PMID- 35498659
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220503
IS  - 1664-462X (Print)
IS  - 1664-462X (Electronic)
IS  - 1664-462X (Linking)
VI  - 13
DP  - 2022
TI  - OsbZIP47 Is an Integrator for Meristem Regulators During Rice Plant Growth and 
      Development.
PG  - 865928
LID - 10.3389/fpls.2022.865928 [doi]
LID - 865928
AB  - Stem cell homeostasis by the WUSCHEL-CLAVATA (WUS-CLV) feedback loop is generally 
      conserved across species; however, its links with other meristem regulators can 
      be species-specific, rice being an example. We characterized the role of rice 
      OsbZIP47 in vegetative and reproductive development. The knockdown (KD) 
      transgenics showed meristem size abnormality and defects in developmental 
      progression. The size of the shoot apical meristem (SAM) in 25-day OsbZIP47KD 
      plants was increased as compared to the wild-type (WT). Inflorescence of KD 
      plants showed reduced rachis length, number of primary branches, and spikelets. 
      Florets had defects in the second and third whorl organs and increased organ 
      number. OsbZIP47KD SAM and panicles had abnormal expression for CLAVATA 
      peptide-like signaling genes, such as FON2-LIKE CLE PROTEIN1 (FCP1), FLORAL ORGAN 
      NUMBER 2 (FON2), and hormone pathway genes, such as cytokinin (CK) 
      ISOPENTEYLTRANSFERASE1 (OsIPT1), ISOPENTEYLTRANSFERASE 8 (OsIPT8), auxin 
      biosynthesis OsYUCCA6, OsYUCCA7 and gibberellic acid (GA) biosynthesis genes, 
      such as GRAIN NUMBER PER PANICLE1 (GNP1/OsGA20OX1) and SHORTENED BASAL INTERNODE 
      (SBI/OsGA2ox4). The effects on ABBERANT PANICLE ORGANIZATION1 (APO1), OsMADS16, 
      and DROOPING LEAF (DL) relate to the second and third whorl floret phenotypes in 
      OsbZIP47KD. Protein interaction assays showed OsbZIP47 partnerships with RICE 
      HOMEOBOX1 (OSH1), RICE FLORICULA/LEAFY (RFL), and OsMADS1 transcription factors. 
      The meta-analysis of KD panicle transcriptomes in OsbZIP47KD, OsMADS1KD, and 
      RFLKD transgenics, combined with global OSH1 binding sites divulge potential 
      targets coregulated by OsbZIP47, OsMADS1, OSH1, and RFL. Further, we demonstrate 
      that OsbZIP47 redox status affects its DNA binding affinity to a cis element in 
      FCP1, a target locus. Taken together, we provide insights on OsbZIP47 roles in 
      SAM development, inflorescence branching, and floret development.
CI  - Copyright (c) 2022 Prakash, Rai, Zamzam, Ahmad, Peesapati and Vijayraghavan.
FAU - Prakash, Sandhan
AU  - Prakash S
AD  - Department of Microbiology and Cell Biology, Indian Institute of Science, 
      Bengaluru, India.
FAU - Rai, Rashmi
AU  - Rai R
AD  - Department of Microbiology and Cell Biology, Indian Institute of Science, 
      Bengaluru, India.
FAU - Zamzam, Mohamed
AU  - Zamzam M
AD  - Department of Microbiology and Cell Biology, Indian Institute of Science, 
      Bengaluru, India.
FAU - Ahmad, Owais
AU  - Ahmad O
AD  - Department of Microbiology and Cell Biology, Indian Institute of Science, 
      Bengaluru, India.
FAU - Peesapati, Raghavaram
AU  - Peesapati R
AD  - Department of Microbiology and Cell Biology, Indian Institute of Science, 
      Bengaluru, India.
FAU - Vijayraghavan, Usha
AU  - Vijayraghavan U
AD  - Department of Microbiology and Cell Biology, Indian Institute of Science, 
      Bengaluru, India.
LA  - eng
PT  - Journal Article
DEP - 20220413
PL  - Switzerland
TA  - Front Plant Sci
JT  - Frontiers in plant science
JID - 101568200
PMC - PMC9044032
OTO - NOTNLM
OT  - FASCIATED EAR4
OT  - Oryza sativa
OT  - OsMADS1
OT  - PERIANTHIA
OT  - floret
OT  - panicle
OT  - shoot apical meristem
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/05/03 06:00
MHDA- 2022/05/03 06:01
PMCR- 2022/01/01
CRDT- 2022/05/02 07:14
PHST- 2022/01/30 00:00 [received]
PHST- 2022/03/09 00:00 [accepted]
PHST- 2022/05/02 07:14 [entrez]
PHST- 2022/05/03 06:00 [pubmed]
PHST- 2022/05/03 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fpls.2022.865928 [doi]
PST - epublish
SO  - Front Plant Sci. 2022 Apr 13;13:865928. doi: 10.3389/fpls.2022.865928. 
      eCollection 2022.