PMID- 35507985
OWN - NLM
STAT- MEDLINE
DCOM- 20220506
LR  - 20220716
IS  - 1678-7765 (Electronic)
IS  - 1678-7757 (Print)
IS  - 1678-7757 (Linking)
VI  - 30
DP  - 2022
TI  - Expression of anti-fungal peptide, beta-defensin 118 in oral fibroblasts induced by 
      C. albicans beta-glucan-containing particles.
PG  - e20210321
LID - S1678-77572022000100421 [pii]
LID - 10.1590/1678-7757-2021-0321 [doi]
LID - e20210321
AB  - OBJECTIVE: Although oral fibroblasts are thought to have the potential to enhance 
      host defenses against Candida albicans , it is unknown whether they are able to 
      recognize Candida cell components to increase the expression of antifungal 
      peptides, such as defensin factors, against Candida infection. METHODOLOGY: We 
      performed expression profiles of defensin genes induced by heat-killed C. 
      albicans in oral immortalized fibroblasts (GT1) using cDNA microarray analysis. 
      From those results, quantitative RT-PCR was used to examine the effects of 
      Candida beta-glucan-containing particles (beta-GPs) on beta-Defensin 118 (DEFB 118) 
      expression in oral mucosal cells. Furthermore, the antifungal activities of 
      recombinant DEFB 118 against C. albicans and C. glabrata were investigated using 
      fungicidal assays. RESULTS: Microarray analysis showed that DEFB118, beta-Defensin 
      129 (DEFB129), and alpha-Defensin 1 (DEFA1) genes were induced by heat-killed C. 
      albicans and that their mRNA expressions were also significantly increased by 
      live as well as heat-killed C. albicans . Next, we focused on DEFB118, and found 
      that GT1, primary fibroblasts, and RT7 (oral immortalized keratinocytes) 
      constitutively expressed DEFB118 mRNA expression in RT-PCR. Furthermore, C. 
      albicans beta-GPs significantly increased the expression of DEFB118 mRNA in GT1 and 
      primary fibroblasts. Although DEFB118 mRNA expression in RT7 was significantly 
      induced by both live and heat-killed C. albicans, C. albicans beta-GPs failed to 
      have an effect on that expression. Finally, recombinant DEFB118 significantly 
      decreased the survival of both strains of C. albicans in a dose-dependent manner, 
      whereas no effects were seen for both C. glabrata strains. CONCLUSION: DEFB118, 
      induced by C. albicans beta-GPs from oral fibroblasts, may play an important role in 
      oral immune responses against C. albicans infection.
FAU - Sakuma, Miyuki
AU  - Sakuma M
AD  - Hiroshima University, Graduate School of Biomedical and Health Sciences, 
      Department of Oral and Maxillofacial Surgery, Hiroshima, Japan.
FAU - Ohta, Kouji
AU  - Ohta K
AUID- ORCID: 0000-0002-0603-9171
AD  - Hiroshima University, Graduate School of Biomedical and Health Sciences, Program 
      of Oral Health Sciences, Department of Public Oral Health, Hiroshima, Japan.
FAU - Fukada, Shohei
AU  - Fukada S
AD  - Hiroshima University, Graduate School of Biomedical and Health Sciences, 
      Department of Oral and Maxillofacial Surgery, Hiroshima, Japan.
FAU - Kato, Hiroki
AU  - Kato H
AD  - Hiroshima University, Graduate School of Biomedical and Health Sciences, 
      Department of Oral and Maxillofacial Surgery, Hiroshima, Japan.
FAU - Naruse, Takako
AU  - Naruse T
AD  - Hiroshima University, Graduate School of Biomedical and Health Sciences, 
      Department of Oral and Maxillofacial Surgery, Hiroshima, Japan.
FAU - Nakagawa, Takayuki
AU  - Nakagawa T
AD  - Hiroshima University, Graduate School of Biomedical and Health Sciences, 
      Department of Oral and Maxillofacial Surgery, Hiroshima, Japan.
FAU - Shigeishi, Hideo
AU  - Shigeishi H
AUID- ORCID: 0000-0003-0883-4299
AD  - Hiroshima University, Graduate School of Biomedical and Health Sciences, Program 
      of Oral Health Sciences, Department of Public Oral Health, Hiroshima, Japan.
FAU - Nishi, Hiromi
AU  - Nishi H
AD  - Hiroshima University Hospital, Department of General Dentistry, Hiroshima, Japan.
FAU - Takechi, Masaaki
AU  - Takechi M
AD  - Hiroshima University, Graduate School of Biomedical and Health Sciences, 
      Department of Oral and Maxillofacial Surgery, Hiroshima, Japan.
LA  - eng
PT  - Journal Article
DEP - 20220429
PL  - Brazil
TA  - J Appl Oral Sci
JT  - Journal of applied oral science : revista FOB
JID - 101189774
RN  - 0 (Antifungal Agents)
RN  - 0 (Glucans)
RN  - 0 (RNA, Messenger)
RN  - 0 (beta-Defensins)
RN  - 0 (beta-Glucans)
SB  - IM
MH  - Antifungal Agents/pharmacology
MH  - Candida albicans
MH  - Fibroblasts
MH  - Glucans/metabolism
MH  - RNA, Messenger/metabolism
MH  - *beta-Defensins/genetics/metabolism/pharmacology
MH  - *beta-Glucans/metabolism/pharmacology
PMC - PMC9064192
COIS- Conflict of interest The authors declare no conflict of interest.
EDAT- 2022/05/05 06:00
MHDA- 2022/05/07 06:00
PMCR- 2022/04/29
CRDT- 2022/05/04 17:43
PHST- 2021/06/21 00:00 [received]
PHST- 2022/02/02 00:00 [accepted]
PHST- 2022/05/04 17:43 [entrez]
PHST- 2022/05/05 06:00 [pubmed]
PHST- 2022/05/07 06:00 [medline]
PHST- 2022/04/29 00:00 [pmc-release]
AID - S1678-77572022000100421 [pii]
AID - 10.1590/1678-7757-2021-0321 [doi]
PST - epublish
SO  - J Appl Oral Sci. 2022 Apr 29;30:e20210321. doi: 10.1590/1678-7757-2021-0321. 
      eCollection 2022.