PMID- 35504483 OWN - NLM STAT- MEDLINE DCOM- 20220602 LR - 20220622 IS - 1873-6815 (Electronic) IS - 0531-5565 (Linking) VI - 164 DP - 2022 Jul TI - Individual evaluation of aging- and caloric restriction-related changes to distinct multimeric complexes of circulating adiponectin by immunoblotting. PG - 111821 LID - S0531-5565(22)00129-2 [pii] LID - 10.1016/j.exger.2022.111821 [doi] AB - Adiponectin (APN), a major adipokine secreted from white adipose tissue, prevents inflammation and improves insulin sensitivity. APN exists as distinct multimeric complexes with different physiological activities, including low, middle and high molecular weight complexes (LMW, MMW and HMW, respectively) in peripheral blood. Caloric restriction (CR), an intervention that suppresses aging-related pathophysiological changes and extends lifespan, reportedly elevates the expression levels of Adipoq (encoding APN) and total circulating APN. Circulating APN levels have generally been measured using ELISA, but ELISA fails to directly and separately detect APN multimeric complexes other than HMW. Here, we aimed to evaluate the association of aging and CR with oligomerization of APN in rodent models, using immunoblotting to distinguish multimeric complexes based on molecular sizes. In mice, aging elevated plasma levels of HMW and MMW, while CR only elevated HMW. In contrast, LMW and monomeric APN levels were unchanged, suggesting that aging and CR can induce the assembly of APN oligomers in adipocytes. In rats, plasma levels of all multimeric complexes and monomeric APN were not significantly changed by aging or CR. Collectively, levels of circulating APN in mice were consistent with previous findings, whereas those of rats were partially inconsistent, probably because of experimental differences. Moreover, aging reduced Adipoq mRNA levels in mice and rats, while CR prevented this reduction only in rats. Such a discrepancy between Adipoq expression and circulating APN levels may be attributed to proteasomal regulation in adipocytes or tissue accumulation of APN. In conclusion, this study provides new findings of aging- and CR-related changes of each APN multimeric complex and underscores the importance of qualitative approaches for a greater understanding of physiological changes in APN. CI - Copyright (c) 2022 The Author(s). Published by Elsevier Inc. All rights reserved. FAU - Kobayashi, Masaki AU - Kobayashi M AD - Laboratory of Molecular Pathology and Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan. Electronic address: kobayashim@rs.tus.ac.jp. FAU - Nezu, Yuichiro AU - Nezu Y AD - Laboratory of Molecular Pathology and Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan. FAU - Itoh, Mayu AU - Itoh M AD - Laboratory of Molecular Pathology and Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan. FAU - Uchida, Rio AU - Uchida R AD - Laboratory of Molecular Pathology and Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan. FAU - Arikawa, Tomoya AU - Arikawa T AD - Laboratory of Molecular Pathology and Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan. FAU - Otsubo, Minami AU - Otsubo M AD - Laboratory of Molecular Pathology and Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan. FAU - Nozaki, Yuka AU - Nozaki Y AD - Laboratory of Molecular Pathology and Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan. FAU - Tagawa, Ryoma AU - Tagawa R AD - Laboratory of Molecular Pathology and Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan. FAU - Fujishima, Yuya AU - Fujishima Y AD - Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, 2-2-B5 Yamada-oka, Suita, Osaka 565-0871, Japan. FAU - Maeda, Norikazu AU - Maeda N AD - Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, 2-2-B5 Yamada-oka, Suita, Osaka 565-0871, Japan. FAU - Shimomura, Iichiro AU - Shimomura I AD - Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, 2-2-B5 Yamada-oka, Suita, Osaka 565-0871, Japan. FAU - Higami, Yoshikazu AU - Higami Y AD - Laboratory of Molecular Pathology and Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan; Research Institute for Biomedical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba 278-8510, Japan. Electronic address: higami@rs.tus.ac.jp. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20220430 PL - England TA - Exp Gerontol JT - Experimental gerontology JID - 0047061 RN - 0 (Adiponectin) SB - IM MH - *Adiponectin MH - Aging MH - Animals MH - Caloric Restriction MH - Immunoblotting MH - *Insulin Resistance MH - Mice MH - Overweight MH - Rats OTO - NOTNLM OT - Adiponectin OT - Aging OT - Caloric restriction OT - Immunoblotting EDAT- 2022/05/04 06:00 MHDA- 2022/06/03 06:00 CRDT- 2022/05/03 19:26 PHST- 2021/10/01 00:00 [received] PHST- 2022/04/23 00:00 [revised] PHST- 2022/04/25 00:00 [accepted] PHST- 2022/05/04 06:00 [pubmed] PHST- 2022/06/03 06:00 [medline] PHST- 2022/05/03 19:26 [entrez] AID - S0531-5565(22)00129-2 [pii] AID - 10.1016/j.exger.2022.111821 [doi] PST - ppublish SO - Exp Gerontol. 2022 Jul;164:111821. doi: 10.1016/j.exger.2022.111821. Epub 2022 Apr 30.