PMID- 35504414
OWN - NLM
STAT- MEDLINE
DCOM- 20220518
LR  - 20220518
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 209
IP  - Pt B
DP  - 2022 Jun 1
TI  - Experimental study on fabrication, biocompatibility and mechanical 
      characterization of polyhydroxybutyrate-ball clay bionanocomposites for bone 
      tissue engineering.
PG  - 1995-2008
LID - S0141-8130(22)00892-3 [pii]
LID - 10.1016/j.ijbiomac.2022.04.178 [doi]
AB  - The poly (3-hydroxybutyrate) (PHB)/ball clay nanocomposites (B1-B10) were 
      synthesized using solvent casting method with different weight percentage of ball 
      clay in PHB matrix. Scanning electron microscope (SEM) showed maximum root mean 
      square roughness (188.73 mum) for 10% ball clay loading. Fourier transforms 
      infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) showed establishment of 
      intercalated structure and formation of hydrogen bond between ball clay and PHB 
      matrix. Contact angle values (67.3 - 51.3 degrees ) exhibited that the nanocomposites 
      (B1-B10) are more hydrophilic than neat PHB (70.30 degrees ). Thermogravimetric (TGA) and 
      differential scanning calorimetry (DSC) revealed maximum T(max) (278  degrees C) and T(m) 
      (175  degrees C) for the nanocomposite B10 (PHB/PEG/ball clay: 80%/10%/10%). Maximum 
      tensile strength (38.21 +/- 0.15 MPa) and Young's modulus (1.74 +/- 0.016 GPa) was 
      observed for B10 nanocomposite. The values of protein adsorption, platelet 
      adhesion, PT, APTT and complement activation for B10 nanocomposites were 
      165 +/- 2 mug/cm(2), 72 +/- 3 x 10(9) platelets/cm(2), 23 +/- 1 s, 44 +/- 2 s, 
      102 +/- 2 mg/dL and 631 +/- 3 mg/dL, respectively. Hydroxyapatite formation was also 
      observed for nanocomposite (B10) in in vitro simulated body fluid (SBF) study. 
      Finally, the nanocomposite (B10) showed no harmful effect on MG-63 cells, 
      indicating that they are physiologically safe.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Ankush, K
AU  - Ankush K
AD  - Department of Biotechnology, National institute of Technology Raipur, Raipur, 
      Chhattisgarh 492010, India.
FAU - Pugazhenthi, G
AU  - Pugazhenthi G
AD  - Department of Chemical Engineering, Indian Institute of Technology Guwahati, 
      Guwahati, Assam 781039, India.
FAU - Mohit, K
AU  - Mohit K
AD  - Department of Biotechnology, National institute of Technology Raipur, Raipur, 
      Chhattisgarh 492010, India.
FAU - Vasanth, D
AU  - Vasanth D
AD  - Department of Biotechnology, National institute of Technology Raipur, Raipur, 
      Chhattisgarh 492010, India. Electronic address: dvasanth.bt@nitrr.ac.in.
LA  - eng
PT  - Journal Article
DEP - 20220430
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - T1FAD4SS2M (Clay)
SB  - IM
MH  - Bone and Bones
MH  - Clay/chemistry
MH  - *Nanocomposites/chemistry
MH  - Spectroscopy, Fourier Transform Infrared
MH  - *Tissue Engineering
OTO - NOTNLM
OT  - Ball clay
OT  - Bio-nanocomposites
OT  - Bioactivity
OT  - Hemocompatibility
OT  - Nanocomposite
OT  - PHB
EDAT- 2022/05/04 06:00
MHDA- 2022/05/19 06:00
CRDT- 2022/05/03 19:25
PHST- 2022/02/24 00:00 [received]
PHST- 2022/04/22 00:00 [revised]
PHST- 2022/04/24 00:00 [accepted]
PHST- 2022/05/04 06:00 [pubmed]
PHST- 2022/05/19 06:00 [medline]
PHST- 2022/05/03 19:25 [entrez]
AID - S0141-8130(22)00892-3 [pii]
AID - 10.1016/j.ijbiomac.2022.04.178 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2022 Jun 1;209(Pt B):1995-2008. doi: 
      10.1016/j.ijbiomac.2022.04.178. Epub 2022 Apr 30.