PMID- 35508623
OWN - NLM
STAT- MEDLINE
DCOM- 20220506
LR  - 20220716
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 12
IP  - 1
DP  - 2022 May 4
TI  - Pulsed electromagnetic fields inhibit mandibular bone deterioration depending on 
      the Wnt3a/beta-catenin signaling activation in type 2 diabetic db/db mice.
PG  - 7217
LID - 10.1038/s41598-022-10065-7 [doi]
LID - 7217
AB  - Type 2 diabetes mellitus (T2DM) patients have compromised mandibular bone 
      architecture/quality, which markedly increase the risks of tooth loosening, tooth 
      loss, and failure of dental implantation. However, it remains lacks effective and 
      safe countermeasures against T2DM-related mandibular bone deterioration. Herein, 
      we studied the effects of pulsed electromagnetic fields (PEMF) on mandibular bone 
      microstructure/quality and relevant regulatory mechanisms in T2DM db/db mice. 
      PEMF exposure (20 Gs, 15 Hz) for 12 weeks preserved trabecular bone architecture, 
      increased cortical bone thickness, improved material properties and stimulated 
      bone anabolism in mandibles of db/db mice. PEMF also upregulated the expression 
      of canonical Wnt3a ligand (but not Wnt1 or Wnt5a) and its downstream beta-catenin. 
      PEMF improved the viability and differentiation of primary osteoblasts isolated 
      from the db/db mouse mandible, and stimulated the specific activation of 
      Wnt3a/beta-catenin signaling. These positive effects of PEMF on mandibular 
      osteoblasts of db/db mice were almost totally abolished after Wnt3a silencing in 
      vitro, which were equivalent to the effects following blockade of canonical Wnt 
      signaling using the broad-spectrum antagonist DKK1. Injection with Wnt3a siRNA 
      abrogated the therapeutic effects of PEMF on mandibular bone quantity/quality and 
      bone anabolism in db/db mice. Our study indicates that PEMF might become a 
      non-invasive and safe treatment alternative resisting mandibular bone 
      deterioration in T2DM patients, which is helpful for protecting teeth from 
      loosening/loss and securing the dental implant stability.
CI  - (c) 2022. The Author(s).
FAU - Li, Jianjun
AU  - Li J
AD  - Second Clinical Division, Peking University School and Hospital of Stomatology & 
      National Clinical Research Center for Oral Diseases & National Engineering 
      Laboratory for Digital and Material Technology of Stomatology & Beijing Key 
      Laboratory of Digital Stomatology, 22 Zhongguancun South Avenue, Beijing, 100081, 
      China. dentistlijianjun@163.com.
AD  - Beijing Healya Technology Limited, Beijing, 100195, China. 
      dentistlijianjun@163.com.
FAU - Cai, Jing
AU  - Cai J
AD  - College of Basic Medicine, Shaanxi University of Chinese Medicine, Xianyang, 
      China.
FAU - Liu, Liheng
AU  - Liu L
AD  - Department of Obstetrics, Beijing Obstetrics and Gynecology Hospital, Capital 
      Medical University, Beijing, China.
FAU - Wu, Yuwei
AU  - Wu Y
AD  - Second Clinical Division, Peking University School and Hospital of Stomatology & 
      National Clinical Research Center for Oral Diseases & National Engineering 
      Laboratory for Digital and Material Technology of Stomatology & Beijing Key 
      Laboratory of Digital Stomatology, 22 Zhongguancun South Avenue, Beijing, 100081, 
      China.
FAU - Chen, Yan
AU  - Chen Y
AD  - Second Clinical Division, Peking University School and Hospital of Stomatology & 
      National Clinical Research Center for Oral Diseases & National Engineering 
      Laboratory for Digital and Material Technology of Stomatology & Beijing Key 
      Laboratory of Digital Stomatology, 22 Zhongguancun South Avenue, Beijing, 100081, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20220504
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (WNT3A protein, human)
RN  - 0 (Wnt3A Protein)
RN  - 0 (beta Catenin)
SB  - IM
MH  - Animals
MH  - *Diabetes Mellitus, Type 2/complications/metabolism/therapy
MH  - Electromagnetic Fields
MH  - Humans
MH  - Mandible/metabolism
MH  - Mice
MH  - Wnt Signaling Pathway/physiology
MH  - Wnt3A Protein
MH  - *beta Catenin/metabolism
PMC - PMC9068619
COIS- The authors declare no competing interests.
EDAT- 2022/05/05 06:00
MHDA- 2022/05/07 06:00
PMCR- 2022/05/04
CRDT- 2022/05/04 23:47
PHST- 2021/08/04 00:00 [received]
PHST- 2022/03/01 00:00 [accepted]
PHST- 2022/05/04 23:47 [entrez]
PHST- 2022/05/05 06:00 [pubmed]
PHST- 2022/05/07 06:00 [medline]
PHST- 2022/05/04 00:00 [pmc-release]
AID - 10.1038/s41598-022-10065-7 [pii]
AID - 10065 [pii]
AID - 10.1038/s41598-022-10065-7 [doi]
PST - epublish
SO  - Sci Rep. 2022 May 4;12(1):7217. doi: 10.1038/s41598-022-10065-7.