PMID- 35499493
OWN - NLM
STAT- MEDLINE
DCOM- 20220526
LR  - 20220531
IS  - 1530-6992 (Electronic)
IS  - 1530-6984 (Linking)
VI  - 22
IP  - 10
DP  - 2022 May 25
TI  - Fluorescent Artificial Antigens Revealed Extended Membrane Networks Utilized by 
      Live Dendritic Cells for Antigen Uptake.
PG  - 4020-4027
LID - 10.1021/acs.nanolett.2c00629 [doi]
AB  - Dendritic cells (DCs) can infiltrate tight junctions of the epithelium to collect 
      remote antigens during immune surveillance. While elongated membrane structures 
      represent a plausible structure to perform this task, their functional mechanisms 
      remain elusive owing to the lack of high-resolution characterizations in live 
      DCs. Here, we developed fluorescent artificial antigens (FAAs) based on quantum 
      dots coated with polyacrylic acid. Single-particle tracking of FAAs enables us to 
      superresolve the membrane fiber network responsible for the antigen uptake. Using 
      the DC2.4 cell line as a model system, we discovered the extensive membrane 
      network approaching 200 mum in length with tunnel-like cavities about 150 nm in 
      width. The membrane fiber network also contained heterogeneous circular 
      migrasomes. Disconnecting the membrane network from the cell body decreased the 
      intracellular FAA density. Our study enables mechanistic investigations of DC 
      membrane networks and nanocarriers that target this mechanism.
FAU - Jing, Haoran
AU  - Jing H
AUID- ORCID: 0000-0001-6607-4035
AD  - Department of Chemistry, College of Liberal Arts and Sciences, University of 
      Illinois at Chicago, Chicago, Illinois 60607-7061, United States.
FAU - Saed, Badeia
AU  - Saed B
AD  - Department of Chemistry, College of Liberal Arts and Sciences, University of 
      Illinois at Chicago, Chicago, Illinois 60607-7061, United States.
FAU - Palmai, Marcell
AU  - Palmai M
AD  - Department of Chemistry, College of Liberal Arts and Sciences, University of 
      Illinois at Chicago, Chicago, Illinois 60607-7061, United States.
FAU - Gunasekara, Hirushi
AU  - Gunasekara H
AUID- ORCID: 0000-0002-3750-2309
AD  - Department of Chemistry, College of Liberal Arts and Sciences, University of 
      Illinois at Chicago, Chicago, Illinois 60607-7061, United States.
FAU - Snee, Preston T
AU  - Snee PT
AUID- ORCID: 0000-0002-6544-6826
AD  - Department of Chemistry, College of Liberal Arts and Sciences, University of 
      Illinois at Chicago, Chicago, Illinois 60607-7061, United States.
FAU - Hu, Ying S
AU  - Hu YS
AUID- ORCID: 0000-0002-3912-7063
AD  - Department of Chemistry, College of Liberal Arts and Sciences, University of 
      Illinois at Chicago, Chicago, Illinois 60607-7061, United States.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20220502
PL  - United States
TA  - Nano Lett
JT  - Nano letters
JID - 101088070
RN  - 0 (Antigens)
RN  - 0 (Vaccines, Synthetic)
SB  - IM
MH  - Antigens
MH  - Cell Line
MH  - *Dendritic Cells
MH  - *Quantum Dots
MH  - Vaccines, Synthetic
OTO - NOTNLM
OT  - Dendritic cells
OT  - live-cell superresolution imaging
OT  - membrane fiber
OT  - migrasome
OT  - quantum dots
EDAT- 2022/05/03 06:00
MHDA- 2022/05/27 06:00
CRDT- 2022/05/02 11:20
PHST- 2022/05/03 06:00 [pubmed]
PHST- 2022/05/27 06:00 [medline]
PHST- 2022/05/02 11:20 [entrez]
AID - 10.1021/acs.nanolett.2c00629 [doi]
PST - ppublish
SO  - Nano Lett. 2022 May 25;22(10):4020-4027. doi: 10.1021/acs.nanolett.2c00629. Epub 
      2022 May 2.