2020/01/21 00:01 [medline]
PHST- 2020/01/21 00:00 [pmc-release]
AID - c9ra10477a [pii]
AID - 10.1039/c9ra10477a [doi]
PST - epublish
SO  - RSC Adv. 2020 Jan 21;10(6):3371-3379. doi: 10.1039/c9ra10477a. eCollection 2020 
      Jan 16.